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- W2945304449 endingPage "244" @default.
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- W2945304449 abstract "DNA double-strand breaks (DSBs) are a potentially lethal DNA lesions that disrupt both the physical and genetic continuity of the DNA duplex. Homologous recombination (HR) is a universally conserved genome maintenance pathway that initiates via nucleolytic processing of the broken DNA ends (resection). Eukaryotic DNA resection is catalyzed by the resectosome-a multicomponent molecular machine consisting of the nucleases DNA2 or Exonuclease 1 (EXO1), Bloom's helicase (BLM), the MRE11-RAD50-NBS1 (MRN) complex, and additional regulatory factors. Here, we describe methods for purification and single-molecule imaging and analysis of EXO1, DNA2, and BLM. We also describe how to adapt resection assays to the high-throughput single-molecule DNA curtain assay. By organizing hundreds of individual molecules on the surface of a microfluidic flowcell, DNA curtains visualize protein complexes with the required spatial and temporal resolution to resolve the molecular choreography during critical DNA-processing reactions." @default.
- W2945304449 created "2019-05-29" @default.
- W2945304449 creator A5023369714 @default.
- W2945304449 creator A5049172093 @default.
- W2945304449 creator A5080401389 @default.
- W2945304449 date "2019-01-01" @default.
- W2945304449 modified "2023-09-24" @default.
- W2945304449 title "Assembling the Human Resectosome on DNA Curtains" @default.
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- W2945304449 doi "https://doi.org/10.1007/978-1-4939-9500-4_14" @default.
- W2945304449 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6666396" @default.
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- W2945304449 hasPublicationYear "2019" @default.
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