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- W2944964482 abstract "Abstract We developed a green infrared‐assisted extraction method for the determination of 3′‐hydroxy puerarin, puerarin, daidzin, genistin, and daidzein in Pueraria lobata . After a simultaneous optimization of Box–Behnken design and desirability function, the developed method provided better isoflavone extraction efficiencies with a lower solvent consumption and shorter extraction time than other methods. The optimized extraction used an extraction time of 8 min, solid–liquid ratio of 1:100, and ethanol volume fraction of 60%. Additionally, ultra‐high performance liquid chromatography was used for identification and quantification of the five isoflavones. Our method showed good linearity ( r >0.999) over wide concentration ranges. The ranges for the limits of detection and quantitation were 0.03–0.09 μg/mL and 0.14–0.44 μg/mL, respectively, the recovery range was 98.38–103.37%, and the run time was 5.5 min. This approach is efficient, economical, and eco‐friendly, and shows promise for rapid extraction and quantitative analysis of bioactive isoflavones from Pueraria lobata ." @default.
- W2944964482 created "2019-05-29" @default.
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- W2944964482 date "2019-05-22" @default.
- W2944964482 modified "2023-09-27" @default.
- W2944964482 title "Green, infrared‐assisted extraction based on statistical modeling for ultra‐high performance liquid chromatography determination of bioactive isoflavones from <i>Puerariae Lobatae</i>" @default.
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- W2944964482 doi "https://doi.org/10.1002/sscp.201900030" @default.
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