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- W2942019417 abstract "In this work, we found that the Lrp/AsnC family protein SACE_5717 negatively regulated erythromycin biosynthesis in S. erythraea. Disruption of SACE_5717 led to a 27% improvement in the yield of erythromycin in S. erythraea A226. SACE_5717 directly repressed its own gene expression, as well as that of the adjacent gene SACE_5716 by binding to the target sequence 5'-GAACGTTCGCCGTCACGCC-3'. The predicted LysE superfamily protein SACE_5716 directly influenced the export of lysine, histidine, threonine and glycine in S. erythraea. Arginine, tyrosine and tryptophan were characterized as the effectors of SACE_5717 by weakening the binding affinity of SACE_5717. In the industrial S. erythraea WB strain, deletion of SACE_5717 (WBΔSACE_5717) increased erythromycin yield by 20%, and by 36% when SACE_5716 was overexpressed in WBΔSACE_5717 (WBΔSACE_5717/5716). In large-scale 5-L fermentation experiment, erythromycin yield in the engineered strain WBΔSACE_5717/5716 reached 4686 mg/L, a 41% enhancement over 3323 mg/L of the parent WB strain." @default.
- W2942019417 created "2019-05-03" @default.
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- W2942019417 date "2019-07-01" @default.
- W2942019417 modified "2023-10-16" @default.
- W2942019417 title "Characterization and engineering of the Lrp/AsnC family regulator SACE_5717 for erythromycin overproduction in <i>Saccharopolyspora erythraea</i>" @default.
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- W2942019417 doi "https://doi.org/10.1007/s10295-019-02178-2" @default.
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