Matches in SemOpenAlex for { <https://semopenalex.org/work/W2940968906> ?p ?o ?g. }
- W2940968906 abstract "Abstract Ribosomal protein (RP) genes locate near the replication origin ( oriC ) in fast-growing bacteria, which is thought to have been selected as a translation optimization strategy. Relocation of S10-spc-α locus (S10), which codes for most of the RP, to ectopic genomic positions shows that its relative distance to the oriC correlates to a reduction on its dosage, its expression, and bacterial growth rate. Deep-sequencing revealed that S10 relocation altered chromosomal replication dynamics and genome-wide transcription. Such changes increased as a function of oriC -S10 distance. Strikingly, in this work we observed that protein production capacity was independent of S10 position. Since RP constitute a large proportion of cell mass, lower S10 dosage could lead to changes in macromolecular crowding, impacting cell physiology. Accordingly, cytoplasm fluidity was higher in mutants where S10 is most distant from oriC . In hyperosmotic conditions, when crowding differences are minimized, the growth rate and replication dynamics were highly alleviated in these strains. Therefore, on top of its essential function in translation, RP genomic location contributes to sustain optimal macromolecular crowding. This is a novel mechanism coordinating DNA replication with bacterial growth." @default.
- W2940968906 created "2019-05-03" @default.
- W2940968906 creator A5006612133 @default.
- W2940968906 creator A5008940362 @default.
- W2940968906 creator A5016816655 @default.
- W2940968906 creator A5020802740 @default.
- W2940968906 creator A5026674371 @default.
- W2940968906 creator A5028718269 @default.
- W2940968906 creator A5033278912 @default.
- W2940968906 creator A5039866388 @default.
- W2940968906 creator A5043476240 @default.
- W2940968906 creator A5050146755 @default.
- W2940968906 creator A5072020647 @default.
- W2940968906 creator A5089191821 @default.
- W2940968906 date "2019-04-25" @default.
- W2940968906 modified "2023-10-17" @default.
- W2940968906 title "Macromolecular crowding links ribosomal protein gene dosage to growth rate inVibrio cholerae" @default.
- W2940968906 cites W1489859347 @default.
- W2940968906 cites W1501921723 @default.
- W2940968906 cites W1540505601 @default.
- W2940968906 cites W1888354418 @default.
- W2940968906 cites W1899251850 @default.
- W2940968906 cites W1924219513 @default.
- W2940968906 cites W1925362388 @default.
- W2940968906 cites W1966124059 @default.
- W2940968906 cites W1967531530 @default.
- W2940968906 cites W1969685924 @default.
- W2940968906 cites W1978110792 @default.
- W2940968906 cites W1978468198 @default.
- W2940968906 cites W1985161570 @default.
- W2940968906 cites W1987073458 @default.
- W2940968906 cites W1990507322 @default.
- W2940968906 cites W1992033675 @default.
- W2940968906 cites W1997272729 @default.
- W2940968906 cites W2007609997 @default.
- W2940968906 cites W200778161 @default.
- W2940968906 cites W2013041427 @default.
- W2940968906 cites W2016263615 @default.
- W2940968906 cites W2020426274 @default.
- W2940968906 cites W2032276769 @default.
- W2940968906 cites W2035327900 @default.
- W2940968906 cites W2052195371 @default.
- W2940968906 cites W2058729834 @default.
- W2940968906 cites W2060598691 @default.
- W2940968906 cites W2061228928 @default.
- W2940968906 cites W2063480230 @default.
- W2940968906 cites W2068175266 @default.
- W2940968906 cites W2080938078 @default.
- W2940968906 cites W2090419763 @default.
- W2940968906 cites W2090433162 @default.
- W2940968906 cites W2099519840 @default.
- W2940968906 cites W2100638358 @default.
- W2940968906 cites W2107996465 @default.
- W2940968906 cites W2111354165 @default.
- W2940968906 cites W2119604950 @default.
- W2940968906 cites W2121980126 @default.
- W2940968906 cites W2124985265 @default.
- W2940968906 cites W2134526812 @default.
- W2940968906 cites W2137119243 @default.
- W2940968906 cites W2140197221 @default.
- W2940968906 cites W2143420371 @default.
- W2940968906 cites W2144657744 @default.
- W2940968906 cites W2149633455 @default.
- W2940968906 cites W2157248714 @default.
- W2940968906 cites W2158804744 @default.
- W2940968906 cites W2160594875 @default.
- W2940968906 cites W2161435888 @default.
- W2940968906 cites W2163029786 @default.
- W2940968906 cites W2171451207 @default.
- W2940968906 cites W2179438025 @default.
- W2940968906 cites W2231401550 @default.
- W2940968906 cites W2327011266 @default.
- W2940968906 cites W2338496560 @default.
- W2940968906 cites W2409577334 @default.
- W2940968906 cites W2468464864 @default.
- W2940968906 cites W2587727933 @default.
- W2940968906 cites W2593464858 @default.
- W2940968906 cites W2604014518 @default.
- W2940968906 cites W2745153408 @default.
- W2940968906 cites W2769190237 @default.
- W2940968906 cites W2790103938 @default.
- W2940968906 cites W2790928182 @default.
- W2940968906 cites W2792611945 @default.
- W2940968906 cites W2802362769 @default.
- W2940968906 cites W2806684614 @default.
- W2940968906 cites W2809092342 @default.
- W2940968906 cites W2884295707 @default.
- W2940968906 cites W2950985970 @default.
- W2940968906 cites W2951087054 @default.
- W2940968906 cites W3100301220 @default.
- W2940968906 doi "https://doi.org/10.1101/619304" @default.
- W2940968906 hasPublicationYear "2019" @default.
- W2940968906 type Work @default.
- W2940968906 sameAs 2940968906 @default.
- W2940968906 citedByCount "0" @default.
- W2940968906 crossrefType "posted-content" @default.
- W2940968906 hasAuthorship W2940968906A5006612133 @default.
- W2940968906 hasAuthorship W2940968906A5008940362 @default.
- W2940968906 hasAuthorship W2940968906A5016816655 @default.
- W2940968906 hasAuthorship W2940968906A5020802740 @default.