FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2942341256> ?p ?o ?g. }

• W2942341256 abstract "You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology III (MP63)1 Apr 2019MP63-06 COMPARATIVE DETERMINATION OF TERT MUTATIONS IN URINARY SEDIMENT DNA BY NGS AND DDPCR FOR BLADDER CANCER DETECTION Friedrich Stoeckel, Karsten Salomo, Stasik Sebastian, Christian Thiede, Mario Menschikowski, Ulrike Heberling, Manfred P. Wirth, and Susanne Fuessel* Friedrich StoeckelFriedrich Stoeckel More articles by this author , Karsten SalomoKarsten Salomo More articles by this author , Stasik SebastianStasik Sebastian More articles by this author , Christian ThiedeChristian Thiede More articles by this author , Mario MenschikowskiMario Menschikowski More articles by this author , Ulrike HeberlingUlrike Heberling More articles by this author , Manfred P. WirthManfred P. Wirth More articles by this author , and Susanne Fuessel*Susanne Fuessel* More articles by this author View All Author Informationhttps://doi.org/10.1097/01.JU.0000556849.64618.1dAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVES: The determination of the two abundant point-mutations C228T and C250T in the TERT promoter in urine samples could potentially enhance the non-invasive detection of bladder cancer (BCa). Therefore, the applicability of two molecular biological detection methods - next-generation sequencing (NGS) and digital droplet PCR (ddPCR) - was compared with the voided urine cytology (VUC). METHODS: Genomic DNA was isolated from urinary sediments from 49 histologically positive BCa patients and from 23 non-cancerous control subjects, comprising 13 patients with urolithiasis and 10 patients with unconfirmed BCa suspicion (noBCa). For the NGS analyses, a nested PCR procedure was used to amplify a TERT promoter fragment covering both mutations followed by unidirectional sequencing on an Ion Torrent PGM semiconductor-based device (Life Technologies). Additionally, a previously reported ddPCR assay was applied on the same DNA samples using the QX100 Droplet Digital PCR system (BIO-RAD). The diagnostic performance of the mutation allele frequencies (MAFs) determined by these methods was compared with the performance of VUC. RESULTS: The DNA amount needed for NGS (1-5 ng) and ddPCR (5 ng) was easily available from urinary sediments. The determined detection limit was of 0.4% for the C250T mutation and 0.75% for the C228T mutation in the NGS analyses, whereas it was 1% for both mutations in the ddPCR assay. Of the BCa patients 77.6% and 69.4% were positive for one of the two mutations in the NGS and in the ddPCR, respectively. The concordance between both techniques amounted to 93.1% (rs=0.866, p<0.001). However, the mutation recovery rate in the urinary DNA was lower in comparison to 85.7% of TERT mutation-positive BCa patients determined in the TUR B specimens. The median of the combined MAFs C228T+C250T was 27.4% (range 1.5 - 77.3%) for NGS and 20.7% (range 2.2 - 44.2%) for ddPCR. The NGS and the ddPCR assays performed better than the VUC reflected by values for sensitivity of 0.78/0.69/0.67, for specificity of 0.96/1.00/0.96, for PPV of 0.97/1.00/0.97, for NPV of 0.67/0.61/0.58 and for accuracy of 0.83/0.79/0.76. According to this the NGS assay seems to be the best approach, but it is far more expensive (50 EUR per sample) than the ddPCR assay or the VUC (max. 3 EUR per sample). CONCLUSIONS: The assessment of TERT promoter mutations by NGS and ddPCR provides a slightly better diagnostic performance than VUC but according to the costs only ddPCR might be an alternative. Source of Funding: none Dresden, Germany© 2019 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e898-e899 Advertisement Copyright & Permissions© 2019 by American Urological Association Education and Research, Inc.MetricsAuthor Information Friedrich Stoeckel More articles by this author Karsten Salomo More articles by this author Stasik Sebastian More articles by this author Christian Thiede More articles by this author Mario Menschikowski More articles by this author Ulrike Heberling More articles by this author Manfred P. Wirth More articles by this author Susanne Fuessel* More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
• W2942341256 created "2019-05-03" @default.
• W2942341256 creator A5006122560 @default.
• W2942341256 creator A5014165335 @default.
• W2942341256 creator A5018158979 @default.
• W2942341256 creator A5038397023 @default.
• W2942341256 creator A5039751847 @default.
• W2942341256 creator A5047480914 @default.
