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- W2942301938 abstract "The polymorphism in leptin (LEP 2548A) seems to influence obesity among others genes. The aim of this study is to investigate the effect of the G2548A polymorphism on body mass index. We included 79 people from Slovakia with some genetic relatedness and used barrels kit to isolate the genomic DNA from an adenoblast swab- from the salivary. PCR products were amplified by pursued polymorphisms and G2548A, we restriction-analyzed them and then we identified the specific fragments describing the presence of chosen SNP polymorphism by the agarose electrophoresis, to analyze SNP polymorphism by PCR-RFLP method.
 The LEP gene had increased frequency of G allele (0.5506). The most common genotype occurring in the gene LEP was heterozygous genotype (AG) and the least frequent genotype in LEP was AA (0.1899). Taking the age into account the BMI is higher if the G allele occurs in the LEP gene. Moreover, if the G allele genotype was situated in dominant form, then the highest average BMI was present.
 According to the results we can assume that the AA genotype (LEP) has a protective effect on the prevalence of obesity compared to the other genotypes." @default.
- W2942301938 created "2019-05-03" @default.
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- W2942301938 date "2016-02-18" @default.
- W2942301938 modified "2023-09-26" @default.
- W2942301938 title "Effect of G2548A polymorphism in the leptin gene on the BMI level in human population" @default.
- W2942301938 doi "https://doi.org/10.34101/actaagrar/68/1762" @default.
- W2942301938 hasPublicationYear "2016" @default.
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