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• W2942627937 abstract "Several anaerobic bacteria produce butyric acid, a commodity chemical with use in chemical, pharmaceutical, food and feed industries, using complex media with acetate as a co-product. Butyrate titer of various recombinant Escherichia coli did not exceed 10 g l−1 in batch fermentations in any of the media tested. A recombinant E. coli (strain LW393) that produced butyrate as the major fermentation product was constructed with genes from E. coli, Clostridium acetobutylicum and Treponema denticola. Strain LW393 produced 323 ± 6 mM (28.4 ± 0.4 g l−1) butyric acid in batch fermentations in mineral salt medium with glucose as C source at a yield of 0.37 ± 0.01 g (g glucose consumed)−1. Butyrate accounted for 90% of the total products produced by the culture. Supplementing this medium with yeast extract further increased butyric acid titer to 375 ± 4 mM. Average volumetric productivity of butyrate with xylose as C source was 0.89 ± 0.07 g l−1 h−1. The butyrate titer reported in this study is about 2.5–3-times higher than the values reported for other recombinant E. coli and this is achieved in mineral salt medium with an expectation of lower purification and production cost of butyrate." @default.
• W2942627937 created "2019-05-09" @default.
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• W2942627937 date "2019-03-22" @default.
• W2942627937 modified "2023-10-15" @default.
• W2942627937 title "Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity" @default.
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• W2942627937 doi "https://doi.org/10.1186/s13068-019-1408-9" @default.
• W2942627937 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6429758" @default.
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