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• W2945109062 startingPage "175" @default.
• W2945109062 abstract "Mitochondrial dysfunction is linked to many neurological diseases; therefore, the ability to measure mitochondrial function is of great use for researching disease and testing potential therapeutics. Here we describe a high-content assay to simultaneously measure mitochondrial membrane potential, morphology and cell viability in iPSC-derived neurons. Neurons are seeded into plates suitable for fluorescent microscopy, stained with the mitochondrial membrane potential-dependent dye TMRM, cytoplasmic dye Calcein AM, and nuclear stain Hoechst 33342. Images are acquired in live cells and analyzed using automated image analysis software." @default.
• W2945109062 created "2019-05-29" @default.
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• W2945109062 date "2019-01-01" @default.
• W2945109062 modified "2023-10-01" @default.
• W2945109062 title "High-Content Analysis of Mitochondrial Function in iPSC-Derived Neurons" @default.
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• W2945109062 doi "https://doi.org/10.1007/978-1-4939-9477-9_16" @default.
• W2945109062 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31124115" @default.
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