FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2945283715> ?p ?o ?g. }

• W2945283715 endingPage "8" @default.
• W2945283715 startingPage "1" @default.
• W2945283715 abstract "Objective . To evaluate the role of caveolin-1 (Cav-1) in HIV-1 Tat-induced dysfunction of tight junction and amyloid β -peptide- (A β -) transferring proteins. Methods . A Cav-1 shRNA interference target sequence was cloned into the lentiviral vector pHBLV-U6-Scramble-ZsGreen-Puro and verified by double enzyme digestion and DNA sequencing. Human cerebral microvascular endothelium (HBEC-5i) cells were transduced with viral particles made in 293T cells by transfection with lentiviral packaging plasmids. HBEC-5i cells transduced with Cav-1 shRNA or Ctr shRNA were exposed to HIV-1 Tat for 24 h, and the protein and mRNA levels of the tight junction protein occludin, A β -transferring protein, receptor for advanced glycation end products (RAGE), low-density lipoprotein receptor-related protein- (LRP-) 1, and RhoA were evaluated with Western blot and real-time reverse transcription polymerase chain reaction (qRT-PCR) assays, respectively. Results . After sequencing, an RNA interference recombinant lentivirus expressing a vector targeting Cav-1 was successfully established. The recombined lentiviral particles were made by using 293T cells to package the recombined lentiviral vector. A stable monoclonal cell line with strong GFP expression was acquired with a Cav-1 knockdown rate of 85.7%. The occludin protein and mRNA levels in the Ctr shRNA group were decreased with HIV-1 Tat exposure but were upregulated in the Cav-1 shRNA group. The HIV-1 Tat-induced alterations of RAGE and LRP-1 protein and mRNA levels in the Ctr shRNA group were attenuated in the Cav-1 shRNA group. The RhoA protein levels in the Ctr shRNA group were upregulated by HIV-1 Tat exposure but were downregulated in the Cav-1 shRNA group. Conclusion . These results show that HIV-1 Tat-induced downregulation of occludin and LRP-1 and upregulation of RAGE and RhoA may result in the accumulation of A β in the brain. Silencing the Cav-1 gene with shRNA plays a key role in the protection against HIV-1 Tat-induced dysfunction of the blood-brain barrier and A β accumulation." @default.
• W2945283715 created "2019-05-29" @default.
• W2945283715 creator A5000964711 @default.
• W2945283715 creator A5005005524 @default.
• W2945283715 creator A5044120570 @default.
• W2945283715 creator A5056373734 @default.
• W2945283715 creator A5077913816 @default.
• W2945283715 date "2019-05-14" @default.
• W2945283715 modified "2023-10-14" @default.
• W2945283715 title "Role of Cav-1 in HIV-1 Tat-Induced Dysfunction of Tight Junctions and A<i>β</i>-Transferring Proteins" @default.
• W2945283715 cites W1901295637 @default.
• W2945283715 cites W1966108197 @default.
• W2945283715 cites W1986063396 @default.
• W2945283715 cites W1987400759 @default.
• W2945283715 cites W1994758284 @default.
• W2945283715 cites W2003708432 @default.
• W2945283715 cites W2019731805 @default.
• W2945283715 cites W2020025682 @default.
• W2945283715 cites W2031095496 @default.
• W2945283715 cites W2036070626 @default.
• W2945283715 cites W2043973455 @default.
• W2945283715 cites W2062091008 @default.
• W2945283715 cites W2063846665 @default.
• W2945283715 cites W2073273251 @default.
• W2945283715 cites W2076485284 @default.
• W2945283715 cites W2091277662 @default.
• W2945283715 cites W2103831817 @default.
• W2945283715 cites W2107233754 @default.
• W2945283715 cites W2115118763 @default.
• W2945283715 cites W2122694926 @default.
• W2945283715 cites W2134639090 @default.
• W2945283715 cites W2137321682 @default.
