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- W29439997 abstract "The massculture of marine phytoflagellates such as Protogonyaulax catenella, Prorocentrum minimum and Chattonella antiqua in 10 l or 100 l glass tanks have been examined. Filtered sea water or commercial sea salt solution, fortified with nutrients, vitamins and EDTA-Fe and -Mn was used as the culture medium after filter sterilization. Culture temperatures are 20–23°C and light intensity supplied by white fluorescent lamps was about 5000 lux under conditions of 14 hrs. light and 10 hrs. dark. The 100 l tank was equipped with a pH controller supplying CO 2 gas to keep the pH of the medium from 8.2 to 8.7. In the latter period of the exponential phase of the cell growth, half or three quarters of the culture was harvested and fresh medium was supplied aseptically. The cell yield of P.catenella was 33.2 g(dry basis) in 39 days with 400 l . The total PSP toxicities were 329,000 MU; of P.minimum , 32 g in 33 days with 410 l media; of C.antiqua , 1.2×10 10 cells corresponding to 194 g on wet basis in 42 days with 440 l . These materials are now being used in chemical analyses." @default.
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- W29439997 date "1983-01-01" @default.
- W29439997 modified "2023-09-27" @default.
- W29439997 title "MASSCULTURE OF MARINE PHYTOFLAGELLATES: AN APPROACH TO NEW SOURCES OF BIOLOGICALLY ACTIVE COMPOUNDS" @default.
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- W29439997 doi "https://doi.org/10.1016/b978-0-08-029223-6.50027-6" @default.
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