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- W2942856625 abstract "Abstract The spot capacity in thin-layer chromatography (TLC) is the number of spots resolved with a resolution of unity that can be placed between the sample spot and the spot of a non-retained compound. This is more difficult to calculate than the equivalent peak capacity in column chromatography as the plate height in TLC is a complex function of the characteristics of the solvent used and the plate, including particle size and development lenght. An iteration method is used. The results show that it is very easy to achieve a spot capacity between 10 and 20, but it is extremely difficult to reach 25 and practically impossible to exceed 30 except in very favourable circumstances. The spot capacity increases with decreasing diffusion coefficient and increasing kinetic coefficient of the solvent and plate quality (packing homogeneity). For a given solvent and development length there is an optimum particle size that provides the maximum spot capacity. The fastest separation is achieved using slightly larger particles. The spot capacity also increases with increasing development distance towards a limit." @default.
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- W2942856625 date "1982-08-01" @default.
- W2942856625 modified "2023-09-23" @default.
- W2942856625 title "Study of the performances of thin-layer chromatography" @default.
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- W2942856625 doi "https://doi.org/10.1016/s0021-9673(00)82470-x" @default.
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