Matches in SemOpenAlex for { <https://semopenalex.org/work/W2942676231> ?p ?o ?g. }
- W2942676231 abstract "Ca2+-stimulated translocation of cytosolic phospholipase A2α (cPLA2α) to the Golgi induces arachidonic acid production, the rate-limiting step in pro-inflammatory eicosanoid synthesis. Structural insights into the cPLA2α preference for phosphatidylcholine (PC)-enriched membranes have remained elusive. Here, we report the structure of the cPLA2α C2-domain (at 2.2 Å resolution), which contains bound 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) and Ca2+ ions. Two Ca2+ are complexed at previously reported locations in the lipid-free C2-domain. One of these Ca2+ions, along with a third Ca2+, bridges the C2-domain to the DHPC phosphate group, which also interacts with Asn65. Tyr96 plays a key role in lipid headgroup recognition via cation-π interaction with the PC trimethylammonium group. Mutagenesis analyses confirm that Tyr96 and Asn65 function in PC binding selectivity by the C2-domain and in the regulation of cPLA2α activity. The DHPC-binding mode of the cPLA2α C2-domain, which differs from phosphatidylserine or phosphatidylinositol 4,5-bisphosphate binding by other C2-domains, expands and deepens knowledge of the lipid-binding mechanisms mediated by C2-domains." @default.
- W2942676231 created "2019-05-09" @default.
- W2942676231 creator A5009408265 @default.
- W2942676231 creator A5022303540 @default.
- W2942676231 creator A5030397954 @default.
- W2942676231 creator A5030623342 @default.
- W2942676231 creator A5031949661 @default.
- W2942676231 creator A5067157897 @default.
- W2942676231 creator A5077706769 @default.
- W2942676231 creator A5079674683 @default.
- W2942676231 creator A5085684647 @default.
- W2942676231 date "2019-05-03" @default.
- W2942676231 modified "2023-10-17" @default.
- W2942676231 title "Structural basis of phosphatidylcholine recognition by the C2–domain of cytosolic phospholipase A2α" @default.
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