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- W2945121186 abstract "Many chemical reactions in the cell are thermodynamically unfavorable. To overcome this barrier, the energy released from the hydrolysis of adenosine triphosphate (ATP) is coupled to these reactions via ATP hydrolyzing enzymes known as ATPases. These enzymes are ubiquitous in nature and frequently act as molecular motors in processes ranging from DNA replication to protein degradation. Assays that characterize ATPase activity in vitro are important tools to gain insight into their functions in vivo. Here, we describe a direct and flexible thin-layer chromatography method for detecting ATPase activity using radiolabeled ATP. Additionally, we describe a high-throughput coupled reaction assay pairing ATP hydrolysis with nicotinamide adenine dinucleotide (NADH) oxidation to monitor ATP hydrolysis in real time." @default.
- W2945121186 created "2019-05-29" @default.
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- W2945121186 date "2019-01-01" @default.
- W2945121186 modified "2023-09-26" @default.
- W2945121186 title "Thin-Layer Chromatography and Real-Time Coupled Assays to Measure ATP Hydrolysis" @default.
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- W2945121186 doi "https://doi.org/10.1007/978-1-4939-9500-4_15" @default.
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