FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2940253979> ?p ?o ?g. }

• W2940253979 endingPage "e0215312" @default.
• W2940253979 startingPage "e0215312" @default.
• W2940253979 abstract "Survival rates of osteosarcoma patients could not be significantly improved by conventional chemotherapeutic treatment regimens since the introduction of high-dose chemotherapy 35 years ago. Therefore, there is a strong clinical need for new therapeutic targets and personalized treatment strategies, requiring reliable in vivo model systems for the identification and testing of potential new treatment approaches. Conventional in vivo rodent experiments face ethical issues, are time consuming and costly, being of particular relevance in orphan diseases like osteosarcoma. An attractive alternative to such animal experiments is the chicken chorioallantoic membrane (CAM) assay. The CAM is a highly vascularized, non-innervated extra-embryonic membrane that is perfectly suited for the engraftment of tumor cells. However, only few reports are available for osteosarcoma and reported data are inconsistent. Therefore, the aim of this study was the adaptation and optimization of the CAM assay for its application in osteosarcoma research. Tumor take rates and volumes of osteosarcoma that developed on the CAM were analyzed after modification of several experimental parameters, including egg windowing, CAM pretreatment, inoculation technique and many more. Eight osteosarcoma cell lines were investigated. Our optimized OS-CAM-assay was finally validated against a rat animal xenograft model. Using the cell line MNNG HOS as reference we could improve the tumor take rates from 51% to 94%, the viability of the embryos from initially 40% to >80% and achieved a threefold increase of the tumor volumes. We were able to generate solid tumors from all eight osteosarcoma cell lines used in this study and could reproduce results that were obtained using an osteosarcoma rat animal model. The CAM assay can bridge the gap between in vitro cell culture and in vivo animal experiments. As reliable in vivo model for osteosarcoma research the optimized CAM assay may speed up preclinical data collection and simplifies research on potential new agents towards personalized treatment strategies. Further, in accordance with Russell’s and Burch’s “Principles of Humane Experimental Technique” the reasonable use of this model provides a refinement by minimizing pain and suffering of animals and supports a considerable reduction and/or replacement of animal experiments." @default.
• W2940253979 created "2019-04-25" @default.
• W2940253979 creator A5004845013 @default.
• W2940253979 creator A5014049229 @default.
• W2940253979 creator A5022223987 @default.
• W2940253979 creator A5048563423 @default.
• W2940253979 creator A5067285708 @default.
• W2940253979 date "2019-04-15" @default.
• W2940253979 modified "2023-10-18" @default.
• W2940253979 title "Optimization of the chicken chorioallantoic membrane assay as reliable in vivo model for the analysis of osteosarcoma" @default.
• W2940253979 cites W145483367 @default.
• W2940253979 cites W158339772 @default.
• W2940253979 cites W1598829116 @default.
• W2940253979 cites W181426613 @default.
• W2940253979 cites W182015613 @default.
• W2940253979 cites W1971797342 @default.
• W2940253979 cites W1986690159 @default.
• W2940253979 cites W1988325237 @default.
• W2940253979 cites W2000217892 @default.
• W2940253979 cites W2006611547 @default.
• W2940253979 cites W2021849548 @default.
• W2940253979 cites W2039890442 @default.
• W2940253979 cites W2055425884 @default.
• W2940253979 cites W2057811067 @default.
• W2940253979 cites W2071389244 @default.
• W2940253979 cites W2077149593 @default.
• W2940253979 cites W2085815730 @default.
• W2940253979 cites W2093960915 @default.
• W2940253979 cites W2095599995 @default.
• W2940253979 cites W2151093049 @default.
• W2940253979 cites W2153584531 @default.
• W2940253979 cites W2311987167 @default.
• W2940253979 cites W2752949208 @default.
• W2940253979 cites W2782514648 @default.
• W2940253979 cites W335253295 @default.
• W2940253979 doi "https://doi.org/10.1371/journal.pone.0215312" @default.
• W2940253979 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6464229" @default.
• W2940253979 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30986223" @default.
• W2940253979 hasPublicationYear "2019" @default.
• W2940253979 type Work @default.
• W2940253979 sameAs 2940253979 @default.
• W2940253979 citedByCount "44" @default.
• W2940253979 countsByYear W29402539792019 @default.
• W2940253979 countsByYear W29402539792020 @default.
• W2940253979 countsByYear W29402539792021 @default.
• W2940253979 countsByYear W29402539792022 @default.
• W2940253979 countsByYear W29402539792023 @default.
• W2940253979 crossrefType "journal-article" @default.
• W2940253979 hasAuthorship W2940253979A5004845013 @default.
• W2940253979 hasAuthorship W2940253979A5014049229 @default.
• W2940253979 hasAuthorship W2940253979A5022223987 @default.
• W2940253979 hasAuthorship W2940253979A5048563423 @default.
• W2940253979 hasAuthorship W2940253979A5067285708 @default.
• W2940253979 hasBestOaLocation W29402539791 @default.
• W2940253979 hasConcept C104317684 @default.
• W2940253979 hasConcept C145103041 @default.
• W2940253979 hasConcept C150903083 @default.
• W2940253979 hasConcept C207001950 @default.
• W2940253979 hasConcept C2777760704 @default.
• W2940253979 hasConcept C2778076430 @default.
• W2940253979 hasConcept C3020616263 @default.
• W2940253979 hasConcept C502942594 @default.
• W2940253979 hasConcept C54355233 @default.
• W2940253979 hasConcept C60644358 @default.
• W2940253979 hasConcept C71924100 @default.
• W2940253979 hasConcept C81885089 @default.
• W2940253979 hasConcept C86803240 @default.
• W2940253979 hasConcept C97230052 @default.
• W2940253979 hasConceptScore W2940253979C104317684 @default.
• W2940253979 hasConceptScore W2940253979C145103041 @default.
• W2940253979 hasConceptScore W2940253979C150903083 @default.
• W2940253979 hasConceptScore W2940253979C207001950 @default.
• W2940253979 hasConceptScore W2940253979C2777760704 @default.
• W2940253979 hasConceptScore W2940253979C2778076430 @default.
• W2940253979 hasConceptScore W2940253979C3020616263 @default.
• W2940253979 hasConceptScore W2940253979C502942594 @default.
• W2940253979 hasConceptScore W2940253979C54355233 @default.
• W2940253979 hasConceptScore W2940253979C60644358 @default.
• W2940253979 hasConceptScore W2940253979C71924100 @default.
• W2940253979 hasConceptScore W2940253979C81885089 @default.
• W2940253979 hasConceptScore W2940253979C86803240 @default.
• W2940253979 hasConceptScore W2940253979C97230052 @default.
• W2940253979 hasFunder F4320320879 @default.
• W2940253979 hasFunder F4320321114 @default.
• W2940253979 hasIssue "4" @default.
• W2940253979 hasLocation W29402539791 @default.
• W2940253979 hasLocation W29402539792 @default.
• W2940253979 hasLocation W29402539793 @default.
• W2940253979 hasLocation W29402539794 @default.
• W2940253979 hasLocation W29402539795 @default.
• W2940253979 hasLocation W29402539796 @default.
• W2940253979 hasOpenAccess W2940253979 @default.
• W2940253979 hasPrimaryLocation W29402539791 @default.
• W2940253979 hasRelatedWork W1572983296 @default.
• W2940253979 hasRelatedWork W2115452170 @default.
• W2940253979 hasRelatedWork W2333578056 @default.
• W2940253979 hasRelatedWork W2370480535 @default.
• W2940253979 hasRelatedWork W2376034082 @default.

• next