Matches in SemOpenAlex for { <https://semopenalex.org/work/W2940055489> ?p ?o ?g. }
- W2940055489 endingPage "e0214580" @default.
- W2940055489 startingPage "e0214580" @default.
- W2940055489 abstract "A potentiometric whole cell biosensor based on immobilized marine bacterium, Pseudomonas carrageenovora producing κ-carrageenase and glycosulfatase enzymes for specific and direct determination of κ-carrageenan, is described. The bacterial cells were immobilized on the self-plasticized hydrogen ion (H+)-selective acrylic membrane electrode surface to form a catalytic layer. Hydrogen ionophore I was incorporated in the poly(n-butyl acrylate) [poly(nBA)] as a pH ionophore. Catalytic decomposition of κ-carrageenan by the bienzymatic cascade reaction produced neoagarobiose, an inorganic sulfate ion and a proton. The latter was detectable by H+ ion transducer for indirect potentiometric quantification of κ-carrageenan concentration. The use of a disposable screen-printed Ag/AgCl electrode (SPE) provided no cleaning requirement and enabled κ-carrageenan detection to be carried out conveniently without cross contamination in a complex food sample. The SPE-based microbial biosensor response was found to be reproducible with high reproducibility and relative standard deviation (RSD) at 2.6% (n = 3). The whole cell biosensor demonstrated a broad dynamic linear response range to κ-carrageenan from 0.2-100 ppm in 20 mM phosphate buffer saline (PBS) at pH 7.5 with a detection limit at 0.05 ppm and a Nernstian sensitivity of 58.78±0.87 mV/decade (R2 = 0.995). The biosensor showed excellent selectivity towards κ-carrageenan compared to other types of carrageenans tested e.g. ι-carrageenan and λ-carrageenan. No pretreatment to the food sample was necessary when the developed whole cell biosensor was employed for direct assay of κ-carrageenan in dairy product." @default.
- W2940055489 created "2019-04-25" @default.
- W2940055489 creator A5024011449 @default.
- W2940055489 creator A5031383187 @default.
- W2940055489 creator A5042169801 @default.
- W2940055489 creator A5062850735 @default.
- W2940055489 date "2019-04-16" @default.
- W2940055489 modified "2023-10-12" @default.
- W2940055489 title "Rapid determination of kappa-carrageenan using a biosensor from immobilized Pseudomonas carrageenovora cells" @default.
- W2940055489 cites W1429074122 @default.
- W2940055489 cites W1486985117 @default.
- W2940055489 cites W1623645098 @default.
- W2940055489 cites W1955062377 @default.
- W2940055489 cites W1967868998 @default.
- W2940055489 cites W1969191458 @default.
- W2940055489 cites W1973735531 @default.
- W2940055489 cites W1987581451 @default.
- W2940055489 cites W1990333517 @default.
- W2940055489 cites W1997803954 @default.
- W2940055489 cites W1998593017 @default.
- W2940055489 cites W2003584427 @default.
- W2940055489 cites W2006340337 @default.
- W2940055489 cites W2014725345 @default.
- W2940055489 cites W2022013585 @default.
- W2940055489 cites W2026096911 @default.
- W2940055489 cites W2028625274 @default.
- W2940055489 cites W2034292122 @default.
- W2940055489 cites W2035977114 @default.
- W2940055489 cites W2042836317 @default.
- W2940055489 cites W2049011815 @default.
- W2940055489 cites W2062298522 @default.
- W2940055489 cites W2062496252 @default.
- W2940055489 cites W2065929712 @default.
- W2940055489 cites W2069096036 @default.
- W2940055489 cites W2083514262 @default.
- W2940055489 cites W2084395854 @default.
- W2940055489 cites W2086374572 @default.
- W2940055489 cites W2088492032 @default.
- W2940055489 cites W2124226250 @default.
- W2940055489 cites W2130974392 @default.
- W2940055489 cites W2133823540 @default.
- W2940055489 cites W2137659876 @default.
- W2940055489 cites W2330386356 @default.
- W2940055489 cites W2759127374 @default.
- W2940055489 cites W298714662 @default.
- W2940055489 cites W4210986397 @default.
- W2940055489 cites W4211265116 @default.
- W2940055489 cites W4245594816 @default.
- W2940055489 cites W4255703481 @default.
- W2940055489 cites W65523500 @default.
- W2940055489 doi "https://doi.org/10.1371/journal.pone.0214580" @default.
- W2940055489 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6467376" @default.
- W2940055489 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30990847" @default.
- W2940055489 hasPublicationYear "2019" @default.
- W2940055489 type Work @default.
- W2940055489 sameAs 2940055489 @default.
- W2940055489 citedByCount "3" @default.
- W2940055489 countsByYear W29400554892020 @default.
- W2940055489 countsByYear W29400554892023 @default.
- W2940055489 crossrefType "journal-article" @default.
- W2940055489 hasAuthorship W2940055489A5024011449 @default.
- W2940055489 hasAuthorship W2940055489A5031383187 @default.
- W2940055489 hasAuthorship W2940055489A5042169801 @default.
- W2940055489 hasAuthorship W2940055489A5062850735 @default.
- W2940055489 hasBestOaLocation W29400554891 @default.
- W2940055489 hasConcept C119128265 @default.
- W2940055489 hasConcept C124614425 @default.
- W2940055489 hasConcept C13965031 @default.
- W2940055489 hasConcept C145148216 @default.
- W2940055489 hasConcept C160756335 @default.
- W2940055489 hasConcept C178790620 @default.
- W2940055489 hasConcept C185592680 @default.
- W2940055489 hasConcept C2781227287 @default.
- W2940055489 hasConcept C43617362 @default.
- W2940055489 hasConcept C55493867 @default.
- W2940055489 hasConceptScore W2940055489C119128265 @default.
- W2940055489 hasConceptScore W2940055489C124614425 @default.
- W2940055489 hasConceptScore W2940055489C13965031 @default.
- W2940055489 hasConceptScore W2940055489C145148216 @default.
- W2940055489 hasConceptScore W2940055489C160756335 @default.
- W2940055489 hasConceptScore W2940055489C178790620 @default.
- W2940055489 hasConceptScore W2940055489C185592680 @default.
- W2940055489 hasConceptScore W2940055489C2781227287 @default.
- W2940055489 hasConceptScore W2940055489C43617362 @default.
- W2940055489 hasConceptScore W2940055489C55493867 @default.
- W2940055489 hasFunder F4320322699 @default.
- W2940055489 hasIssue "4" @default.
- W2940055489 hasLocation W29400554891 @default.
- W2940055489 hasLocation W29400554892 @default.
- W2940055489 hasLocation W29400554893 @default.
- W2940055489 hasLocation W29400554894 @default.
- W2940055489 hasLocation W29400554895 @default.
- W2940055489 hasOpenAccess W2940055489 @default.
- W2940055489 hasPrimaryLocation W29400554891 @default.
- W2940055489 hasRelatedWork W1990396141 @default.
- W2940055489 hasRelatedWork W2010565079 @default.
- W2940055489 hasRelatedWork W2109764915 @default.
- W2940055489 hasRelatedWork W2163863726 @default.