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• W2942163931 abstract "Solar-induced chlorophyll a Fluorescence (SIF), which is distributed over a relatively broad (~200 nm) spectral range, is a signal intricately connected to the efficiency of photosynthesis and is now observable from space. Variants of the Fraunhofer Line Depth/Discriminator (FLD) method are used as the basis of retrieval algorithms for estimating SIF from space. Although typically unobserved directly, recent advances in FLD-based algorithms now facilitate the prediction (by model inversion) of the canopy emitted fluorescence spectrum from the discrete-feature FLD retrievals. Here we present first canopy scale measurements of chlorophyll a fluorescence spectra emitted from Scots pine at two times of year, and also from a lingonberry dominated understory. We used a high power multispectral Light Emitting Diode (LED) array to illuminate the respective canopies at night and measured under standardised conditions using a field spectrometer mounted in the nadir position above the canopy. We refer to the technique, which facilitates the in situ upscaling of a commonly measured leaf scale quantity to the canopy, as nocturnal LED-Induced chlorophyll a Fluorescence (LEDIF). The shape of the LEDIF spectra was dependant on the colour of the excitation light and also on the dominant species. Because we measured pine at two different times of year we were also able to show an increase in the canopy scale apparent quantum yield of fluorescence which was consistent with leaf-level increase in fluorescence yield recorded with a monitoring PAM fluorometer. The automation of the LEDIF technique could be used to estimate seasonal changes in canopy fluorescence spectra and yield from fixed or mobile platforms and provide a window into functional traits across species and architectures. LEDIF could also be used to evaluate FLD and inversion-based retrievals of canopy spectra, as well as different irradiance normalisation schemes typically applied to SIF data to account for the dependence of SIF on ambient light conditions." @default.
• W2942163931 created "2019-05-03" @default.
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• W2942163931 date "2019-09-01" @default.
• W2942163931 modified "2023-10-14" @default.
• W2942163931 title "Nocturnal Light Emitting Diode Induced Fluorescence (LEDIF): A new technique to measure the chlorophyll a fluorescence emission spectral distribution of plant canopies in situ" @default.
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• W2942163931 doi "https://doi.org/10.1016/j.rse.2019.03.030" @default.
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