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- W2944671330 abstract "Abstract Bead-based activation is widely-used for ex vivo expansion of T cells for either research or clinical purposes. Despite its wide use, culture conditions that can potentially affect the efficiency of bead-based T cell activation has not been extensively documented. With the help of computationally-driven experimental investigations of basic culturing factors, we found that culture density, bead-to-cell ratio, and debeading time can have a major impact on the efficiency of bead-based T cell activation for short-term cultures. Furthermore, discrepancies across expected and observed activation efficiencies helped discover interesting artifacts of bead-based T cell activation. Human primary T cells were imaged together with activation beads at 20X magnification after three hours of culturing at varying confluencies and bead-to-cell ratios." @default.
- W2944671330 created "2019-05-16" @default.
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- W2944671330 date "2019-05-07" @default.
- W2944671330 modified "2023-09-25" @default.
- W2944671330 title "Computational and experimental optimization of T cell activation" @default.
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- W2944671330 doi "https://doi.org/10.1101/629857" @default.
- W2944671330 hasPublicationYear "2019" @default.
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