Matches in SemOpenAlex for { <https://semopenalex.org/work/W2943907257> ?p ?o ?g. }
- W2943907257 endingPage "548" @default.
- W2943907257 startingPage "538" @default.
- W2943907257 abstract "Abstract For years, extensive efforts have been made to use mammalian sperm as the mediator to generate genetically modified animals; however, the strategy of sperm-mediated gene transfer (SMGT) is unable to produce stable and diversified modifications in descendants. Recently, haploid embryonic stem cells (haESCs) have been successfully derived from haploid embryos carrying the genome of highly specialized gametes, and can stably maintain haploidy (through periodic cell sorting based on DNA quantity) and both self-renewal and pluripotency in long-term cell culture. In particular, haESCs derived from androgenetic haploid blastocysts (AG-haESCs), carrying only the sperm genome, can support the generation of live mice (semi-cloned, SC mice) through oocyte injection. Remarkably, after removal of the imprinted control regions H19-DMR (differentially methylated region of DNA) and IG-DMR in AG-haESCs, the double knockout (DKO)-AG-haESCs can stably produce SC animals with high efficiency, and so can serve as a sperm equivalent. Importantly, DKO-AG-haESCs can be used for multiple rounds of gene modifications in vitro, followed by efficient generation of live and fertile mice with the expected genetic traits. Thus, DKO-AG-haESCs (referred to as ‘artificial spermatids’) combed with CRISPR-Cas technology can be used as the genetically tractable fertilization agent, to efficiently create genetically modified offspring, and is a versatile genetic tool for in vivo analyses of gene function." @default.
- W2943907257 created "2019-05-16" @default.
- W2943907257 creator A5001977549 @default.
- W2943907257 creator A5004498551 @default.
- W2943907257 date "2019-05-11" @default.
- W2943907257 modified "2023-10-16" @default.
- W2943907257 title "‘Artificial spermatid’-mediated genome editing†" @default.
- W2943907257 cites W1416422246 @default.
- W2943907257 cites W1572202589 @default.
- W2943907257 cites W1664431272 @default.
- W2943907257 cites W1963877220 @default.
- W2943907257 cites W1964262120 @default.
- W2943907257 cites W1965707365 @default.
- W2943907257 cites W1968400039 @default.
- W2943907257 cites W1973842956 @default.
- W2943907257 cites W1973879730 @default.
- W2943907257 cites W1974901081 @default.
- W2943907257 cites W1977107900 @default.
- W2943907257 cites W1978386737 @default.
- W2943907257 cites W1980991473 @default.
- W2943907257 cites W1983350119 @default.
- W2943907257 cites W1983587836 @default.
- W2943907257 cites W1984548785 @default.
- W2943907257 cites W1989293984 @default.
- W2943907257 cites W1990895545 @default.
- W2943907257 cites W1997372839 @default.
- W2943907257 cites W1998525496 @default.
- W2943907257 cites W2003787610 @default.
- W2943907257 cites W2003990216 @default.
- W2943907257 cites W2004485432 @default.
- W2943907257 cites W2005942028 @default.
- W2943907257 cites W2006141070 @default.
- W2943907257 cites W2006487962 @default.
- W2943907257 cites W2011108720 @default.
- W2943907257 cites W2019845283 @default.
- W2943907257 cites W2021749433 @default.
- W2943907257 cites W2022518804 @default.
- W2943907257 cites W2022522122 @default.
- W2943907257 cites W2022710905 @default.
- W2943907257 cites W2023527508 @default.
- W2943907257 cites W2029568931 @default.
- W2943907257 cites W2029971236 @default.
- W2943907257 cites W2036390459 @default.
- W2943907257 cites W2045899482 @default.
- W2943907257 cites W2048681080 @default.
- W2943907257 cites W2051068182 @default.
- W2943907257 cites W2051921610 @default.
- W2943907257 cites W2053997077 @default.
- W2943907257 cites W2058792860 @default.
- W2943907257 cites W2063052421 @default.
- W2943907257 cites W2066636198 @default.
- W2943907257 cites W2066649243 @default.
- W2943907257 cites W2066741833 @default.
- W2943907257 cites W2068538911 @default.
- W2943907257 cites W2070777085 @default.
- W2943907257 cites W2074953772 @default.
- W2943907257 cites W2076469310 @default.
- W2943907257 cites W2077156813 @default.
- W2943907257 cites W2078135041 @default.
- W2943907257 cites W2079783863 @default.
- W2943907257 cites W2081651784 @default.
- W2943907257 cites W2086943348 @default.
- W2943907257 cites W2089741943 @default.
- W2943907257 cites W2089929813 @default.
- W2943907257 cites W2096811107 @default.
- W2943907257 cites W2098612555 @default.
- W2943907257 cites W2101749257 @default.
- W2943907257 cites W2110841199 @default.
- W2943907257 cites W2110951885 @default.
- W2943907257 cites W2114210098 @default.
- W2943907257 cites W2117446594 @default.
- W2943907257 cites W2122998222 @default.
- W2943907257 cites W2126212999 @default.
- W2943907257 cites W2127862779 @default.
- W2943907257 cites W2127898562 @default.
- W2943907257 cites W2129305656 @default.
- W2943907257 cites W2131395804 @default.
- W2943907257 cites W2132480780 @default.
- W2943907257 cites W2139709723 @default.
- W2943907257 cites W2140754284 @default.
- W2943907257 cites W2141677027 @default.
- W2943907257 cites W2143381146 @default.
- W2943907257 cites W2144664559 @default.
- W2943907257 cites W2146926782 @default.
- W2943907257 cites W2149236471 @default.
- W2943907257 cites W2152505144 @default.
- W2943907257 cites W2156446510 @default.
- W2943907257 cites W2158254879 @default.
- W2943907257 cites W2160064083 @default.
- W2943907257 cites W2162620349 @default.
- W2943907257 cites W2162927194 @default.
- W2943907257 cites W2163971374 @default.
- W2943907257 cites W2164259654 @default.
- W2943907257 cites W2165869860 @default.
- W2943907257 cites W2165996867 @default.
- W2943907257 cites W2167093496 @default.
- W2943907257 cites W2170039745 @default.
- W2943907257 cites W2172080201 @default.