Matches in SemOpenAlex for { <https://semopenalex.org/work/W2943940331> ?p ?o ?g. }
- W2943940331 endingPage "1987" @default.
- W2943940331 startingPage "1987" @default.
- W2943940331 abstract "Purpose: To investigate the potential neuroprotective effect of sildenafil on the ocular circulation in mice with/without optic nerve crush (ONC). Methods: Male adult mice (n = 63) were treated with intravitreal (IVT) sildenafil 24 μg/3 μL, intraperitoneal (IP) sildenafil 24 μg/300 μL, or IP saline immediately before right ONC induction (ONC group). A second group (n = 123) received the same treatments without ONC induction (naïve group). Evaluations included fluorescein angiography (naïve group; day 0), molecular studies (days 1 and 3), and retinal and optic nerve histology (day 21). Results: Maximal retinal vessel dilatation and increased choroidal effusion were detected within 30 minutes of sildenafil injection. In the ONC group, moderate retinal ganglion cell (RGC) loss was noted at 21 days. However, molecular studies showed increased stress induced gene expression (IP superoxide dismutase [SOD]-1: 3.1-fold; heme oxygenase [HO]-1: 5.8-fold; IVT SOD-1: 1.47-fold), proapoptotic gene expression (IP BAX/B-cell lymphoma [BCL]-2 10.8-/2.3-fold), and glial gene expression (IP glial fibrillary acidic protein [GFAP]: 2.8- and myelin basic protein [MBP]: 2.5-fold). In the naïve group, IVT sildenafil was not associated with RGC loss or optic nerve stroke on histology, although in two samples, molecular parameters were compatible with stroke, showing increased gene expression of HO-1 (3.8-fold) and BCL-2 (2.5-fold). In the IP sildenafil subgroup, optic neuropathy was observed in 6/120 optic nerves, including 3 cyan fluorescence protein (CFP)-Thy-1 mice. Levels of antiapoptosis and anti-ischemia genes were decreased (<0.5-fold) except for three outliers. Conclusions: Sildenafil affects retinal and choroidal perfusion in mice. When injected immediately before ONC, molecular parameters showed a preconditioning neuroprotective effect while histologic studies did not. In the absence of ONC, it is associated with neuropathy, possibly dose-dependent." @default.
- W2943940331 created "2019-05-16" @default.
- W2943940331 creator A5024034797 @default.
- W2943940331 creator A5049624036 @default.
- W2943940331 creator A5051093827 @default.
- W2943940331 creator A5053644078 @default.
- W2943940331 creator A5062349053 @default.
- W2943940331 creator A5066953827 @default.
- W2943940331 creator A5070156610 @default.
- W2943940331 creator A5076318490 @default.
- W2943940331 date "2019-05-07" @default.
- W2943940331 modified "2023-09-23" @default.
- W2943940331 title "Ocular Effects of Sildenafil in Naïve Mice and a Mouse Model of Optic Nerve Crush" @default.
- W2943940331 cites W1502088380 @default.
- W2943940331 cites W1515739182 @default.
- W2943940331 cites W1552308041 @default.
- W2943940331 cites W1577333723 @default.
- W2943940331 cites W1593006793 @default.
- W2943940331 cites W1943597576 @default.
- W2943940331 cites W1964184415 @default.
- W2943940331 cites W1972253066 @default.
- W2943940331 cites W1975068317 @default.
- W2943940331 cites W1980542990 @default.
- W2943940331 cites W1982765342 @default.
- W2943940331 cites W2008585154 @default.
- W2943940331 cites W2019044737 @default.
- W2943940331 cites W2025129645 @default.
- W2943940331 cites W2025789164 @default.
- W2943940331 cites W2026959695 @default.
- W2943940331 cites W2027202120 @default.
- W2943940331 cites W2028133364 @default.
- W2943940331 cites W2028701630 @default.
- W2943940331 cites W2029429677 @default.
- W2943940331 cites W2031522729 @default.
- W2943940331 cites W2040327798 @default.
- W2943940331 cites W2041070057 @default.
- W2943940331 cites W2041816586 @default.
- W2943940331 cites W2045762806 @default.
- W2943940331 cites W2045766392 @default.
- W2943940331 cites W2048249712 @default.
- W2943940331 cites W2050098751 @default.
- W2943940331 cites W2056055192 @default.
- W2943940331 cites W2058173935 @default.
- W2943940331 cites W2060691366 @default.
- W2943940331 cites W2068894579 @default.
- W2943940331 cites W2068981061 @default.
- W2943940331 cites W2070769478 @default.
- W2943940331 cites W2075065959 @default.
- W2943940331 cites W2093604529 @default.
- W2943940331 cites W2104664979 @default.
- W2943940331 cites W2105711200 @default.
- W2943940331 cites W2106052836 @default.
- W2943940331 cites W2119421595 @default.
- W2943940331 cites W2120001424 @default.
- W2943940331 cites W2124627119 @default.
- W2943940331 cites W2135832454 @default.
- W2943940331 cites W2136084480 @default.
- W2943940331 cites W2141627140 @default.
- W2943940331 cites W2144938268 @default.
- W2943940331 cites W2166257747 @default.
- W2943940331 cites W2416198422 @default.
- W2943940331 cites W2540737237 @default.
- W2943940331 cites W2550417267 @default.
- W2943940331 cites W2684375425 @default.
- W2943940331 cites W2737169234 @default.
- W2943940331 doi "https://doi.org/10.1167/iovs.18-26333" @default.
- W2943940331 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31063183" @default.
- W2943940331 hasPublicationYear "2019" @default.
- W2943940331 type Work @default.
- W2943940331 sameAs 2943940331 @default.
- W2943940331 citedByCount "8" @default.
- W2943940331 countsByYear W29439403312020 @default.
- W2943940331 countsByYear W29439403312022 @default.
- W2943940331 countsByYear W29439403312023 @default.
- W2943940331 crossrefType "journal-article" @default.
- W2943940331 hasAuthorship W2943940331A5024034797 @default.
- W2943940331 hasAuthorship W2943940331A5049624036 @default.
- W2943940331 hasAuthorship W2943940331A5051093827 @default.
- W2943940331 hasAuthorship W2943940331A5053644078 @default.
- W2943940331 hasAuthorship W2943940331A5062349053 @default.
- W2943940331 hasAuthorship W2943940331A5066953827 @default.
- W2943940331 hasAuthorship W2943940331A5070156610 @default.
- W2943940331 hasAuthorship W2943940331A5076318490 @default.
- W2943940331 hasBestOaLocation W29439403311 @default.
- W2943940331 hasConcept C118487528 @default.
- W2943940331 hasConcept C126322002 @default.
- W2943940331 hasConcept C134018914 @default.
- W2943940331 hasConcept C142724271 @default.
- W2943940331 hasConcept C185592680 @default.
- W2943940331 hasConcept C204232928 @default.
- W2943940331 hasConcept C25498285 @default.
- W2943940331 hasConcept C2776768464 @default.
- W2943940331 hasConcept C2777624874 @default.
- W2943940331 hasConcept C2778609137 @default.
- W2943940331 hasConcept C2780495277 @default.
- W2943940331 hasConcept C2780827179 @default.
- W2943940331 hasConcept C2780837183 @default.
- W2943940331 hasConcept C2780876595 @default.