FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2944601004> ?p ?o ?g. }

• W2944601004 endingPage "43" @default.
• W2944601004 startingPage "33" @default.
• W2944601004 abstract "Plants and cyanobacteria are promising heterologous hosts for metabolic engineering, and particularly suited for expression of cytochrome P450 (P450s), enzymes that catalyse key steps in biosynthetic pathways leading to valuable natural products such as alkaloids, terpenoids and phenylpropanoids. P450s are often difficult to express and require a membrane-bound NADPH-dependent reductase, complicating their use in metabolic engineering and bio-production. We previously demonstrated targeting of heterologous P450s to thylakoid membranes both in N. benthamiana chloroplasts and cyanobacteria, and functional substitution of their native reductases with the photosynthetic apparatus via the endogenous soluble electron carrier ferredoxin. However, because ferredoxin acts as a sorting hub for photosynthetic reducing power, there is fierce competition for reducing equivalents, which limits photosynthesis-driven P450 output. This study compares the ability of four electron carriers to increase photosynthesis-driven P450 activity. These carriers, three plant ferredoxins and a flavodoxin-like engineered protein derived from cytochrome P450 reductase, show only modest differences in their electron transfer to our model P450, CYP79A1 in vitro. However, only the flavodoxin-like carrier supplies appreciable reducing power in the presence of competition for reduced ferredoxin, because it possesses a redox potential that renders delivery of reducing equivalents to endogenous processes inefficient. We further investigate the efficacy of these electron carrier proteins in vivo by expressing them transiently in N. benthamiana fused to CYP79A1. All but one of the fusion enzymes show improved sequestration of photosynthetic reducing power. Fusion with the flavodoxin-like carrier offers the greatest improvement in this comparison - nearly 25-fold on a per protein basis. Thus, this study demonstrates that synthetic electron transfer pathways with optimal redox potentials can alleviate the problem of endogenous competition for reduced ferredoxin and sets out a new metabolic engineering strategy useful for producing valuable natural products." @default.
• W2944601004 created "2019-05-16" @default.
• W2944601004 creator A5023717784 @default.
• W2944601004 creator A5030456314 @default.
• W2944601004 creator A5031522410 @default.
• W2944601004 creator A5040328094 @default.
• W2944601004 creator A5043498347 @default.
• W2944601004 creator A5049748974 @default.
• W2944601004 creator A5068786051 @default.
• W2944601004 creator A5073096871 @default.
• W2944601004 creator A5083444369 @default.
• W2944601004 creator A5086609761 @default.
• W2944601004 date "2019-09-01" @default.
• W2944601004 modified "2023-10-14" @default.
• W2944601004 title "Defining optimal electron transfer partners for light-driven cytochrome P450 reactions" @default.
• W2944601004 cites W116253160 @default.
• W2944601004 cites W1519266993 @default.
• W2944601004 cites W1604244147 @default.
• W2944601004 cites W1760074260 @default.
• W2944601004 cites W1903986304 @default.
• W2944601004 cites W1928349960 @default.
• W2944601004 cites W1964469970 @default.
• W2944601004 cites W1966610162 @default.
• W2944601004 cites W1971439989 @default.
• W2944601004 cites W1986080936 @default.
• W2944601004 cites W1986506616 @default.
• W2944601004 cites W1989037685 @default.
• W2944601004 cites W2003117374 @default.
• W2944601004 cites W2003672482 @default.
• W2944601004 cites W2008216530 @default.
• W2944601004 cites W2010244877 @default.
• W2944601004 cites W2019209576 @default.
• W2944601004 cites W2020601582 @default.
• W2944601004 cites W2024926027 @default.
• W2944601004 cites W2029903300 @default.
• W2944601004 cites W2035873965 @default.
• W2944601004 cites W2049213830 @default.
• W2944601004 cites W2057396845 @default.
• W2944601004 cites W2057911693 @default.
• W2944601004 cites W2065784807 @default.
• W2944601004 cites W2069697511 @default.
• W2944601004 cites W2069820597 @default.
• W2944601004 cites W2070006281 @default.
• W2944601004 cites W2075727429 @default.
• W2944601004 cites W2078880422 @default.
• W2944601004 cites W2078944494 @default.
• W2944601004 cites W2088099835 @default.
• W2944601004 cites W2094978678 @default.
• W2944601004 cites W2111492898 @default.
• W2944601004 cites W2120772351 @default.
• W2944601004 cites W2125314148 @default.
• W2944601004 cites W2132926880 @default.
• W2944601004 cites W2134069782 @default.
• W2944601004 cites W2140615337 @default.
• W2944601004 cites W2140729156 @default.
• W2944601004 cites W2141060260 @default.
• W2944601004 cites W2154893969 @default.
• W2944601004 cites W2155220427 @default.
• W2944601004 cites W2157445553 @default.
• W2944601004 cites W2157727831 @default.
• W2944601004 cites W2159347934 @default.
• W2944601004 cites W2168640586 @default.
• W2944601004 cites W2194706069 @default.
• W2944601004 cites W2295491699 @default.
• W2944601004 cites W2311276428 @default.
• W2944601004 cites W2311798020 @default.
• W2944601004 cites W2317456426 @default.
• W2944601004 cites W2328286101 @default.
• W2944601004 cites W2334381352 @default.
• W2944601004 cites W2344094488 @default.
• W2944601004 cites W2407275875 @default.
• W2944601004 cites W2444500095 @default.
• W2944601004 cites W2501269559 @default.
• W2944601004 cites W2512653639 @default.
• W2944601004 cites W2528861774 @default.
• W2944601004 cites W2556049317 @default.
• W2944601004 cites W2585154652 @default.
• W2944601004 cites W2591665786 @default.
• W2944601004 cites W2731784911 @default.
• W2944601004 cites W2736427469 @default.
• W2944601004 cites W2795292980 @default.
• W2944601004 cites W2796818985 @default.
• W2944601004 cites W2801407875 @default.
• W2944601004 cites W2803747034 @default.
• W2944601004 cites W2809522305 @default.
• W2944601004 cites W2884544665 @default.
• W2944601004 cites W2903414239 @default.
• W2944601004 cites W2934202541 @default.
• W2944601004 cites W2947428625 @default.
• W2944601004 cites W345019790 @default.
• W2944601004 cites W91551770 @default.
• W2944601004 doi "https://doi.org/10.1016/j.ymben.2019.05.003" @default.
• W2944601004 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31091467" @default.
• W2944601004 hasPublicationYear "2019" @default.
• W2944601004 type Work @default.
• W2944601004 sameAs 2944601004 @default.
• W2944601004 citedByCount "22" @default.
• W2944601004 countsByYear W29446010042020 @default.

• next