Matches in SemOpenAlex for { <https://semopenalex.org/work/W2942534462> ?p ?o ?g. }
- W2942534462 abstract "For clinical and/or pharmaceutical use of human-induced pluripotent stem cells (hiPSCs), large cell quantities of high quality are demanded. Therefore, we combined the expansion of hiPSCs in closed, perfusion-based 3D bioreactors with noninvasive online monitoring of oxygen as culture control mechanism. Bioreactors with a cell compartment volume of 3 or 17 ml were inoculated with either 10 × 106 or 50 × 106 cells, and cells were expanded over 15 days with online oxygen and offline glucose and lactate measurements being performed. The CellTiter-Blue® Assay was performed at the end of the bioreactor experiments for indirect cell quantification. Model simulations enabled an estimation of cell numbers based on kinetic equations and experimental data during the 15-day bioreactor cultures. Calculated oxygen uptake rates (OUR), glucose consumption rates (GCR), and lactate production rates (LPR) revealed a highly significant correlation (p < 0.0001). Oxygen consumption, which was measured at the beginning and the end of the experiment, showed a strong culture growth in line with the OUR and GCR data. Furthermore, the yield coefficient of lactate from glucose and the OUR to GCR ratio revealed a shift from nonoxidative to oxidative metabolism. The presented results indicate that oxygen is equally as applicable as parameter for hiPSC expansion as glucose while providing an accurate real-time impression of hiPSC culture development. Additionally, oxygen measurements inform about the metabolic state of the cells. Thus, the use of oxygen online monitoring for culture control facilitates the translation of hiPSC use to the clinical setting." @default.
- W2942534462 created "2019-05-09" @default.
- W2942534462 creator A5005610155 @default.
- W2942534462 creator A5018466333 @default.
- W2942534462 creator A5020200044 @default.
- W2942534462 creator A5028166296 @default.
- W2942534462 creator A5038061458 @default.
- W2942534462 creator A5045805327 @default.
- W2942534462 creator A5046043238 @default.
- W2942534462 creator A5056257763 @default.
- W2942534462 creator A5078296617 @default.
- W2942534462 creator A5085075958 @default.
- W2942534462 date "2019-05-29" @default.
- W2942534462 modified "2023-10-03" @default.
- W2942534462 title "Online measurement of oxygen enables continuous noninvasive evaluation of human‐induced pluripotent stem cell ( <scp>hiPSC</scp> ) culture in a perfused 3D hollow‐fiber bioreactor" @default.
- W2942534462 cites W1480764297 @default.
- W2942534462 cites W1573438640 @default.
- W2942534462 cites W1606848128 @default.
- W2942534462 cites W1808353779 @default.
- W2942534462 cites W1970705060 @default.
- W2942534462 cites W1987783568 @default.
- W2942534462 cites W1990785657 @default.
- W2942534462 cites W1991718549 @default.
- W2942534462 cites W1995629728 @default.
- W2942534462 cites W1999434929 @default.
- W2942534462 cites W2003424598 @default.
- W2942534462 cites W2005539294 @default.
- W2942534462 cites W2011345599 @default.
- W2942534462 cites W2013564442 @default.
- W2942534462 cites W2018497025 @default.
- W2942534462 cites W2024137198 @default.
- W2942534462 cites W2025192477 @default.
- W2942534462 cites W2028237664 @default.
- W2942534462 cites W2028264366 @default.
- W2942534462 cites W2028531378 @default.
- W2942534462 cites W2028711069 @default.
- W2942534462 cites W2036075345 @default.
- W2942534462 cites W2051531582 @default.
- W2942534462 cites W2056740953 @default.
- W2942534462 cites W2059962989 @default.
- W2942534462 cites W2067959462 @default.
- W2942534462 cites W2072239613 @default.
- W2942534462 cites W2076289226 @default.
- W2942534462 cites W2080196886 @default.
- W2942534462 cites W2081635461 @default.
- W2942534462 cites W2084037323 @default.
- W2942534462 cites W2088447615 @default.
- W2942534462 cites W2100884694 @default.
- W2942534462 cites W2117891320 @default.
- W2942534462 cites W2125525850 @default.
- W2942534462 cites W2126989579 @default.
- W2942534462 cites W2168865373 @default.
- W2942534462 cites W2463534616 @default.
- W2942534462 cites W2513501421 @default.
- W2942534462 cites W2524678959 @default.
- W2942534462 cites W2566174351 @default.
- W2942534462 cites W2580933284 @default.
- W2942534462 cites W2612493242 @default.
- W2942534462 cites W2753247691 @default.
- W2942534462 cites W2762965851 @default.
- W2942534462 cites W2784050294 @default.
- W2942534462 cites W2786003901 @default.
- W2942534462 cites W2806697624 @default.
- W2942534462 cites W2808551625 @default.
- W2942534462 cites W2883854607 @default.
- W2942534462 cites W2897566824 @default.
- W2942534462 cites W2944198170 @default.
- W2942534462 doi "https://doi.org/10.1002/term.2871" @default.
- W2942534462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31034735" @default.
- W2942534462 hasPublicationYear "2019" @default.
- W2942534462 type Work @default.
- W2942534462 sameAs 2942534462 @default.
- W2942534462 citedByCount "3" @default.
- W2942534462 countsByYear W29425344622019 @default.
- W2942534462 countsByYear W29425344622022 @default.
- W2942534462 countsByYear W29425344622023 @default.
- W2942534462 crossrefType "journal-article" @default.
- W2942534462 hasAuthorship W2942534462A5005610155 @default.
- W2942534462 hasAuthorship W2942534462A5018466333 @default.
- W2942534462 hasAuthorship W2942534462A5020200044 @default.
- W2942534462 hasAuthorship W2942534462A5028166296 @default.
- W2942534462 hasAuthorship W2942534462A5038061458 @default.
- W2942534462 hasAuthorship W2942534462A5045805327 @default.
- W2942534462 hasAuthorship W2942534462A5046043238 @default.
- W2942534462 hasAuthorship W2942534462A5056257763 @default.
- W2942534462 hasAuthorship W2942534462A5078296617 @default.
- W2942534462 hasAuthorship W2942534462A5085075958 @default.
- W2942534462 hasBestOaLocation W29425344621 @default.
- W2942534462 hasConcept C104317684 @default.
- W2942534462 hasConcept C107459253 @default.
- W2942534462 hasConcept C12554922 @default.
- W2942534462 hasConcept C136229726 @default.
- W2942534462 hasConcept C145103041 @default.
- W2942534462 hasConcept C168170006 @default.
- W2942534462 hasConcept C178790620 @default.
- W2942534462 hasConcept C185592680 @default.
- W2942534462 hasConcept C540031477 @default.
- W2942534462 hasConcept C54355233 @default.
- W2942534462 hasConcept C55493867 @default.
- W2942534462 hasConcept C71924100 @default.