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• W2940807977 abstract "PDF HTML阅读 XML下载 导出引用 引用提醒 添加嗜酸乳杆菌对凡纳滨对虾生长、酶活性及相关酶mRNA表达的影响 DOI: 作者: 作者单位: 1. 广东海洋大学 水产动物营养与饲料实验室, 广东 湛江 524088;2. 农业农村部南方水产与畜禽饲料重点实验室, 广东 湛江 524074 作者简介: 李军亮(1991-),男,硕士研究生,研究方向为水产动物营养与饲料.E-mail:junliangli132@163.com 通讯作者: 中图分类号: S968 基金项目: 广东省科技厅社会发展领域科技计划项目（2013B021100017）；农业部南方水产与畜禽饲料重点实验室项目. Effects of Lactobacillus acidophilus supplementation on the growth, enzyme activities, and mRNA expression of disease-resistance related enzymes of juvenile Litopenaeus vannamei Author: Affiliation: 1. Laboratory of Aquatic Animal Nutrition and Feed, Guangdong Ocean University, Zhanjiang 524088, China;2. Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agri-culture and Rural Affairs, Zhanjiang 524074, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:本实验旨在研究饲料中添加嗜酸乳杆菌（）幼虾生长、非特异性免疫酶活性、抗病力和相关酶mRNA表达的影响。选取840尾初始均重为（0.58±0.01）g的凡纳滨对虾幼虾为研究对象，分为7个处理，每个处理3个重复。投喂添加不同比例（0%，0.1%，0.2%，0.4%，0.6%，0.8%，1.0%）嗜酸乳杆菌的饲料，养殖期8周。结果显示：添加不同比例嗜酸乳杆菌对凡纳滨对虾的成活率无显著影响（<0.05），在0.4%组达到最大值；当添加0.2%时，饲料系数最低，其他各组显著低于对照组（<0.05），而蛋白质效率的变化规律则与饲料系数相反。血清酚氧化物酶（phenoloxidase，PO）、碱性磷酸酶（alkaline phosphatase，AKP）和酸性磷酸酶（acid phosphatase，ACP）活性随添加量的增加先升高后降低，分别为0.4%、0.1%和0.2%时，达到最大值。过氧化氢酶（catalase，CAT）、溶菌酶（lysozyme，LZM）和超氧化物歧化酶（superoxide dismutase，SOD）活性，实验组均显著高于对照组（<0.05）。哈维弧菌（<0.05）。以增重率（weight gain rate，WGR）为判断依据，根据折线模型得出，饲料中添加0.23%嗜酸乳杆菌可显著促进凡纳滨对虾生长，并提高非特异性免疫酶活性。 Abstract:An 8-week feeding trial was carried out to investigate the effects of supplementation on the growth performance, nonspecific immunity-related enzyme activities, and disease resistance-related enzyme mRNA expression in juvenile , with initial average weight of 0.58±0.01 g, was randomly assigned to 7 groups in triplicates, with 40 individuals per replicate. Seven isonitrogenous and isolipid diets were supplemented with 0%, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, and 1.0% in a basal diet, respectively. Following results were observed:nonsignificant difference was found in survival among all the groups (>0.05). Weight gain rate (WGR) and specific growth rate (SGR) increased gradually as dietary supplementation increased up to 0.4%, and then showed decline (<0.05). The minimum feed conversion rate (FCR) was observed on feed supplementation with 0.2% , which was significantly lower than that in the other groups (<0.05), while protein efficiency rate (PER) showed the opposite trend. Serum phenoloxidase (PO), alkaline phosphatase (AKP), and acid phosphatase (ACP) activities increased gradually with increasing dietary levels, and then gradually declined. The maximum values of PO, AKP, and ACP were found in the 0.4%, 0.1%, and 0.2% supplementation groups, respectively (<0.05). Activities of serum catalase (CAT), lysozyme (LSZ), and superoxide dismutase (SOD) of all treatment groups were significantly higher than that of the control group (0%) (<0.05). Gene expression levels of CAT and LZM in all treatment groups were significantly up-regulated when compared with the control group (<0.05). At the end of the feeding trial, the shrimps were challenged by for 96 h. Post-challenge survival rate increased with increasing dietary <0.05). Based on the broken-line regression analysis of WGR, when feed was supplemented with 0.23% of Litopenaeus vannamei were found to significantly improve. 参考文献 相似文献 引证文献" @default.
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• W2940807977 title "Effects of Lactobacillus acidophilus supplementation on the growth, enzyme activities, and mRNA expression of disease-resistance related enzymes of juvenile Litopenaeus vannamei" @default.
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