• W2942341256 creator A5065289402 @default.
• W2942341256 creator A5068928733 @default.
• W2942341256 date "2019-04-01" @default.
• W2942341256 modified "2023-09-27" @default.
• W2942341256 title "MP63-06 COMPARATIVE DETERMINATION OF TERT MUTATIONS IN URINARY SEDIMENT DNA BY NGS AND DDPCR FOR BLADDER CANCER DETECTION" @default.
• W2942341256 doi "https://doi.org/10.1097/01.ju.0000556849.64618.1d" @default.
• W2942341256 hasPublicationYear "2019" @default.
• W2942341256 type Work @default.
• W2942341256 sameAs 2942341256 @default.
• W2942341256 citedByCount "0" @default.
• W2942341256 crossrefType "journal-article" @default.
• W2942341256 hasAuthorship W2942341256A5006122560 @default.
• W2942341256 hasAuthorship W2942341256A5014165335 @default.
• W2942341256 hasAuthorship W2942341256A5018158979 @default.
• W2942341256 hasAuthorship W2942341256A5038397023 @default.
• W2942341256 hasAuthorship W2942341256A5039751847 @default.
• W2942341256 hasAuthorship W2942341256A5047480914 @default.
• W2942341256 hasAuthorship W2942341256A5065289402 @default.
• W2942341256 hasAuthorship W2942341256A5068928733 @default.
• W2942341256 hasBestOaLocation W29423412561 @default.
• W2942341256 hasConcept C104317684 @default.
• W2942341256 hasConcept C121608353 @default.
• W2942341256 hasConcept C126322002 @default.
• W2942341256 hasConcept C17757408 @default.
• W2942341256 hasConcept C2780026642 @default.
• W2942341256 hasConcept C2780352672 @default.
• W2942341256 hasConcept C2910111155 @default.
• W2942341256 hasConcept C48126324 @default.
• W2942341256 hasConcept C49105822 @default.
• W2942341256 hasConcept C51679486 @default.
• W2942341256 hasConcept C54355233 @default.
• W2942341256 hasConcept C552990157 @default.
• W2942341256 hasConcept C71924100 @default.
• W2942341256 hasConcept C77411442 @default.
• W2942341256 hasConcept C86803240 @default.
• W2942341256 hasConceptScore W2942341256C104317684 @default.
• W2942341256 hasConceptScore W2942341256C121608353 @default.
• W2942341256 hasConceptScore W2942341256C126322002 @default.
• W2942341256 hasConceptScore W2942341256C17757408 @default.
• W2942341256 hasConceptScore W2942341256C2780026642 @default.
• W2942341256 hasConceptScore W2942341256C2780352672 @default.
• W2942341256 hasConceptScore W2942341256C2910111155 @default.
• W2942341256 hasConceptScore W2942341256C48126324 @default.
• W2942341256 hasConceptScore W2942341256C49105822 @default.
• W2942341256 hasConceptScore W2942341256C51679486 @default.
• W2942341256 hasConceptScore W2942341256C54355233 @default.
• W2942341256 hasConceptScore W2942341256C552990157 @default.
• W2942341256 hasConceptScore W2942341256C71924100 @default.
• W2942341256 hasConceptScore W2942341256C77411442 @default.
• W2942341256 hasConceptScore W2942341256C86803240 @default.
• W2942341256 hasIssue "Supplement 4" @default.
• W2942341256 hasLocation W29423412561 @default.
• W2942341256 hasOpenAccess W2942341256 @default.
• W2942341256 hasPrimaryLocation W29423412561 @default.
• W2942341256 hasRelatedWork W1974919387 @default.
• W2942341256 hasRelatedWork W1991390161 @default.
• W2942341256 hasRelatedWork W2120561004 @default.
• W2942341256 hasRelatedWork W2351159130 @default.
• W2942341256 hasRelatedWork W2352586825 @default.
• W2942341256 hasRelatedWork W2354987488 @default.
• W2942341256 hasRelatedWork W2358365698 @default.
• W2942341256 hasRelatedWork W2387835647 @default.
• W2942341256 hasRelatedWork W2468051081 @default.
• W2942341256 hasRelatedWork W2728852153 @default.
• W2942341256 hasVolume "201" @default.
• W2942341256 isParatext "false" @default.
• W2942341256 isRetracted "false" @default.
• W2942341256 magId "2942341256" @default.
• W2942341256 workType "article" @default.