Matches in SemOpenAlex for { <https://semopenalex.org/work/W2943773301> ?p ?o ?g. }
- W2943773301 endingPage "2265" @default.
- W2943773301 startingPage "2248" @default.
- W2943773301 abstract "Apolipoprotein E4 (ApoE4) is one of three (E2, E3 and E4) human isoforms of an α-helical, 299-amino-acid protein. Homozygosity for the ε4 allele is the major genetic risk factor for developing late-onset Alzheimer's disease (AD). ApoE2, ApoE3 and ApoE4 differ at amino acid positions 112 and 158, and these sequence variations may confer conformational differences that underlie their participation in the risk of developing AD. Here, we compared the shape, oligomerization state, conformation and stability of ApoE isoforms using a range of complementary biophysical methods including small-angle x-ray scattering, analytical ultracentrifugation, circular dichroism, x-ray fiber diffraction and transmission electron microscopy We provide an in-depth and definitive study demonstrating that all three proteins are similar in stability and conformation. However, we show that ApoE4 has a propensity to polymerize to form wavy filaments, which do not share the characteristics of cross-β amyloid fibrils. Moreover, we provide evidence for the inhibition of ApoE4 fibril formation by ApoE3. This study shows that recombinant ApoE isoforms show no significant differences at the structural or conformational level. However, self-assembly of the ApoE4 isoform may play a role in pathogenesis, and these results open opportunities for uncovering new triggers for AD onset." @default.
- W2943773301 created "2019-05-09" @default.
- W2943773301 creator A5014392546 @default.
- W2943773301 creator A5027021273 @default.
- W2943773301 creator A5032881425 @default.
- W2943773301 creator A5038274735 @default.
- W2943773301 creator A5043786818 @default.
- W2943773301 creator A5068851610 @default.
- W2943773301 creator A5089164878 @default.
- W2943773301 date "2019-05-01" @default.
- W2943773301 modified "2023-10-09" @default.
- W2943773301 title "The Molecular Basis for Apolipoprotein E4 as the Major Risk Factor for Late-Onset Alzheimer's Disease" @default.
- W2943773301 cites W111710589 @default.
- W2943773301 cites W1529259401 @default.
- W2943773301 cites W1588296570 @default.
- W2943773301 cites W1602655991 @default.
- W2943773301 cites W1736154854 @default.
- W2943773301 cites W1920171370 @default.
- W2943773301 cites W1973065853 @default.
- W2943773301 cites W1975317507 @default.
- W2943773301 cites W1976288860 @default.
- W2943773301 cites W1976725721 @default.
- W2943773301 cites W1978423478 @default.
- W2943773301 cites W1985255315 @default.
- W2943773301 cites W1988478867 @default.
- W2943773301 cites W1989270637 @default.
- W2943773301 cites W1990016625 @default.
- W2943773301 cites W1993453166 @default.
- W2943773301 cites W2001641653 @default.
- W2943773301 cites W2001688761 @default.
- W2943773301 cites W2004242436 @default.
- W2943773301 cites W2004262276 @default.
- W2943773301 cites W2004863845 @default.
- W2943773301 cites W2006534012 @default.
- W2943773301 cites W2012436507 @default.
- W2943773301 cites W2014897304 @default.
- W2943773301 cites W2015094510 @default.
- W2943773301 cites W2016579573 @default.
- W2943773301 cites W2017918965 @default.
- W2943773301 cites W2020180796 @default.
- W2943773301 cites W2026727321 @default.
- W2943773301 cites W2034945178 @default.
- W2943773301 cites W2036288989 @default.
- W2943773301 cites W2038197478 @default.
- W2943773301 cites W2038399240 @default.
- W2943773301 cites W2048287349 @default.
- W2943773301 cites W2051765035 @default.
- W2943773301 cites W2056031706 @default.
- W2943773301 cites W2056145534 @default.
- W2943773301 cites W2056516296 @default.
- W2943773301 cites W2059995793 @default.
- W2943773301 cites W2061916198 @default.
- W2943773301 cites W2062929870 @default.
- W2943773301 cites W2064179601 @default.
- W2943773301 cites W2066598200 @default.
- W2943773301 cites W2072682191 @default.
- W2943773301 cites W2074713226 @default.
- W2943773301 cites W2077434930 @default.
- W2943773301 cites W2087532566 @default.
- W2943773301 cites W2091924214 @default.
- W2943773301 cites W2094077114 @default.
- W2943773301 cites W2096693266 @default.
- W2943773301 cites W2107677733 @default.
- W2943773301 cites W2111135465 @default.
- W2943773301 cites W2115537581 @default.
- W2943773301 cites W2117156629 @default.
- W2943773301 cites W2138992210 @default.
- W2943773301 cites W2140260269 @default.
- W2943773301 cites W2154721456 @default.
- W2943773301 cites W2171346860 @default.
- W2943773301 cites W2316885128 @default.
- W2943773301 cites W2486642379 @default.
- W2943773301 cites W2618913695 @default.
- W2943773301 cites W2756959977 @default.
- W2943773301 cites W2794553092 @default.
- W2943773301 cites W2798230823 @default.
- W2943773301 cites W2883974615 @default.
- W2943773301 cites W2911991085 @default.
- W2943773301 cites W2915521488 @default.
- W2943773301 cites W2951261678 @default.
- W2943773301 cites W2996448885 @default.
- W2943773301 cites W4205945069 @default.
- W2943773301 cites W56461897 @default.
- W2943773301 doi "https://doi.org/10.1016/j.jmb.2019.04.019" @default.
- W2943773301 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6556554" @default.
- W2943773301 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31051176" @default.
- W2943773301 hasPublicationYear "2019" @default.
- W2943773301 type Work @default.
- W2943773301 sameAs 2943773301 @default.
- W2943773301 citedByCount "27" @default.
- W2943773301 countsByYear W29437733012019 @default.
- W2943773301 countsByYear W29437733012020 @default.
- W2943773301 countsByYear W29437733012021 @default.
- W2943773301 countsByYear W29437733012022 @default.
- W2943773301 countsByYear W29437733012023 @default.
- W2943773301 crossrefType "journal-article" @default.
- W2943773301 hasAuthorship W2943773301A5014392546 @default.
- W2943773301 hasAuthorship W2943773301A5027021273 @default.