Matches in SemOpenAlex for { <https://semopenalex.org/work/W2943062257> ?p ?o ?g. }
Showing items 1 to 47 of
47
with 100 items per page.
- W2943062257 abstract "The cupuassu tree (Theobroma grandiflorum) is an Amazonian fruit species, whose production is highly affected by the fungus Moniliophthora perniciosa, which causes the witches? broom (WB) disease. Several genes potentially involved in the response to the pathogen have been identified in cupuassu, including some that code for PR (pathogenesis related)-like proteins. In this work, the gene TgPRa was selected for functional studies by heterologous expression in tomato Micro-Tom-Rg1 (MT-Rg1). This plant presents compatible interaction with M. perniciosa (biotype S), being a good model for WB studies. The TgPRa gene was cloned into pBI121, under the control of the Arabidopsis ACT2 promoter. The resulting vector, pBI121-TgPRa, also containing the gus reporter gene and the nptII selection gene, was inserted into Agrobacterium tumefaciens GV3101, which was used in the MT-Rg1 transformation. Kanamycin resistant and GUS-positive T0 plants, potentially transformed with the gene of interest, were obtained, what will be confirmed by PCR and Southern blot analysis. Plants are being grown to obtain MT-Rg1 homozygous for TgPRa. These plants will, in near future, be submitted to bioassays with M. perniciosa and other phytopathogenic fungi, aiming to evaluate the role of TgPRa in the development of WB, as well as other diseases, including some of interest to tomato cultivation." @default.
- W2943062257 created "2019-05-09" @default.
- W2943062257 creator A5003043432 @default.
- W2943062257 creator A5015959585 @default.
- W2943062257 creator A5023473143 @default.
- W2943062257 creator A5058618112 @default.
- W2943062257 creator A5070071569 @default.
- W2943062257 date "2018-01-01" @default.
- W2943062257 modified "2023-09-24" @default.
- W2943062257 title "Transformação de tomate Micro-Tom com um gene tipo-PR de cupuaçuzeiro (Theobroma grandiflorum) visando o estudo de função da proteína." @default.
- W2943062257 hasPublicationYear "2018" @default.
- W2943062257 type Work @default.
- W2943062257 sameAs 2943062257 @default.
- W2943062257 citedByCount "0" @default.
- W2943062257 crossrefType "journal-article" @default.
- W2943062257 hasAuthorship W2943062257A5003043432 @default.
- W2943062257 hasAuthorship W2943062257A5015959585 @default.
- W2943062257 hasAuthorship W2943062257A5023473143 @default.
- W2943062257 hasAuthorship W2943062257A5058618112 @default.
- W2943062257 hasAuthorship W2943062257A5070071569 @default.
- W2943062257 hasConcept C104317684 @default.
- W2943062257 hasConcept C143065580 @default.
- W2943062257 hasConcept C204241405 @default.
- W2943062257 hasConcept C2776162087 @default.
- W2943062257 hasConcept C2779491563 @default.
- W2943062257 hasConcept C2779656422 @default.
- W2943062257 hasConcept C2780816236 @default.
- W2943062257 hasConcept C54355233 @default.
- W2943062257 hasConcept C59822182 @default.
- W2943062257 hasConcept C86803240 @default.
- W2943062257 hasConceptScore W2943062257C104317684 @default.
- W2943062257 hasConceptScore W2943062257C143065580 @default.
- W2943062257 hasConceptScore W2943062257C204241405 @default.
- W2943062257 hasConceptScore W2943062257C2776162087 @default.
- W2943062257 hasConceptScore W2943062257C2779491563 @default.
- W2943062257 hasConceptScore W2943062257C2779656422 @default.
- W2943062257 hasConceptScore W2943062257C2780816236 @default.
- W2943062257 hasConceptScore W2943062257C54355233 @default.
- W2943062257 hasConceptScore W2943062257C59822182 @default.
- W2943062257 hasConceptScore W2943062257C86803240 @default.
- W2943062257 hasLocation W29430622571 @default.
- W2943062257 hasOpenAccess W2943062257 @default.
- W2943062257 hasPrimaryLocation W29430622571 @default.
- W2943062257 isParatext "false" @default.
- W2943062257 isRetracted "false" @default.
- W2943062257 magId "2943062257" @default.
- W2943062257 workType "article" @default.