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- W2943896313 abstract "Classical swine fever (CSF) is an economically important viral disease of domesticated, feral, and wild pigs. The main objective of this study is to assess the suitability of a transposon based approach for a vaccine development against CSF virus. Therefore the DNA transposon system, Sleeping Beauty (SB), has been exploited to design and develop transposon constructs expressing E2 glycoproteins of CSF virus. A codon optimized synthetic E2 and a wild type E2 gene were cloned in SB transposon vectors for the expression of membrane anchored and secretory variants. It is anticipated that the delivery with the SB system will lead to efficient integration of the E2 transposons in transcriptionally permissive loci, which will result in robust and long lasting expression. To test this concept in vivo, I characterized transgenic animals with mammary-gland restricted E2 gene expression, or ubiquitous expression of reporter constructs derived via SB transposition. A mouse line with mammary gland-specific expression of E2 gene was established, which showed stable expression of the viral E2 glycoprotein in the milk. Some of the offspring showed a dwarf phenotype, however the dwarf phenotype correlated with the integration sites and not to the E2 protein containing milk. The suitability of the SB transposon system for the identification of transcriptionally permissive loci was shown in transgenic pigs. In addition, the large scale expression of reporter transposons in the milk of transgenic pig was established. It is anticipated that the SB transposon approach via application as a DNA vaccine or via the recombinant expression of CSFV subunits in the milk of transgenic animals will contribute to improved approaches against the virus." @default.
- W2943896313 created "2019-05-16" @default.
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- W2943896313 date "2016-06-17" @default.
- W2943896313 modified "2023-09-23" @default.
- W2943896313 title "Approaches for the development of a transposon based vaccine against classical swine fever virus" @default.
- W2943896313 hasPublicationYear "2016" @default.
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