FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2942868281> ?p ?o ?g. }

• W2942868281 endingPage "100375" @default.
• W2942868281 startingPage "100375" @default.
• W2942868281 abstract "Abstract In this study, we investigated the response to heavy metals of recombinant E. coli NovaBlue strains carrying plasmids harboring different Cupriavidus taiwanensis LMG19424-derived groEL promoter fragments fused to the lacZ reporter gene. Four recombinant plasmids (designated G14, G26, G27 and G28) were transformed into E. coli and screened for the ability to indicate the presence of heavy metals on Luria–Bertani (LB) agar. Only the G28 biosensor exhibited reproducible results, with an observed limit of Cd detection of 3 μ g/mL. In LB broth, the NovaBlue host strain was able to grow and tolerate in the presence of 50 μ g/mL As, Cd, Co, Ni, Pb and Zn but was susceptible to 6.2 μ g/mL Cr. The β -galactosidase ( β -gal) reporter activity was reduced when the concentration of heavy metals increased, with observed detection limits, as indicated by the blue color, of 25 μ g/mL for CdCl2, CdSO4, and CdCO3; 50 μ g/mL for As (III) and ZnSO4; and 100 μ g/mL for CoCl2 and NiSO4. The specificity of the induction ratio and metal concentration was highest for Cd, with R2 values of 0.9196 and 0.9164 for CdCl2 and CdSO4, respectively. When the ratio of β -gal activity and growth was compared between NovaBlue strains carrying G28 or pETBlue-2 (containing an E. coli promoter), the G28 biosensor exhibited a significantly higher ratio than the strain carrying pETBlue-2 at 0.8–6.3 μ g/mL CdCl2. The results demonstrated that the certain concentration range corresponded to Cd induction of G28. The G28 biosensor were immobilized on paper discs and tested using the most toxic and soluble Cd compound (CdCl2) at 0–0.8 μ g/mL on LB agar. The G28 biosensor developed a blue color only in the absence of CdCl2. However, when testing soil extracts contaminated with low, medium or high levels of total Cd or those spiked with CdCl2 (up to 40 μ g/mL), all the G28 biosensor discs showed a similar growth pattern with a spotted blue color compared with the even blue color of the control disc. Due to the complexity of environmental samples, several factors may interfere with the metal response of the G28 biosensor. However, the G28 biosensor exhibited different behaviors in the presence of specific types and concentrations of metals. Thus, combinations of G28 and more specific promoters should be further investigated to improve the sensitivity and specificity of the groEL promoter for detecting Cd and other heavy metals in contaminated samples." @default.
• W2942868281 created "2019-05-09" @default.
• W2942868281 creator A5030699201 @default.
• W2942868281 creator A5044655299 @default.
• W2942868281 creator A5046779417 @default.
• W2942868281 creator A5080893920 @default.
• W2942868281 date "2019-08-01" @default.
• W2942868281 modified "2023-09-25" @default.
• W2942868281 title "Potential application of a recombinant bacterial strain carrying a groEL promoter as a whole-cell microbial biosensor for detecting bioavailable cadmium" @default.
• W2942868281 cites W1970155775 @default.
• W2942868281 cites W1979519782 @default.
• W2942868281 cites W1980112837 @default.
• W2942868281 cites W1983134170 @default.
• W2942868281 cites W1987545559 @default.
• W2942868281 cites W1989134100 @default.
• W2942868281 cites W2002714080 @default.
• W2942868281 cites W2022772009 @default.
• W2942868281 cites W2034375018 @default.
• W2942868281 cites W2038008759 @default.
• W2942868281 cites W2064563385 @default.
• W2942868281 cites W2070957092 @default.
• W2942868281 cites W2075755324 @default.
• W2942868281 cites W2076502427 @default.
• W2942868281 cites W2088301235 @default.
• W2942868281 cites W2106524085 @default.
• W2942868281 cites W2117166117 @default.
• W2942868281 cites W2144379774 @default.
• W2942868281 cites W2147003905 @default.
• W2942868281 cites W2153276014 @default.
• W2942868281 cites W2161760101 @default.
