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- W2942861708 abstract "Electron cryo-tomography using the scanning transmission modality (STEM) enables 3D reconstruction of unstained, vitrified specimens as thick as 1 μm or more. Contrast is related to mass/thickness and atomic number, providing quantifiable chemical characterization and mass mapping of intact prokaryotic and eukaryotic cells. Energy dispersive X-ray spectroscopy by STEM provides a simple, on-the-spot chemical identification of the elemental composition in sub-cellular organic bodies or mineral deposits. This chapter provides basic background and practical information for performing cryo-STEM tomography on vitrified biological cells." @default.
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- W2942861708 date "2019-01-01" @default.
- W2942861708 modified "2023-09-27" @default.
- W2942861708 title "CryoSTEM tomography in biology" @default.
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- W2942861708 doi "https://doi.org/10.1016/bs.mcb.2019.04.001" @default.
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