FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2941881917> ?p ?o ?g. }

• W2941881917 abstract "Isatis indigotica, a traditional Chinese medicine, produces a variety of active ingredients. However, little is known about the key genes and corresponding expression profiling involved in the biosynthesis pathways of these ingredients. Quantitative real-time polymerase chain reaction (qRT-PCR) is a powerful, commonly-used method for gene expression analysis, but the accuracy of the quantitative data produced depends on the appropriate selection of reference genes.In this study, the systematic analysis of the reference genes was performed for quantitative real-Time PCR normalization in I. indigotica. We selected nine candidate reference genes, including six traditional housekeeping genes (ACT, α-TUB, β-TUB, UBC, CYP, and EF1-α), and three newly stable internal control genes (MUB, TIP41, and RPL) from a transcriptome dataset of I. indigotica, and evaluated their expression stabilities in different tissues (root, stem, leaf, and petiole) and leaves exposed to three abiotic treatments (low-nitrogen, ABA, and MeJA) using geNorm, NormFinder, BestKeeper, and comprehensive RefFind algorithms. The results demonstrated that MUB and EF1-α were the two most stable reference genes for all samples. TIP41 as the optimal reference gene for low-nitrogen stress and MeJA treatment, while ACT had the highest ranking for ABA treatment and CYP was the most suitable for different tissues.The results revealed that the selection and validation of appropriate reference genes for normalizing data is mandatory to acquire accurate quantification results. The necessity of specific internal control for specific conditions was also emphasized. Furthermore, this work will provide valuable information to enhance further research in gene function and molecular biology on I. indigotica and other related species." @default.
• W2941881917 created "2019-05-03" @default.
• W2941881917 creator A5002782106 @default.
• W2941881917 creator A5008390450 @default.
• W2941881917 creator A5024068533 @default.
• W2941881917 creator A5039311362 @default.
• W2941881917 creator A5049870701 @default.
• W2941881917 creator A5063377124 @default.
• W2941881917 creator A5074483335 @default.
• W2941881917 creator A5088695928 @default.
• W2941881917 date "2019-03-25" @default.
• W2941881917 modified "2023-10-16" @default.
• W2941881917 title "Selection of reference genes for the quantitative real-time PCR normalization of gene expression in Isatis indigotica fortune" @default.
• W2941881917 cites W113522983 @default.
• W2941881917 cites W1545201063 @default.
• W2941881917 cites W1577577364 @default.
• W2941881917 cites W1577822803 @default.
• W2941881917 cites W1899703492 @default.
• W2941881917 cites W1969831304 @default.
• W2941881917 cites W1973632603 @default.
• W2941881917 cites W1973649249 @default.
• W2941881917 cites W1978897742 @default.
• W2941881917 cites W1979138510 @default.
• W2941881917 cites W1981509058 @default.
• W2941881917 cites W1984861884 @default.
• W2941881917 cites W1986007439 @default.
• W2941881917 cites W1995931510 @default.
• W2941881917 cites W2008183439 @default.
• W2941881917 cites W2010146791 @default.
• W2941881917 cites W2024166332 @default.
• W2941881917 cites W2029632139 @default.
• W2941881917 cites W2038338661 @default.
• W2941881917 cites W2040685881 @default.
• W2941881917 cites W2042119125 @default.
• W2941881917 cites W2048865019 @default.
• W2941881917 cites W2051775778 @default.
• W2941881917 cites W2056389516 @default.
• W2941881917 cites W2057464020 @default.
• W2941881917 cites W2064300963 @default.
• W2941881917 cites W2075033706 @default.
• W2941881917 cites W2084616269 @default.
• W2941881917 cites W2085587146 @default.
• W2941881917 cites W2085700736 @default.
• W2941881917 cites W2086491289 @default.
• W2941881917 cites W2089269320 @default.
• W2941881917 cites W2099824652 @default.
• W2941881917 cites W2100305481 @default.
• W2941881917 cites W2105423509 @default.
• W2941881917 cites W2107277218 @default.
• W2941881917 cites W2111427334 @default.
• W2941881917 cites W2112982595 @default.
• W2941881917 cites W2117128136 @default.
• W2941881917 cites W2117468560 @default.
• W2941881917 cites W2119284644 @default.
• W2941881917 cites W2127577698 @default.
• W2941881917 cites W2135598172 @default.
• W2941881917 cites W2150534844 @default.
• W2941881917 cites W2163254642 @default.
• W2941881917 cites W2168745370 @default.
• W2941881917 cites W2255872256 @default.
• W2941881917 cites W2262335420 @default.
• W2941881917 cites W2263435702 @default.
• W2941881917 cites W2333461586 @default.
• W2941881917 cites W2342387702 @default.
• W2941881917 cites W2345903473 @default.
• W2941881917 cites W2464216695 @default.
• W2941881917 cites W2579941947 @default.
• W2941881917 cites W2612724553 @default.
• W2941881917 cites W2616334557 @default.
• W2941881917 cites W2751165022 @default.
• W2941881917 cites W2762312627 @default.
• W2941881917 cites W2808910079 @default.
• W2941881917 cites W2885116343 @default.
• W2941881917 cites W2949702575 @default.
• W2941881917 cites W1964184380 @default.
• W2941881917 cites W2558760339 @default.
• W2941881917 doi "https://doi.org/10.1186/s12867-019-0126-y" @default.
• W2941881917 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6434783" @default.
• W2941881917 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30909859" @default.
• W2941881917 hasPublicationYear "2019" @default.
• W2941881917 type Work @default.
• W2941881917 sameAs 2941881917 @default.
• W2941881917 citedByCount "25" @default.
• W2941881917 countsByYear W29418819172020 @default.
• W2941881917 countsByYear W29418819172021 @default.
• W2941881917 countsByYear W29418819172022 @default.
• W2941881917 countsByYear W29418819172023 @default.
• W2941881917 crossrefType "journal-article" @default.
• W2941881917 hasAuthorship W2941881917A5002782106 @default.
• W2941881917 hasAuthorship W2941881917A5008390450 @default.
• W2941881917 hasAuthorship W2941881917A5024068533 @default.
• W2941881917 hasAuthorship W2941881917A5039311362 @default.
• W2941881917 hasAuthorship W2941881917A5049870701 @default.
• W2941881917 hasAuthorship W2941881917A5063377124 @default.
• W2941881917 hasAuthorship W2941881917A5074483335 @default.
• W2941881917 hasAuthorship W2941881917A5088695928 @default.
• W2941881917 hasBestOaLocation W29418819172 @default.
• W2941881917 hasConcept C104317684 @default.
• W2941881917 hasConcept C136886441 @default.
• W2941881917 hasConcept C144024400 @default.

• next