Matches in SemOpenAlex for { <https://semopenalex.org/work/W2944334002> ?p ?o ?g. }
- W2944334002 endingPage "168" @default.
- W2944334002 startingPage "163" @default.
- W2944334002 abstract "We assessed effect of Akkermansia muciniphila and its extracellular vesicles on toll-like receptors and tight junction expression in human epithelial colorectal adenocarcinoma cells (Caco-2).The intestinal microbiota plays an important role in the intestinal homeostasis through its metabolites and derivatives. Interacting with immune cells and intestinal epithelial pattern recognition receptors (PRRs), intestinal microbiota regulates the function of the digestive barrier and inflammation caused by the metabolic diseases.A. muciniphila was cultured on a mucin-containing medium and its EVs was extracted by ultracentrifugation. This bacterium was treated in the MOI=10 and its EVs at the concentrations of 0.1, 0.5 and 5 µg on Caco-2 cells. After 24 hours, the expression of tight junction and toll-like receptor genes were investigated by quantitative real time PCR method.A. muciniphila increased the expression of tlr2 and tlr4. However, EVs at all of the concentrations showed a decrease in tlr4 expression. EVs at the concentrations of 0.1 and 0.5 µg/ml decreased the expression of tlr2. A. muciniphila significantly increased the expression of ocldn and cldn4. Both this bacterium and EVs increased the expression of zo2 in the cell line. Furthermore, this data show that A. muciniphila derived EVs have a dose-independent effect on Caco-2 cells.This preliminary research shows A. muciniphila and its EVs both may increase the integrity of the intestinal barrier. A. muciniphila derived EVs also reduces the inflammation so that EVs of this bacterium can be used as an appropriate target for the treatment of metabolic syndrome and inflammatory bowel diseases." @default.
- W2944334002 created "2019-05-16" @default.
- W2944334002 creator A5005951655 @default.
- W2944334002 creator A5006829613 @default.
- W2944334002 creator A5034204741 @default.
- W2944334002 creator A5050935757 @default.
- W2944334002 creator A5061244322 @default.
- W2944334002 creator A5067184137 @default.
- W2944334002 creator A5071946331 @default.
- W2944334002 creator A5081662497 @default.
- W2944334002 creator A5084050163 @default.
- W2944334002 creator A5088818808 @default.
- W2944334002 date "2019-01-01" @default.
- W2944334002 modified "2023-10-02" @default.
- W2944334002 title "Comparative study of effect of Akkermansia muciniphila and its extracellular vesicles on toll-like receptors and tight junction." @default.
- W2944334002 cites W1967006152 @default.
- W2944334002 cites W1974819402 @default.
- W2944334002 cites W1999471314 @default.
- W2944334002 cites W2032698686 @default.
- W2944334002 cites W2051349444 @default.
- W2944334002 cites W2052554412 @default.
- W2944334002 cites W2077248729 @default.
- W2944334002 cites W2086465908 @default.
- W2944334002 cites W2102941698 @default.
- W2944334002 cites W2133010654 @default.
- W2944334002 cites W2134241692 @default.
- W2944334002 cites W2139334262 @default.
- W2944334002 cites W2147790901 @default.
- W2944334002 cites W2149362751 @default.
- W2944334002 cites W2155924614 @default.
- W2944334002 cites W2160583419 @default.
- W2944334002 cites W2267861477 @default.
- W2944334002 cites W2288329334 @default.
- W2944334002 cites W2559267012 @default.
- W2944334002 cites W2567684007 @default.
- W2944334002 cites W2568502991 @default.
- W2944334002 cites W2594894350 @default.
- W2944334002 cites W2616943065 @default.
- W2944334002 cites W2746341193 @default.
- W2944334002 cites W2765564884 @default.
- W2944334002 cites W2776943472 @default.
- W2944334002 cites W2787932709 @default.
- W2944334002 cites W321706625 @default.
- W2944334002 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6536015" @default.
- W2944334002 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31191842" @default.
- W2944334002 hasPublicationYear "2019" @default.
- W2944334002 type Work @default.
- W2944334002 sameAs 2944334002 @default.
- W2944334002 citedByCount "30" @default.
- W2944334002 countsByYear W29443340022019 @default.
- W2944334002 countsByYear W29443340022020 @default.
- W2944334002 countsByYear W29443340022021 @default.
- W2944334002 countsByYear W29443340022022 @default.
- W2944334002 countsByYear W29443340022023 @default.
- W2944334002 crossrefType "journal-article" @default.
- W2944334002 hasAuthorship W2944334002A5005951655 @default.
- W2944334002 hasAuthorship W2944334002A5006829613 @default.
- W2944334002 hasAuthorship W2944334002A5034204741 @default.
- W2944334002 hasAuthorship W2944334002A5050935757 @default.
- W2944334002 hasAuthorship W2944334002A5061244322 @default.
- W2944334002 hasAuthorship W2944334002A5067184137 @default.
- W2944334002 hasAuthorship W2944334002A5071946331 @default.
- W2944334002 hasAuthorship W2944334002A5081662497 @default.
- W2944334002 hasAuthorship W2944334002A5084050163 @default.
- W2944334002 hasAuthorship W2944334002A5088818808 @default.
- W2944334002 hasConcept C136449434 @default.
- W2944334002 hasConcept C1491633281 @default.
- W2944334002 hasConcept C170493617 @default.
- W2944334002 hasConcept C177779419 @default.
- W2944334002 hasConcept C179264091 @default.
- W2944334002 hasConcept C193176779 @default.
- W2944334002 hasConcept C203014093 @default.
- W2944334002 hasConcept C2775941767 @default.
- W2944334002 hasConcept C2776070231 @default.
- W2944334002 hasConcept C2776709828 @default.
- W2944334002 hasConcept C2776914184 @default.
- W2944334002 hasConcept C2777907754 @default.
- W2944334002 hasConcept C2779676784 @default.
- W2944334002 hasConcept C2781236682 @default.
- W2944334002 hasConcept C42062724 @default.
- W2944334002 hasConcept C51911345 @default.
- W2944334002 hasConcept C523546767 @default.
- W2944334002 hasConcept C539455810 @default.
- W2944334002 hasConcept C54355233 @default.
- W2944334002 hasConcept C55493867 @default.
- W2944334002 hasConcept C86803240 @default.
- W2944334002 hasConcept C8891405 @default.
- W2944334002 hasConcept C89423630 @default.
- W2944334002 hasConcept C95444343 @default.
- W2944334002 hasConceptScore W2944334002C136449434 @default.
- W2944334002 hasConceptScore W2944334002C1491633281 @default.
- W2944334002 hasConceptScore W2944334002C170493617 @default.
- W2944334002 hasConceptScore W2944334002C177779419 @default.
- W2944334002 hasConceptScore W2944334002C179264091 @default.
- W2944334002 hasConceptScore W2944334002C193176779 @default.
- W2944334002 hasConceptScore W2944334002C203014093 @default.
- W2944334002 hasConceptScore W2944334002C2775941767 @default.
- W2944334002 hasConceptScore W2944334002C2776070231 @default.