Matches in SemOpenAlex for { <https://semopenalex.org/work/W2942321874> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2942321874 abstract "Potato leafroll luteovirus is an aphid-transmissible virus which has isometric particles and is confined to the phloem tissue of infected plants. Its multiplication was investigated by using plant protoplasts as a model system. In protoplasts, net accumulation of PLRV ceased at approximately 48 his post-inoculation. Virus-specific products were detectable 15 hrs or more post-inoculation and remained detectable at approximately 100 hrs postinoculation. The amount of PLRV accumulated depended on the conditions in which protoplasts were incubated. Incubation at 25°C rather than 20°C and incubation in the dark for a period rather than continuous light resulted in more PLRV accumulation. RNA extracted from PLRV-infected protoplasts was identical on northern blots to that extracted from leaf tissue of PLRV-infected Maris Piper potato plants. Northern blots of RNA from other plants, some resistant, some susceptible to PLRV multiplication, were very similar. Resistant plants appeared to contain smaller quantities of subgenomic RNA. The genes at the 3'-end of the genome are expressed by translation of a subgenomic RNA. This was mapped to position 3376 on the PLRV genome and is therefore 2505 nucleotides long. The untranslated leader sequence of 212 nucleotides contains some putative promoter sequences although not in the same order as described for other viruses. A sequence of 8 nucleotides at the 5'-end of the genomic RNA was found to be repeated at the 5'-end of subgenomic RNA. The complement of this sequence may form part of an internal initiation site for the viral replicase complex in the minus strand RNA. The possibility of the untranslated leader sequence containing several promoters for both subgenomic RNA synthesis and ORF expression is discussed. Protoplast lysates contained a component that sedimented nearer the top of a sucrose gradient than virus particles. This contained subgenomic RNA and was detectable by ELISA but not by electron microscopy. It was not present in extracts of PLRV-infected plant tissue or in preparations of purified virus particles and may therefore be an unstable structure possibly - playing a role in particle assembly." @default.
- W2942321874 created "2019-05-03" @default.
- W2942321874 creator A5075327410 @default.
- W2942321874 date "1993-01-01" @default.
- W2942321874 modified "2023-09-23" @default.
- W2942321874 title "Molecular studies of potato leafroll luteovirus multiplication" @default.
- W2942321874 hasPublicationYear "1993" @default.
- W2942321874 type Work @default.
- W2942321874 sameAs 2942321874 @default.
- W2942321874 citedByCount "0" @default.
- W2942321874 crossrefType "dissertation" @default.
- W2942321874 hasAuthorship W2942321874A5075327410 @default.
- W2942321874 hasConcept C104317684 @default.
- W2942321874 hasConcept C109110057 @default.
- W2942321874 hasConcept C153911025 @default.
- W2942321874 hasConcept C159047783 @default.
- W2942321874 hasConcept C189819185 @default.
- W2942321874 hasConcept C2522874641 @default.
- W2942321874 hasConcept C2775976403 @default.
- W2942321874 hasConcept C2777066214 @default.
- W2942321874 hasConcept C2778580959 @default.
- W2942321874 hasConcept C2781191690 @default.
- W2942321874 hasConcept C54355233 @default.
- W2942321874 hasConcept C59822182 @default.
- W2942321874 hasConcept C67705224 @default.
- W2942321874 hasConcept C84148353 @default.
- W2942321874 hasConcept C86803240 @default.
- W2942321874 hasConceptScore W2942321874C104317684 @default.
- W2942321874 hasConceptScore W2942321874C109110057 @default.
- W2942321874 hasConceptScore W2942321874C153911025 @default.
- W2942321874 hasConceptScore W2942321874C159047783 @default.
- W2942321874 hasConceptScore W2942321874C189819185 @default.
- W2942321874 hasConceptScore W2942321874C2522874641 @default.
- W2942321874 hasConceptScore W2942321874C2775976403 @default.
- W2942321874 hasConceptScore W2942321874C2777066214 @default.
- W2942321874 hasConceptScore W2942321874C2778580959 @default.
- W2942321874 hasConceptScore W2942321874C2781191690 @default.
- W2942321874 hasConceptScore W2942321874C54355233 @default.
- W2942321874 hasConceptScore W2942321874C59822182 @default.
- W2942321874 hasConceptScore W2942321874C67705224 @default.
- W2942321874 hasConceptScore W2942321874C84148353 @default.
- W2942321874 hasConceptScore W2942321874C86803240 @default.
- W2942321874 hasLocation W29423218741 @default.
- W2942321874 hasOpenAccess W2942321874 @default.
- W2942321874 hasPrimaryLocation W29423218741 @default.
- W2942321874 hasRelatedWork W1545851729 @default.
- W2942321874 hasRelatedWork W1924104928 @default.
- W2942321874 hasRelatedWork W1986286046 @default.
- W2942321874 hasRelatedWork W2007067261 @default.
- W2942321874 hasRelatedWork W2020267568 @default.
- W2942321874 hasRelatedWork W2045771489 @default.
- W2942321874 hasRelatedWork W2047165161 @default.
- W2942321874 hasRelatedWork W2056412935 @default.
- W2942321874 hasRelatedWork W2065892420 @default.
- W2942321874 hasRelatedWork W2067995209 @default.
- W2942321874 hasRelatedWork W2083020885 @default.
- W2942321874 hasRelatedWork W2106687784 @default.
- W2942321874 hasRelatedWork W2127966531 @default.
- W2942321874 hasRelatedWork W2129592399 @default.
- W2942321874 hasRelatedWork W2132554700 @default.
- W2942321874 hasRelatedWork W2151237892 @default.
- W2942321874 hasRelatedWork W2152188010 @default.
- W2942321874 hasRelatedWork W2163189645 @default.
- W2942321874 hasRelatedWork W2267940908 @default.
- W2942321874 hasRelatedWork W2327607690 @default.
- W2942321874 isParatext "false" @default.
- W2942321874 isRetracted "false" @default.
- W2942321874 magId "2942321874" @default.
- W2942321874 workType "dissertation" @default.