Matches in SemOpenAlex for { <https://semopenalex.org/work/W2942466100> ?p ?o ?g. }
- W2942466100 endingPage "594" @default.
- W2942466100 startingPage "594" @default.
- W2942466100 abstract "Pheochromocytomas and paragangliomas (PPGLs) with activated pseudohypoxic pathways are associated with an immature catecholamine phenotype and carry a higher risk for metastasis. For improved understanding of the underlying mechanisms we investigated the impact of hypoxia and pseudohypoxia on catecholamine biosynthesis in pheochromocytoma cells naturally lacking Hif2α (MPC and MTT) or expressing both Hif1α and Hif2α (PC12). Cultivation under extrinsic hypoxia or in spheroid culture (intrinsic hypoxia) increased cellular dopamine and norepinephrine contents in all cell lines. To distinguish further between Hif1α- and Hif2α-driven effects we expressed Hif2α in MTT and MPC-mCherry cells (naturally lacking Hif2α). Presence of Hif2α resulted in similarly increased cellular dopamine and norepinephrine under hypoxia as in the control cells. Furthermore, hypoxia resulted in enhanced phosphorylation of tyrosine hydroxylase (TH). A specific knockdown of Hif1α in PC12 diminished these effects. Pseudohypoxic conditions, simulated by expression of Hif2α under normoxia resulted in increased TH phosphorylation, further stimulated by extrinsic hypoxia. Correlations with PPGL tissue data led us to conclude that catecholamine biosynthesis under hypoxia is mainly mediated through increased phosphorylation of TH, regulated as a short-term response (24–48 h) by HIF1α. Continuous activation of hypoxia-related genes under pseudohypoxia leads to a HIF2α-mediated phosphorylation of TH (permanent status)." @default.
- W2942466100 created "2019-05-03" @default.
- W2942466100 creator A5014493649 @default.
- W2942466100 creator A5016501548 @default.
- W2942466100 creator A5027762147 @default.
- W2942466100 creator A5028135050 @default.
- W2942466100 creator A5029679652 @default.
- W2942466100 creator A5034345217 @default.
- W2942466100 creator A5038915725 @default.
- W2942466100 creator A5046768397 @default.
- W2942466100 creator A5051607812 @default.
- W2942466100 creator A5074211261 @default.
- W2942466100 creator A5077562986 @default.
- W2942466100 creator A5086741053 @default.
- W2942466100 creator A5089586541 @default.
- W2942466100 date "2019-04-28" @default.
- W2942466100 modified "2023-10-16" @default.
- W2942466100 title "Impact of Extrinsic and Intrinsic Hypoxia on Catecholamine Biosynthesis in Absence or Presence of Hif2α in Pheochromocytoma Cells" @default.
- W2942466100 cites W1746280027 @default.
- W2942466100 cites W1907985808 @default.
- W2942466100 cites W1975404719 @default.
- W2942466100 cites W1981005501 @default.
- W2942466100 cites W1988377666 @default.
- W2942466100 cites W1996778146 @default.
- W2942466100 cites W2010386340 @default.
- W2942466100 cites W2010469141 @default.
- W2942466100 cites W2013975403 @default.
- W2942466100 cites W2014318015 @default.
- W2942466100 cites W2034634607 @default.
- W2942466100 cites W2052125807 @default.
- W2942466100 cites W2052415197 @default.
- W2942466100 cites W2052504887 @default.
- W2942466100 cites W2052947469 @default.
- W2942466100 cites W2063687158 @default.
- W2942466100 cites W2085696636 @default.
- W2942466100 cites W2088507472 @default.
- W2942466100 cites W2089459615 @default.
- W2942466100 cites W2092001446 @default.
- W2942466100 cites W2094454542 @default.
- W2942466100 cites W2095150694 @default.
- W2942466100 cites W2096706681 @default.
- W2942466100 cites W2099532184 @default.
- W2942466100 cites W2101084498 @default.
- W2942466100 cites W2102321124 @default.
- W2942466100 cites W2102941087 @default.
- W2942466100 cites W2114431124 @default.
- W2942466100 cites W2115377381 @default.
- W2942466100 cites W2115406537 @default.
- W2942466100 cites W2119809414 @default.
- W2942466100 cites W2135779821 @default.
- W2942466100 cites W2136419196 @default.
- W2942466100 cites W2139228552 @default.
- W2942466100 cites W2140723257 @default.
- W2942466100 cites W2204695292 @default.
- W2942466100 cites W2314538372 @default.
- W2942466100 cites W2464138887 @default.
- W2942466100 cites W2585589460 @default.
- W2942466100 cites W2734036824 @default.
- W2942466100 cites W2750141640 @default.
- W2942466100 cites W2803425946 @default.
- W2942466100 cites W2884967473 @default.
- W2942466100 cites W2889267256 @default.
- W2942466100 cites W2997389686 @default.
- W2942466100 cites W37292645 @default.
- W2942466100 cites W87779505 @default.
- W2942466100 doi "https://doi.org/10.3390/cancers11050594" @default.
- W2942466100 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6562431" @default.
- W2942466100 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31035382" @default.
- W2942466100 hasPublicationYear "2019" @default.
- W2942466100 type Work @default.
- W2942466100 sameAs 2942466100 @default.
- W2942466100 citedByCount "20" @default.
- W2942466100 countsByYear W29424661002019 @default.
- W2942466100 countsByYear W29424661002020 @default.
- W2942466100 countsByYear W29424661002021 @default.
- W2942466100 countsByYear W29424661002022 @default.
- W2942466100 countsByYear W29424661002023 @default.
- W2942466100 crossrefType "journal-article" @default.
- W2942466100 hasAuthorship W2942466100A5014493649 @default.
- W2942466100 hasAuthorship W2942466100A5016501548 @default.
- W2942466100 hasAuthorship W2942466100A5027762147 @default.
- W2942466100 hasAuthorship W2942466100A5028135050 @default.
- W2942466100 hasAuthorship W2942466100A5029679652 @default.
- W2942466100 hasAuthorship W2942466100A5034345217 @default.
- W2942466100 hasAuthorship W2942466100A5038915725 @default.
- W2942466100 hasAuthorship W2942466100A5046768397 @default.
- W2942466100 hasAuthorship W2942466100A5051607812 @default.
- W2942466100 hasAuthorship W2942466100A5074211261 @default.
- W2942466100 hasAuthorship W2942466100A5077562986 @default.
- W2942466100 hasAuthorship W2942466100A5086741053 @default.
- W2942466100 hasAuthorship W2942466100A5089586541 @default.
- W2942466100 hasBestOaLocation W29424661001 @default.
- W2942466100 hasConcept C104317684 @default.
- W2942466100 hasConcept C11960822 @default.
- W2942466100 hasConcept C126322002 @default.
- W2942466100 hasConcept C134018914 @default.
- W2942466100 hasConcept C159167319 @default.
- W2942466100 hasConcept C160450060 @default.