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- W2942550168 abstract "Author(s): Tran, Tuan M. | Advisor(s): Abate, Adam | Abstract: The ability to engineer enzymes has wide ranging applications in industry and biotechnology. Recently, methods and tools have been developed to engineer enzymes to catalyze a broad range of reactions and conditions. Despite these achievements, the task of enzyme engineering remains quite challenging due to the vast amount of possible sequences and interactions within a protein. This dissertation describes droplet microfluidic tools and methods for ultra-high-throughput screening of enzymes. By screening through variants faster and with less reagent, we increase our chances of finding improved variants. We first describe a fabrication method to make 3-D double emulsion devices in PDMS. We then describe a droplet microfluidic method for high throughput sequence-function mapping. Finally, we detail a detergent-free method to lyse cells in droplets using electroporation. By enabling us to screen enzymes at high throughput, these droplet microfluidic technologies can be a powerful tool for enzyme discovery." @default.
- W2942550168 created "2019-05-09" @default.
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- W2942550168 date "2017-01-01" @default.
- W2942550168 modified "2023-09-27" @default.
- W2942550168 title "Droplet Microfluidic Tools and Methods for Enzyme Screening" @default.
- W2942550168 hasPublicationYear "2017" @default.
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