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• W2941431105 abstract "You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder I (MP71)1 Apr 2019MP71-02 HYPERMOTILITY OF ESCHERICHIA COLI ISOLATED FROM THE UROTHELIUM OF POSTMENOPAUSAL WOMEN WITH RECURRENT URINARY TRACT INFECTION. Cristian Trejo*, Vivian Nguyen, Nicole J. DeNisco, Kelli L. Palmer, and Philippe Zimmern Cristian Trejo*Cristian Trejo* More articles by this author , Vivian NguyenVivian Nguyen More articles by this author , Nicole J. DeNiscoNicole J. DeNisco More articles by this author , Kelli L. PalmerKelli L. Palmer More articles by this author , and Philippe ZimmernPhilippe Zimmern More articles by this author View All Author Informationhttps://doi.org/10.1097/01.JU.0000557114.92349.3eAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVES: Community-acquired urinary tract infections (UTIs) are the most commonly reported infection in adults and have a high rate of recurrence in postmenopausal women (1). In murine models, uropathogenic Escherichia coli (UPEC) invades the bladder urothelium and forms intracellular bacterial reservoirs (IBCs) as a key part of the infection cycle. However, UPEC strains had not been previously isolated from the bladder wall of human RUTI patients (2). The goal of this study was to isolate UPEC strains from bladder wall biopsies of postmenopausal RUTI patients ungergoing cystoscopy with fulguration of trigonitis (CFT) in order to study the virulence factors and adaptations important for effective colonization of the human bladder METHODS: Following IRB approval, bladder biopsies were obtained from consenting women meeting study criteria for antibiotic-refractory RUTIs who had elected CFT. Biopsies were obtained in the operating room under anesthesia, placed directly in a solution of 100 ug/mL penicillin and gentamicin, and incubated for >2 hours at room temperature to eliminate extracellular bacteria. Tissue was then washed with sterile 1X PBS three times, homogenized, and plated on Chromagar to culture bacteria. UPEC tissue isolates were obtained from two patients. Swim assays in 3% LB agar were performed on two tissue isolates from each patient as well as the lab E. coli strain DH5α as a control. Motility was recorded 24 and 48 hours post-inoculation. RESULTS: The figure depicts representative swim assay results at 24 and 48 hours post-inoculation for isolates from patients PNK004 (A) and PNK006 (B). All UPEC tissue isolates demonstrated enhanced swimming motility at both 24 and 48 hours post-inoculation compared to the control DH5α E. coli strain. CONCLUSIONS: Previously, flagella-mediated swimming motility was found to be important for competitive colonization of the murine urinary tract by sepsis isolate CFT073 (3). In this preliminary study, the UPEC tissue isolates from RUTI patients demonstrated enhanced swimming motility compared to DH5α. These findings suggest that flagella-mediated swimming motility may be an important virulence factor for UPEC during human RUTI. Further work is needed to understand the molecular basis of hypermotility in these isolates. REFERENCES 1. J Infect Dis. 2001 Mar 1; Suppl 1, S1-4 2. Proc Natl Acad Sci U S A. 2004 Feb 3; 101:1333-1338 3. Infection and Immunity 2005 Nov; 73(11): 7644-7656 Source of Funding: None Bryan, TX; Richardson, TX; Dallas, TX© 2019 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e1046-e1046 Advertisement Copyright & Permissions© 2019 by American Urological Association Education and Research, Inc.MetricsAuthor Information Cristian Trejo* More articles by this author Vivian Nguyen More articles by this author Nicole J. DeNisco More articles by this author Kelli L. Palmer More articles by this author Philippe Zimmern More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
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• W2941431105 title "MP71-02 HYPERMOTILITY OF ESCHERICHIA COLI ISOLATED FROM THE UROTHELIUM OF POSTMENOPAUSAL WOMEN WITH RECURRENT URINARY TRACT INFECTION." @default.
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