FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2944200311> ?p ?o ?g. }

• W2944200311 abstract "SUMMARY Methylation of histone 3 at lysine 9 (H3K9) is widely regarded as a major roadblock for cellular reprogramming and interference with associated methyltransferases such as EHMT1 and EHMT2 (also known as GLP and G9A, respectively) increases the efficiencies at which induced pluripotent stem cells (iPSCs) can be derived. Activation of histone and DNA demethylases by ascorbic acid (AA) has become a common approach to facilitate the extensive epigenetic remodeling required for iPSC formation, but possible functional interactions between the H3K9 methylation machinery and AA-stimulated enzymes remain insufficiently explored. Here we show that reduction of EHMT1/2 activity counteracts iPSC formation in an optimized reprogramming system in the presence of AA. Mechanistically, EHMT1/2 activity under these conditions is required for efficient downregulation of somatic genes and transition into an epithelial state. Of note, transient inhibition of EHMT1/2 during reprogramming yields iPSCs that fail to efficiently give rise to viable mice, suggesting persistent molecular defects in these cells. Genetic interference with the H3K9 demethylase KDM3B ameliorated the adverse effect of EHMT1/2 inhibition on iPSC formation. Together, our observations document novel functions of H3K9 methyltransferases during iPSC formation and suggest that the balancing of AA-stimulated enzymes by EHMT1/2 supports efficient and error-free iPSC reprogramming to pluripotency." @default.
• W2944200311 created "2019-05-16" @default.
• W2944200311 creator A5000125068 @default.
• W2944200311 creator A5009377586 @default.
• W2944200311 creator A5017862120 @default.
• W2944200311 creator A5017913027 @default.
• W2944200311 creator A5020692623 @default.
• W2944200311 creator A5032150067 @default.
• W2944200311 creator A5035865925 @default.
• W2944200311 creator A5046792632 @default.
• W2944200311 creator A5051845185 @default.
• W2944200311 creator A5067756034 @default.
• W2944200311 creator A5073613510 @default.
• W2944200311 creator A5076510486 @default.
• W2944200311 creator A5089245094 @default.
• W2944200311 creator A5089743440 @default.
• W2944200311 creator A5091863911 @default.
• W2944200311 date "2019-05-10" @default.
• W2944200311 modified "2023-09-26" @default.
• W2944200311 title "Context-dependent requirement of H3K9 methyltransferase activity during cellular reprogramming to iPSCs" @default.
• W2944200311 cites W1968190628 @default.
• W2944200311 cites W1974012180 @default.
• W2944200311 cites W1979513200 @default.
• W2944200311 cites W1980909928 @default.
• W2944200311 cites W2005063364 @default.
• W2944200311 cites W2010672807 @default.
• W2944200311 cites W2018051857 @default.
• W2944200311 cites W2018113993 @default.
• W2944200311 cites W2020423861 @default.
• W2944200311 cites W2029387074 @default.
• W2944200311 cites W2032668536 @default.
• W2944200311 cites W2038815755 @default.
• W2944200311 cites W2038963685 @default.
• W2944200311 cites W2045619208 @default.
• W2944200311 cites W2046743171 @default.
• W2944200311 cites W2063296278 @default.
• W2944200311 cites W2064297857 @default.
• W2944200311 cites W2073716029 @default.
• W2944200311 cites W2073979766 @default.
• W2944200311 cites W2076047032 @default.
• W2944200311 cites W2084562248 @default.
• W2944200311 cites W2084885369 @default.
• W2944200311 cites W2095626300 @default.
• W2944200311 cites W2105893097 @default.
• W2944200311 cites W2108234281 @default.
• W2944200311 cites W2117009494 @default.
• W2944200311 cites W2124207075 @default.
• W2944200311 cites W2134526812 @default.
• W2944200311 cites W2140729960 @default.
• W2944200311 cites W2141856502 @default.
• W2944200311 cites W2142744987 @default.
• W2944200311 cites W2147284744 @default.
• W2944200311 cites W2151522898 @default.
• W2944200311 cites W2152239989 @default.
• W2944200311 cites W2163771097 @default.
• W2944200311 cites W2167110353 @default.
• W2944200311 cites W2192789229 @default.
• W2944200311 cites W2290873962 @default.
• W2944200311 cites W2338742132 @default.
• W2944200311 cites W2593037083 @default.
• W2944200311 cites W2594452811 @default.
• W2944200311 cites W2608155867 @default.
• W2944200311 cites W2735414420 @default.
• W2944200311 cites W2741617449 @default.
• W2944200311 cites W2772941916 @default.
• W2944200311 cites W2775166823 @default.
• W2944200311 cites W2784807975 @default.
• W2944200311 cites W2801924666 @default.
• W2944200311 cites W2884502503 @default.
• W2944200311 cites W2891055002 @default.
• W2944200311 cites W2896953490 @default.
• W2944200311 cites W2921676625 @default.
• W2944200311 cites W786500739 @default.
• W2944200311 doi "https://doi.org/10.1101/634949" @default.
• W2944200311 hasPublicationYear "2019" @default.
• W2944200311 type Work @default.
• W2944200311 sameAs 2944200311 @default.
• W2944200311 citedByCount "0" @default.
• W2944200311 crossrefType "posted-content" @default.
• W2944200311 hasAuthorship W2944200311A5000125068 @default.
• W2944200311 hasAuthorship W2944200311A5009377586 @default.
• W2944200311 hasAuthorship W2944200311A5017862120 @default.
• W2944200311 hasAuthorship W2944200311A5017913027 @default.
• W2944200311 hasAuthorship W2944200311A5020692623 @default.
• W2944200311 hasAuthorship W2944200311A5032150067 @default.
• W2944200311 hasAuthorship W2944200311A5035865925 @default.
• W2944200311 hasAuthorship W2944200311A5046792632 @default.
• W2944200311 hasAuthorship W2944200311A5051845185 @default.
• W2944200311 hasAuthorship W2944200311A5067756034 @default.
• W2944200311 hasAuthorship W2944200311A5073613510 @default.
• W2944200311 hasAuthorship W2944200311A5076510486 @default.
• W2944200311 hasAuthorship W2944200311A5089245094 @default.
• W2944200311 hasAuthorship W2944200311A5089743440 @default.
• W2944200311 hasAuthorship W2944200311A5091863911 @default.
• W2944200311 hasBestOaLocation W29442003111 @default.
• W2944200311 hasConcept C104317684 @default.
• W2944200311 hasConcept C107459253 @default.
• W2944200311 hasConcept C145103041 @default.
• W2944200311 hasConcept C1491633281 @default.
• W2944200311 hasConcept C150194340 @default.

• next