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- W2942908572 startingPage "624890" @default.
- W2942908572 abstract "Abstract Single-cell RNA-seq has emerged as a powerful method to unravel heterogeneity of complex biological systems; this has enabled in vivo characterization of cell type compositions through unsupervised sampling and modelling of transcriptional states in single cells. Here we used the high-throughput microfluidic-based single-cell RNA-seq method Drop-seq to elucidate the cellular composition and functional diversity of breast tumours during the induction of metastatic disease in a transgenic model related to pregnancy-associated breast cancer (PABC). We characterised with unprecedented definition, how the activation of developmental programs associated to pregnancy results in the acquisition of an aggressive phenotype. We show that cancer cells are classified in a structure comparable with the lineages of the epithelial mammary gland hierarchy, revealing high dynamics and plasticity of cancer cells during disease progression. This cancer progression program is orchestrated by alveolar milk secretory cells, that in conjunction with cell types from the tumour microenvironment (TME), including cancer-associated fibroblasts (CAFs), form a multi-cellular process that resembles an aberrant involution. Finally, we analysed the interactome of the tumour ecosystem to define a high-resolution landscape of the molecular pathways of cell-to-cell communication that underpins extra-cellular remodelling and inflammation associated with the aggressive involution mimicry. In conclusion, our study recapitulates developmental mechanisms that have gone awry during carcinogenesis in a model of PABC. We provide a large-scale single-cell transcriptional landscape of breast tumours that allows unprecedented understanding of breast heterogeneity and deep analysis of key molecular events that result in cancer malignancy." @default.
- W2942908572 created "2019-05-09" @default.
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- W2942908572 date "2019-05-03" @default.
- W2942908572 modified "2023-09-26" @default.
- W2942908572 title "Single-cell RNAseq uncovers involution mimicry as an aberrant development pathway during breast cancer metastasis" @default.
- W2942908572 cites W102699897 @default.
- W2942908572 cites W1537272407 @default.
- W2942908572 cites W154306038 @default.
- W2942908572 cites W1582847607 @default.
- W2942908572 cites W1601214065 @default.
- W2942908572 cites W1661462671 @default.
- W2942908572 cites W1828930947 @default.
- W2942908572 cites W1849780187 @default.
- W2942908572 cites W1902156758 @default.
- W2942908572 cites W1908864745 @default.
- W2942908572 cites W1909250455 @default.
- W2942908572 cites W1964765567 @default.
- W2942908572 cites W1967891780 @default.
- W2942908572 cites W1979440527 @default.
- W2942908572 cites W1984433201 @default.
- W2942908572 cites W1984883254 @default.
- W2942908572 cites W1985995289 @default.
- W2942908572 cites W1998051987 @default.
- W2942908572 cites W1998292611 @default.
- W2942908572 cites W1999591980 @default.
- W2942908572 cites W2000067978 @default.
- W2942908572 cites W2005012247 @default.
- W2942908572 cites W2005895632 @default.
- W2942908572 cites W2010867675 @default.
- W2942908572 cites W2013868151 @default.
- W2942908572 cites W2019171053 @default.
- W2942908572 cites W2023398724 @default.
- W2942908572 cites W2028326666 @default.
- W2942908572 cites W2031941215 @default.
- W2942908572 cites W2033077654 @default.
- W2942908572 cites W2044261540 @default.
- W2942908572 cites W2044563744 @default.
- W2942908572 cites W2051020739 @default.
- W2942908572 cites W2051147675 @default.
- W2942908572 cites W2053682792 @default.
- W2942908572 cites W2059496526 @default.
- W2942908572 cites W2060530444 @default.
- W2942908572 cites W2070682624 @default.
- W2942908572 cites W2073302269 @default.
- W2942908572 cites W2076891934 @default.
- W2942908572 cites W2077986050 @default.
- W2942908572 cites W2078322485 @default.
- W2942908572 cites W2082161467 @default.
- W2942908572 cites W2085621906 @default.
- W2942908572 cites W2089217090 @default.
- W2942908572 cites W2089258206 @default.
- W2942908572 cites W2089361444 @default.
- W2942908572 cites W2090203203 @default.
- W2942908572 cites W2093283052 @default.
- W2942908572 cites W2096283457 @default.
- W2942908572 cites W2097466647 @default.
- W2942908572 cites W2102212449 @default.
- W2942908572 cites W2102689006 @default.
- W2942908572 cites W2106292881 @default.
- W2942908572 cites W2109992446 @default.
- W2942908572 cites W2111449140 @default.
- W2942908572 cites W2112793131 @default.
- W2942908572 cites W2117963341 @default.
- W2942908572 cites W2120914029 @default.
- W2942908572 cites W2122615310 @default.
- W2942908572 cites W2122855814 @default.
- W2942908572 cites W2123106337 @default.
- W2942908572 cites W2126419817 @default.
- W2942908572 cites W2128517115 @default.
- W2942908572 cites W2130410032 @default.
- W2942908572 cites W2135997472 @default.
- W2942908572 cites W2137162822 @default.
- W2942908572 cites W2148227665 @default.
- W2942908572 cites W2150493124 @default.
- W2942908572 cites W2155088648 @default.
- W2942908572 cites W2156624320 @default.
- W2942908572 cites W2159610509 @default.
- W2942908572 cites W2159707944 @default.
- W2942908572 cites W2160779973 @default.
- W2942908572 cites W2171581347 @default.
- W2942908572 cites W2212791129 @default.
- W2942908572 cites W2332292689 @default.
- W2942908572 cites W2339010719 @default.