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- W2945179924 abstract "The vaccine candidate comprising the genes that code for the B subunit of the heat labile enterotoxin(LT B) and the heat stable enterotoxin(ST) of enterotoxigenic Escherichia coli (ETEC) had been constructed by recombinant genetic techniques .The 5' terminus of the gene encoding pro-ST was genetically fused to the 3' terminus of the LT -13 gene.The pro-ST gene contains mature ST sequence and pro sequence which codes for the pro region of ST precursors，and a mplifie dby PCRfrom pSLM004.To reduce toxicity of the ST in vitro was substituted Leu for Ala residue at position 14 of ST by oligonucleotide-directed site mutagenesis .For this construction，the expres-sion of ST antigenicity and LT antigenicity were obtained when a five-amino-acid or a nine-aminoacid linker were included between the LT -13 and pro-ST moieties.The LT B/ pro- S T fusion peptides possessed no enterotoxic activity of Escherichia coli heat stable and head-labile enterotoxins ,and re-tained the ability to bind GMl ganglioside .More importantly，these LT-B/ pro- S T fusion peptides were immunogenic .The preparations containing the hybrid molecule elicited special antibodies that were able to recognize native toxin in vitro." @default.
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- W2945179924 date "1999-01-01" @default.
- W2945179924 modified "2023-09-25" @default.
- W2945179924 title "Gene Flsion and Expression of Heat labile and Heat stable of Enterotoxigenic Escherichia coli" @default.
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