• W2945283715 cites W2158808605 @default.
• W2945283715 cites W2329336071 @default.
• W2945283715 cites W2337369609 @default.
• W2945283715 cites W2485643186 @default.
• W2945283715 cites W2610543526 @default.
• W2945283715 cites W2617653468 @default.
• W2945283715 cites W2786608785 @default.
• W2945283715 cites W2892337827 @default.
• W2945283715 doi "https://doi.org/10.1155/2019/3403206" @default.
• W2945283715 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6537002" @default.
• W2945283715 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31217837" @default.
• W2945283715 hasPublicationYear "2019" @default.
• W2945283715 type Work @default.
• W2945283715 sameAs 2945283715 @default.
• W2945283715 citedByCount "9" @default.
• W2945283715 countsByYear W29452837152019 @default.
• W2945283715 countsByYear W29452837152020 @default.
• W2945283715 countsByYear W29452837152021 @default.
• W2945283715 countsByYear W29452837152022 @default.
• W2945283715 countsByYear W29452837152023 @default.
• W2945283715 crossrefType "journal-article" @default.
• W2945283715 hasAuthorship W2945283715A5000964711 @default.
• W2945283715 hasAuthorship W2945283715A5005005524 @default.
• W2945283715 hasAuthorship W2945283715A5044120570 @default.
• W2945283715 hasAuthorship W2945283715A5056373734 @default.
• W2945283715 hasAuthorship W2945283715A5077913816 @default.
• W2945283715 hasBestOaLocation W29452837151 @default.
• W2945283715 hasConcept C104317684 @default.
• W2945283715 hasConcept C1292079 @default.
• W2945283715 hasConcept C150194340 @default.
• W2945283715 hasConcept C153911025 @default.
• W2945283715 hasConcept C166703698 @default.
• W2945283715 hasConcept C173396325 @default.
• W2945283715 hasConcept C174510640 @default.
• W2945283715 hasConcept C177779419 @default.
• W2945283715 hasConcept C185592680 @default.
• W2945283715 hasConcept C190232843 @default.
• W2945283715 hasConcept C2775962179 @default.
• W2945283715 hasConcept C2777093181 @default.
• W2945283715 hasConcept C32470452 @default.
• W2945283715 hasConcept C40767141 @default.
• W2945283715 hasConcept C54009773 @default.
• W2945283715 hasConcept C54355233 @default.
• W2945283715 hasConcept C55493867 @default.
• W2945283715 hasConcept C62478195 @default.
• W2945283715 hasConcept C67705224 @default.
• W2945283715 hasConcept C81885089 @default.
• W2945283715 hasConcept C86803240 @default.
• W2945283715 hasConcept C95444343 @default.
• W2945283715 hasConceptScore W2945283715C104317684 @default.
• W2945283715 hasConceptScore W2945283715C1292079 @default.
• W2945283715 hasConceptScore W2945283715C150194340 @default.
• W2945283715 hasConceptScore W2945283715C153911025 @default.
• W2945283715 hasConceptScore W2945283715C166703698 @default.
• W2945283715 hasConceptScore W2945283715C173396325 @default.
• W2945283715 hasConceptScore W2945283715C174510640 @default.
• W2945283715 hasConceptScore W2945283715C177779419 @default.
• W2945283715 hasConceptScore W2945283715C185592680 @default.
• W2945283715 hasConceptScore W2945283715C190232843 @default.
• W2945283715 hasConceptScore W2945283715C2775962179 @default.
• W2945283715 hasConceptScore W2945283715C2777093181 @default.
• W2945283715 hasConceptScore W2945283715C32470452 @default.
• W2945283715 hasConceptScore W2945283715C40767141 @default.
• W2945283715 hasConceptScore W2945283715C54009773 @default.
• W2945283715 hasConceptScore W2945283715C54355233 @default.
• W2945283715 hasConceptScore W2945283715C55493867 @default.

• next