FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2940308512> ?p ?o ?g. }

• W2940308512 abstract "Neurokine signaling via the release of neurally active cytokines arises from glial reactivity and is mechanistically implicated in central nervous system (CNS) pathologies such as chronic pain, trauma, neurodegenerative diseases, and complex psychiatric illnesses. Despite significant advancements in the methodologies used to conjugate, incorporate, and visualize fluorescent molecules, imaging of rare yet high potency events within the CNS is restricted by the low signal to noise ratio experienced within the CNS. The brain and spinal cord have high cellular autofluorescence, making the imaging of critical neurokine signaling and permissive transcriptional cellular events unreliable and difficult in many cases.In this manuscript, we developed a method for background-free imaging of the transcriptional events that precede neurokine signaling using targeted mRNA transcripts labeled with luminescent lanthanide chelates and imaged via time-gated microscopy. To provide examples of the usefulness this method can offer to the field, the mRNA expression of toll-like receptor 4 (TLR4) was visualized with traditional fluorescent in situ hybridization (FISH) or luminescent lanthanide chelate-based in situ hybridization (LISH) in mouse BV2 microglia or J774 macrophage phenotype cells following lipopolysaccharide stimulation. TLR4 mRNA staining using LISH- and FISH-based methods was also visualized in fixed spinal cord tissues from BALB/c mice with a chronic constriction model of neuropathic pain or a surgical sham model in order to demonstrate the application of this new methodology in CNS tissue samples.Significant increases in TLR4 mRNA expression and autofluorescence were visualized over time in mouse BV2 microglia or mouse J774 macrophage phenotype cells following lipopolysaccharide (LPS) stimulation. When imaged in a background-free environment with LISH-based detection and time-gated microscopy, increased TLR4 mRNA was observed in BV2 microglia cells 4 h following LPS stimulation, which returned to near baseline levels by 24 h. Background-free imaging of mouse spinal cord tissues with LISH-based detection and time-gated microscopy demonstrated a high degree of regional TLR4 mRNA expression in BALB/c mice with a chronic constriction model of neuropathic pain compared to the surgical sham model.Advantages offered by adopting this novel methodology for visualizing neurokine signaling with time-gated microscopy compared to traditional fluorescent microscopy are provided." @default.
• W2940308512 created "2019-04-25" @default.
• W2940308512 creator A5010886126 @default.
• W2940308512 creator A5011372652 @default.
• W2940308512 creator A5037100405 @default.
• W2940308512 creator A5037150421 @default.
• W2940308512 creator A5043680660 @default.
• W2940308512 creator A5062898531 @default.
• W2940308512 date "2019-03-21" @default.
• W2940308512 modified "2023-10-16" @default.
• W2940308512 title "Visualizing neuroinflammation with fluorescence and luminescent lanthanide-based in situ hybridization" @default.
• W2940308512 cites W1535485604 @default.
• W2940308512 cites W1588041244 @default.
• W2940308512 cites W1603094468 @default.
• W2940308512 cites W1661559393 @default.
• W2940308512 cites W1757302143 @default.
• W2940308512 cites W1960895270 @default.
• W2940308512 cites W1963711291 @default.
• W2940308512 cites W1978059875 @default.
• W2940308512 cites W1981194677 @default.
• W2940308512 cites W1997660163 @default.
• W2940308512 cites W1999701956 @default.
• W2940308512 cites W2034088074 @default.
• W2940308512 cites W2035605437 @default.
• W2940308512 cites W2038300605 @default.
• W2940308512 cites W2042183116 @default.
• W2940308512 cites W2051093466 @default.
• W2940308512 cites W2051314297 @default.
• W2940308512 cites W2052317102 @default.
• W2940308512 cites W2058020736 @default.
• W2940308512 cites W2062238523 @default.
• W2940308512 cites W2062996871 @default.
• W2940308512 cites W2073427034 @default.
• W2940308512 cites W2078873019 @default.
• W2940308512 cites W2086186229 @default.
• W2940308512 cites W2087243986 @default.
• W2940308512 cites W2088341766 @default.
• W2940308512 cites W2097086441 @default.
• W2940308512 cites W2108729511 @default.
• W2940308512 cites W2114395128 @default.
• W2940308512 cites W2123355863 @default.
• W2940308512 cites W2123577868 @default.
• W2940308512 cites W2125333702 @default.
• W2940308512 cites W2126575561 @default.
• W2940308512 cites W2134557769 @default.
• W2940308512 cites W2135235221 @default.
• W2940308512 cites W2140441724 @default.
• W2940308512 cites W2162872622 @default.
• W2940308512 cites W2166475990 @default.
• W2940308512 cites W2167011761 @default.
• W2940308512 cites W2167742884 @default.
• W2940308512 cites W2179059031 @default.
• W2940308512 cites W2280077905 @default.
• W2940308512 cites W2325078067 @default.
• W2940308512 cites W2327941755 @default.
• W2940308512 cites W2331659843 @default.
• W2940308512 cites W2342085519 @default.
• W2940308512 cites W2343588953 @default.
• W2940308512 cites W2419095419 @default.
• W2940308512 cites W2513707141 @default.
• W2940308512 cites W2520677640 @default.
• W2940308512 cites W2550287592 @default.
• W2940308512 cites W2622909440 @default.
• W2940308512 cites W2793231345 @default.
• W2940308512 doi "https://doi.org/10.1186/s12974-019-1451-2" @default.
• W2940308512 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6427895" @default.
• W2940308512 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30898121" @default.
• W2940308512 hasPublicationYear "2019" @default.
• W2940308512 type Work @default.
• W2940308512 sameAs 2940308512 @default.
• W2940308512 citedByCount "6" @default.
• W2940308512 countsByYear W29403085122020 @default.
• W2940308512 countsByYear W29403085122021 @default.
• W2940308512 countsByYear W29403085122023 @default.
• W2940308512 crossrefType "journal-article" @default.
• W2940308512 hasAuthorship W2940308512A5010886126 @default.
• W2940308512 hasAuthorship W2940308512A5011372652 @default.
• W2940308512 hasAuthorship W2940308512A5037100405 @default.
• W2940308512 hasAuthorship W2940308512A5037150421 @default.
• W2940308512 hasAuthorship W2940308512A5043680660 @default.
• W2940308512 hasAuthorship W2940308512A5062898531 @default.
• W2940308512 hasBestOaLocation W29403085121 @default.
• W2940308512 hasConcept C104317684 @default.
• W2940308512 hasConcept C105580179 @default.
• W2940308512 hasConcept C142724271 @default.
• W2940308512 hasConcept C153911025 @default.
• W2940308512 hasConcept C169760540 @default.
• W2940308512 hasConcept C203014093 @default.
• W2940308512 hasConcept C2776070231 @default.
• W2940308512 hasConcept C2776914184 @default.
• W2940308512 hasConcept C2777107010 @default.
• W2940308512 hasConcept C2779830541 @default.
• W2940308512 hasConcept C529278444 @default.
• W2940308512 hasConcept C55493867 @default.
• W2940308512 hasConcept C62478195 @default.
• W2940308512 hasConcept C71924100 @default.
• W2940308512 hasConcept C81439078 @default.
• W2940308512 hasConcept C86803240 @default.
• W2940308512 hasConcept C87753298 @default.
• W2940308512 hasConcept C95444343 @default.

• next