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- W2945077237 abstract "Abstract To date, several cytoplasms of wild species have been introduced to Brassica juncea , for inducing cytoplasmic male sterility (CMS). One of the causal genes of CMS is orf108 , which is widely distributed in Brassicaceae including Brassica oxyrrhina . To further understand the origin of orf108 , we assembled the complete mitochondrial genome sequence of B. oxyrrhina . We also determined the DNA sequences upstream of atp1 including orf108 for D. erucoides and five Sinapis species. The orf108 was definitively placed in the mitochondrial genome of B. oxyrrhina consisting of 247,936 bp. In S. alba , previously reported to possess orf108 , the gene was not present, whereas Sinapis arvensis and Sinapis turgida contained orf108 . The orf108 sequence in the two Sinapis species showed a novel nucleotide substitution compared to D. erucoide s. Northern hybridization suggested, furthermore, that orf108 mRNA was processed in the two species similarly to that in B. oxyrrhina . The results clarified the interspecific differentiation of orf108 ‐ apt1 region in Sinapis ." @default.
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- W2945077237 date "2019-05-21" @default.
- W2945077237 modified "2023-10-16" @default.
- W2945077237 title "Complete mitochondrial genome sequence of Brassica oxyrrhina and comparative analysis of the region containing orf108 , a male sterility gene for mustard ( Brassica juncea ), among B. oxyrrhina , Diplotaxis erucoides and Sinapis species" @default.
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- W2945077237 doi "https://doi.org/10.1111/pbr.12710" @default.
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