FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2945361239> ?p ?o ?g. }

• W2945361239 endingPage "101226" @default.
• W2945361239 startingPage "101226" @default.
• W2945361239 abstract "Laminin is a major protein of the basement membrane (BM), a specialized extracellular matrix (ECM) of the artery wall. The potent oxidizing and nitrating agent peroxynitrous acid (ONOOH) is formed at sites of inflammation, and data implicate ONOOH in ECM damage and cardiovascular disease. Co-localization of 3-nitrotyrosine, a product of ONOOH-mediated tyrosine (Tyr) modification, and laminin has been reported in human atherosclerotic lesions. The sites and consequences of 3-nitrotyrosine (and related nitrated tryptophan) formation on laminin, it's self-assembly and cell interactions are poorly understood. In this study murine laminin-111 was exposed to ONOOH (1–500-fold molar excess). Nitration sites were mapped and quantified using LC-MS/MS. Mono-nitration was detected at 148 sites (126 Tyr, 22 Trp), and di-nitration at 14 sites. Label-free quantification showed enhanced nitration with increasing oxidant doses. Tyr nitration was ∼10-fold greater than at Trp. CO2 modulated damage in a site-specific manner, with most sites less extensively nitrated. 119 mono-nitration sites were identified with CO2 present, and no unique sites were detected. 23 di-nitration sites were detected, with 15 unique to the presence of CO2. Extensive modification was detected at sites involved in cell adhesion, protein-protein interactions and self-polymerization. Tyr-145 on the γ1 chain was extensively nitrated, and endothelial cells exhibited decreased adhesion to a nitrated peptide modelling this site. Modification of residues involved in self-polymerization interfered with the formation of ordered polymers as detected by scanning electron microscopy. These laminin modifications may contribute to endothelial cell dysfunction and modulate ECM structure and assembly, and thereby contribute to atherogenesis." @default.
• W2945361239 created "2019-05-29" @default.
• W2945361239 creator A5020109552 @default.
• W2945361239 creator A5034929303 @default.
• W2945361239 creator A5069317976 @default.
• W2945361239 creator A5085857115 @default.
• W2945361239 date "2019-06-01" @default.
• W2945361239 modified "2023-10-16" @default.
• W2945361239 title "Identification and quantification of sites of nitration and oxidation in the key matrix protein laminin and the structural consequences of these modifications" @default.
• W2945361239 cites W1496520030 @default.
• W2945361239 cites W1503392039 @default.
• W2945361239 cites W1507170913 @default.
• W2945361239 cites W1797648448 @default.
• W2945361239 cites W1849488471 @default.
• W2945361239 cites W1926950498 @default.
• W2945361239 cites W1931474544 @default.
• W2945361239 cites W1951218128 @default.
• W2945361239 cites W1966797300 @default.
• W2945361239 cites W1967729440 @default.
• W2945361239 cites W1971802256 @default.
• W2945361239 cites W1973592885 @default.
• W2945361239 cites W1976428700 @default.
• W2945361239 cites W1980466934 @default.
• W2945361239 cites W1984895541 @default.
• W2945361239 cites W1985177004 @default.
• W2945361239 cites W1993743744 @default.
• W2945361239 cites W1995290180 @default.
• W2945361239 cites W1995460668 @default.
• W2945361239 cites W2002970663 @default.
• W2945361239 cites W2006026110 @default.
• W2945361239 cites W2019158910 @default.
• W2945361239 cites W2019859522 @default.
• W2945361239 cites W2021914341 @default.
• W2945361239 cites W2022026424 @default.
• W2945361239 cites W2028673251 @default.
• W2945361239 cites W2029849110 @default.
• W2945361239 cites W2033381823 @default.
• W2945361239 cites W2038514844 @default.
• W2945361239 cites W2046076383 @default.
• W2945361239 cites W2048678491 @default.
• W2945361239 cites W2050439069 @default.
• W2945361239 cites W2052098338 @default.
• W2945361239 cites W2054426809 @default.
• W2945361239 cites W2056513004 @default.
• W2945361239 cites W2057797135 @default.
• W2945361239 cites W2058013605 @default.
• W2945361239 cites W2058561090 @default.
• W2945361239 cites W2061935476 @default.
• W2945361239 cites W2064982295 @default.
• W2945361239 cites W2066855036 @default.
• W2945361239 cites W2087947073 @default.
• W2945361239 cites W2089157516 @default.
• W2945361239 cites W2118848380 @default.
• W2945361239 cites W2121191392 @default.
• W2945361239 cites W2121961422 @default.
• W2945361239 cites W2130706354 @default.
• W2945361239 cites W2131564994 @default.
• W2945361239 cites W2137339420 @default.
• W2945361239 cites W2141091417 @default.
• W2945361239 cites W2142207652 @default.
• W2945361239 cites W2195839660 @default.
• W2945361239 cites W2285300636 @default.
• W2945361239 cites W2316918019 @default.
• W2945361239 cites W2325375737 @default.
• W2945361239 cites W2329935557 @default.
• W2945361239 cites W2409280826 @default.
• W2945361239 cites W2465454845 @default.
• W2945361239 cites W2467269011 @default.
• W2945361239 cites W2476542314 @default.
• W2945361239 cites W2561907496 @default.
• W2945361239 cites W2581624131 @default.
• W2945361239 cites W2724889495 @default.
• W2945361239 cites W2756324351 @default.
• W2945361239 cites W2770669941 @default.
• W2945361239 cites W2787052956 @default.
• W2945361239 cites W2884102121 @default.
• W2945361239 cites W2899232733 @default.
• W2945361239 cites W4234012592 @default.
• W2945361239 cites W4291672652 @default.
• W2945361239 doi "https://doi.org/10.1016/j.redox.2019.101226" @default.
• W2945361239 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6543125" @default.
• W2945361239 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31154162" @default.
• W2945361239 hasPublicationYear "2019" @default.
• W2945361239 type Work @default.
• W2945361239 sameAs 2945361239 @default.
• W2945361239 citedByCount "14" @default.
• W2945361239 countsByYear W29453612392019 @default.
• W2945361239 countsByYear W29453612392020 @default.
• W2945361239 countsByYear W29453612392021 @default.
• W2945361239 countsByYear W29453612392022 @default.
• W2945361239 countsByYear W29453612392023 @default.
• W2945361239 crossrefType "journal-article" @default.
• W2945361239 hasAuthorship W2945361239A5020109552 @default.
• W2945361239 hasAuthorship W2945361239A5034929303 @default.
• W2945361239 hasAuthorship W2945361239A5069317976 @default.
• W2945361239 hasAuthorship W2945361239A5085857115 @default.
• W2945361239 hasBestOaLocation W29453612391 @default.
• W2945361239 hasConcept C12554922 @default.

• next