FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2944829321> ?p ?o ?g. }

• W2944829321 endingPage "1401" @default.
• W2944829321 startingPage "1390" @default.
• W2944829321 abstract "Objective— Activation of coagulation FXI (factor XI) by FXIIa (activated factor XII) is a prothrombotic process. The endothelium is known to play an antithrombotic role by limiting thrombin generation and platelet activation. It is unknown whether the antithrombotic role of the endothelium includes sequestration of FXIa (activated factor XI) activity. This study aims to determine the role of endothelial cells (ECs) in the regulation of the intrinsic pathway of coagulation. Approach and Results— Using a chromogenic assay, we observed that human umbilical veins ECs selectively blocked FXIa yet supported kallikrein and FXIIa activity. Western blotting and mass spectrometry analyses revealed that FXIa formed a complex with endothelial PAI-1 (plasminogen activator inhibitor-1). Blocking endothelial PAI-1 increased the cleavage of a chromogenic substrate by FXIa and the capacity of FXIa to promote fibrin formation in plasma. Western blot and immunofluorescence analyses showed that FXIa–PAI-1 complexes were either released into the media or trafficked to the early and late endosomes and lysosomes of ECs. When baboons were challenged with Staphylococcus aureus to induce a prothrombotic phenotype, an increase in circulating FXIa–PAI-1 complex levels was detected by ELISA within 2 to 8 hours postchallenge. Conclusions— PAI-1 forms a complex with FXIa on ECs, blocking its activity and inducing the clearance and degradation of FXIa. Circulating FXIa–PAI-1 complexes were detected in a baboon model of S. aureus sepsis. Although ECs support kallikrein and FXIIa activity, inhibition of FXIa by ECs may promote the clearance of intravascular FXIa. Visual Overview— An online visual overview is available for this article." @default.
• W2944829321 created "2019-05-29" @default.
• W2944829321 creator A5019186471 @default.
• W2944829321 creator A5021496734 @default.
• W2944829321 creator A5023330760 @default.
• W2944829321 creator A5036237322 @default.
• W2944829321 creator A5050978437 @default.
• W2944829321 creator A5056174184 @default.
• W2944829321 creator A5071210838 @default.
• W2944829321 creator A5074003801 @default.
• W2944829321 creator A5077036119 @default.
• W2944829321 date "2019-07-01" @default.
• W2944829321 modified "2023-09-30" @default.
• W2944829321 title "Endothelial PAI-1 (Plasminogen Activator Inhibitor-1) Blocks the Intrinsic Pathway of Coagulation, Inducing the Clearance and Degradation of FXIa (Activated Factor XI)" @default.
• W2944829321 cites W143880323 @default.
• W2944829321 cites W1487546257 @default.
• W2944829321 cites W1522151606 @default.
• W2944829321 cites W1633360739 @default.
• W2944829321 cites W1854845471 @default.
• W2944829321 cites W1860833529 @default.
• W2944829321 cites W1971144371 @default.
• W2944829321 cites W1974208990 @default.
• W2944829321 cites W1984954505 @default.
• W2944829321 cites W1995621634 @default.
• W2944829321 cites W2001124978 @default.
• W2944829321 cites W2002431523 @default.
• W2944829321 cites W2003422570 @default.
• W2944829321 cites W2006571134 @default.
• W2944829321 cites W2013031367 @default.
• W2944829321 cites W2016111772 @default.
• W2944829321 cites W2019191000 @default.
• W2944829321 cites W2027322928 @default.
• W2944829321 cites W2042466479 @default.
• W2944829321 cites W2047425163 @default.
• W2944829321 cites W2053264658 @default.
• W2944829321 cites W2056261557 @default.
• W2944829321 cites W2061465029 @default.
• W2944829321 cites W2063706685 @default.
• W2944829321 cites W2074704061 @default.
• W2944829321 cites W2075742540 @default.
• W2944829321 cites W2075761721 @default.
• W2944829321 cites W2079416873 @default.
• W2944829321 cites W2084336361 @default.
• W2944829321 cites W2086758849 @default.
• W2944829321 cites W2100451345 @default.
• W2944829321 cites W2113525532 @default.
• W2944829321 cites W2123844702 @default.
• W2944829321 cites W2125249931 @default.
• W2944829321 cites W2166268582 @default.
• W2944829321 cites W2172191543 @default.
• W2944829321 cites W217628581 @default.
• W2944829321 cites W2201309601 @default.
• W2944829321 cites W2284471329 @default.
• W2944829321 cites W2313919223 @default.
• W2944829321 cites W2333642109 @default.
• W2944829321 cites W2338854794 @default.
• W2944829321 cites W2394807357 @default.
• W2944829321 cites W2401721742 @default.
• W2944829321 cites W2409296984 @default.
• W2944829321 cites W2533833743 @default.
• W2944829321 cites W2776355388 @default.
• W2944829321 cites W2808938206 @default.
• W2944829321 cites W2917214053 @default.
• W2944829321 cites W32427409 @default.
• W2944829321 cites W4237546580 @default.
• W2944829321 cites W64750453 @default.
• W2944829321 cites W974578078 @default.
• W2944829321 doi "https://doi.org/10.1161/atvbaha.119.312619" @default.
• W2944829321 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6597189" @default.
• W2944829321 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31242030" @default.
• W2944829321 hasPublicationYear "2019" @default.
• W2944829321 type Work @default.
• W2944829321 sameAs 2944829321 @default.
• W2944829321 citedByCount "19" @default.
• W2944829321 countsByYear W29448293212019 @default.
• W2944829321 countsByYear W29448293212020 @default.
• W2944829321 countsByYear W29448293212021 @default.
• W2944829321 countsByYear W29448293212022 @default.
• W2944829321 countsByYear W29448293212023 @default.
• W2944829321 crossrefType "journal-article" @default.
• W2944829321 hasAuthorship W2944829321A5019186471 @default.
• W2944829321 hasAuthorship W2944829321A5021496734 @default.
• W2944829321 hasAuthorship W2944829321A5023330760 @default.
• W2944829321 hasAuthorship W2944829321A5036237322 @default.
• W2944829321 hasAuthorship W2944829321A5050978437 @default.
• W2944829321 hasAuthorship W2944829321A5056174184 @default.
• W2944829321 hasAuthorship W2944829321A5071210838 @default.
• W2944829321 hasAuthorship W2944829321A5074003801 @default.
• W2944829321 hasAuthorship W2944829321A5077036119 @default.
• W2944829321 hasBestOaLocation W29448293211 @default.
• W2944829321 hasConcept C104317684 @default.
• W2944829321 hasConcept C126322002 @default.
• W2944829321 hasConcept C134018914 @default.
• W2944829321 hasConcept C153911025 @default.
• W2944829321 hasConcept C185592680 @default.
• W2944829321 hasConcept C202751555 @default.
• W2944829321 hasConcept C203014093 @default.
• W2944829321 hasConcept C2776415932 @default.

• next