Matches in SemOpenAlex for { <https://semopenalex.org/work/W2940143329> ?p ?o ?g. }
- W2940143329 endingPage "770" @default.
- W2940143329 startingPage "746" @default.
- W2940143329 abstract "Lipases are the most widely used enzymes in biocatalysis, and the most utilized method for enzyme immobilization is using hydrophobic supports at low ionic strength. This method allows the one step immobilization, purification, stabilization, and hyperactivation of lipases, and that is the main cause of their popularity. This review focuses on these lipase immobilization supports. First, the advantages of these supports for lipase immobilization will be presented and the likeliest immobilization mechanism (interfacial activation on the support surface) will be revised. Then, its main shortcoming will be discussed: enzyme desorption under certain conditions (such as high temperature, presence of cosolvents or detergent molecules). Methods to overcome this problem include physical or chemical crosslinking of the immobilized enzyme molecules or using heterofunctional supports. Thus, supports containing hydrophobic acyl chain plus epoxy, glutaraldehyde, ionic, vinylsulfone or glyoxyl groups have been designed. This prevents enzyme desorption and improved enzyme stability, but it may have some limitations, that will be discussed and some additional solutions will be proposed (e.g., chemical amination of the enzyme to have a full covalent enzyme-support reaction). These immobilized lipases may be subject to unfolding and refolding strategies to reactivate inactivated enzymes. Finally, these biocatalysts have been used in new strategies for enzyme coimmobilization, where the most stable enzyme could be reutilized after desorption of the least stable one after its inactivation." @default.
- W2940143329 created "2019-04-25" @default.
- W2940143329 creator A5022544544 @default.
- W2940143329 creator A5048099316 @default.
- W2940143329 creator A5051689348 @default.
- W2940143329 creator A5052047278 @default.
- W2940143329 creator A5059667192 @default.
- W2940143329 creator A5064041984 @default.
- W2940143329 creator A5065090209 @default.
- W2940143329 creator A5066865611 @default.
- W2940143329 date "2019-09-01" @default.
- W2940143329 modified "2023-10-18" @default.
- W2940143329 title "Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions" @default.
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