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- W2943996905 abstract "Like other invertebrates, silkworms also encounter a problem from microbial infection includingfrom RNA viruses. In insects, RNA interference acts as a natural anitiviral response to RNA virusinfection. Especially in Drosophila, it is proved that dicer mediated RNA interference directs innateimmunity against RNA viruses. This information prompted us to identify similar RNAse III (dicer) genein mulberry silkworm Bombyx mori. The Drosophila dicer gene was BLAST-searched with B. morigenome and single contig (GenBank accession n° AADK01001038) showed maximum homology withdicer gene, through which the RNAse III gene sequence was identified in the genome of silkworm B.mori. The RNAse III domain was present in the three regions with the length of 278 bp, 277 bp and185 bp in the contig, possibly these three regions form exons. The primers were designed for three B.mori RNAse III regions and amplified through PCR. The region I was amplified only in pure Mysoresilkworm strain whereas all three regions were amplified in Daizo strain. The PCR product sequenceswere translated and showed RNAse III domain with in the amplified product. The predicted B. moriRNAse III domain had phylogenetic relationship with other insect dicer genes. We presume that thisRNAse III (dicer) would protect B. mori larvae from invading RNA viruses, which exists in the otherinsects." @default.
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- W2943996905 date "2007-02-26" @default.
- W2943996905 modified "2023-09-26" @default.
- W2943996905 title "Identification of a putative RNAse III (dicer homolog) gene in silkworm Bombyx mori" @default.
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