• W2942868281 cites W2266289900 @default.
• W2942868281 cites W230921398 @default.
• W2942868281 cites W2319829705 @default.
• W2942868281 cites W2328661854 @default.
• W2942868281 cites W2462589184 @default.
• W2942868281 cites W2532337268 @default.
• W2942868281 cites W266235207 @default.
• W2942868281 cites W266695354 @default.
• W2942868281 cites W2766641990 @default.
• W2942868281 cites W2899685399 @default.
• W2942868281 cites W644740770 @default.
• W2942868281 doi "https://doi.org/10.1016/j.eti.2019.100375" @default.
• W2942868281 hasPublicationYear "2019" @default.
• W2942868281 type Work @default.
• W2942868281 sameAs 2942868281 @default.
• W2942868281 citedByCount "6" @default.
• W2942868281 countsByYear W29428682812020 @default.
• W2942868281 countsByYear W29428682812021 @default.
• W2942868281 countsByYear W29428682812022 @default.
• W2942868281 crossrefType "journal-article" @default.
• W2942868281 hasAuthorship W2942868281A5030699201 @default.
• W2942868281 hasAuthorship W2942868281A5044655299 @default.
• W2942868281 hasAuthorship W2942868281A5046779417 @default.
• W2942868281 hasAuthorship W2942868281A5080893920 @default.
• W2942868281 hasConcept C104317684 @default.
• W2942868281 hasConcept C105702510 @default.
• W2942868281 hasConcept C160756335 @default.
• W2942868281 hasConcept C178790620 @default.
• W2942868281 hasConcept C181389837 @default.
• W2942868281 hasConcept C185592680 @default.
• W2942868281 hasConcept C2778022156 @default.
• W2942868281 hasConcept C40767141 @default.
• W2942868281 hasConcept C523546767 @default.
• W2942868281 hasConcept C54355233 @default.
• W2942868281 hasConcept C544657597 @default.
• W2942868281 hasConcept C547475151 @default.
• W2942868281 hasConcept C55493867 @default.
• W2942868281 hasConcept C60644358 @default.
• W2942868281 hasConcept C86803240 @default.
• W2942868281 hasConcept C87190427 @default.
• W2942868281 hasConcept C89423630 @default.
• W2942868281 hasConceptScore W2942868281C104317684 @default.
• W2942868281 hasConceptScore W2942868281C105702510 @default.
• W2942868281 hasConceptScore W2942868281C160756335 @default.
• W2942868281 hasConceptScore W2942868281C178790620 @default.
• W2942868281 hasConceptScore W2942868281C181389837 @default.
• W2942868281 hasConceptScore W2942868281C185592680 @default.
• W2942868281 hasConceptScore W2942868281C2778022156 @default.
• W2942868281 hasConceptScore W2942868281C40767141 @default.
• W2942868281 hasConceptScore W2942868281C523546767 @default.
• W2942868281 hasConceptScore W2942868281C54355233 @default.
• W2942868281 hasConceptScore W2942868281C544657597 @default.
• W2942868281 hasConceptScore W2942868281C547475151 @default.
• W2942868281 hasConceptScore W2942868281C55493867 @default.
• W2942868281 hasConceptScore W2942868281C60644358 @default.
• W2942868281 hasConceptScore W2942868281C86803240 @default.
• W2942868281 hasConceptScore W2942868281C87190427 @default.
• W2942868281 hasConceptScore W2942868281C89423630 @default.
• W2942868281 hasLocation W29428682811 @default.
• W2942868281 hasOpenAccess W2942868281 @default.
• W2942868281 hasPrimaryLocation W29428682811 @default.
• W2942868281 hasRelatedWork W1618810518 @default.
• W2942868281 hasRelatedWork W1971071241 @default.
• W2942868281 hasRelatedWork W1972445536 @default.
• W2942868281 hasRelatedWork W1993235233 @default.
• W2942868281 hasRelatedWork W2367337584 @default.
• W2942868281 hasRelatedWork W2368672388 @default.
• W2942868281 hasRelatedWork W2381767722 @default.

• next