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- W2945382700 abstract "Mesenchymal stem cells (MSC) rely on their ability to integrate physical and spatial signals at load bearing sites to replace and renew musculoskeletal tissues. Designed to mimic unloading experienced during spaceflight, preclinical unloading and simulated microgravity models show that alteration of gravitational loading limits proliferative activity of stem cells. Emerging evidence indicates that this loss of proliferation may be linked to loss of cellular cytoskeleton and contractility. Low intensity vibration (LIV) is an exercise mimetic that promotes proliferation and differentiation of MSCs by enhancing cell structure. Here, we asked whether application of LIV could restore the reduced proliferative capacity seen in MSCs that are subjected to simulated microgravity. We found that simulated microgravity (sMG) decreased cell proliferation and simultaneously compromised cell structure. These changes included increased nuclear height, disorganized apical F-actin structure, reduced expression, and protein levels of nuclear lamina elements LaminA/C LaminB1 as well as linker of nucleoskeleton and cytoskeleton (LINC) complex elements Sun-2 and Nesprin-2. Application of LIV restored cell proliferation and nuclear proteins LaminA/C and Sun-2. An intact LINC function was required for LIV effect; disabling LINC functionality via co-depletion of Sun-1, and Sun-2 prevented rescue of cell proliferation by LIV. Our findings show that sMG alters nuclear structure and leads to decreased cell proliferation, but does not diminish LINC complex mediated mechanosensitivity, suggesting LIV as a potential candidate to combat sMG-induced proliferation loss." @default.
- W2945382700 created "2019-05-29" @default.
- W2945382700 creator A5003470615 @default.
- W2945382700 creator A5005280017 @default.
- W2945382700 creator A5009593426 @default.
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- W2945382700 creator A5070581832 @default.
- W2945382700 creator A5072550096 @default.
- W2945382700 creator A5086254403 @default.
- W2945382700 date "2019-05-15" @default.
- W2945382700 modified "2023-10-16" @default.
- W2945382700 title "Recovery of stem cell proliferation by low intensity vibration under simulated microgravity requires LINC complex" @default.
- W2945382700 cites W1540861556 @default.
- W2945382700 cites W1784155883 @default.
- W2945382700 cites W1913208087 @default.
- W2945382700 cites W1926137791 @default.
- W2945382700 cites W1936547246 @default.
- W2945382700 cites W1968957012 @default.
- W2945382700 cites W1969724199 @default.
- W2945382700 cites W1974489196 @default.
- W2945382700 cites W1976943496 @default.
- W2945382700 cites W1977489392 @default.
- W2945382700 cites W1980596166 @default.
- W2945382700 cites W1982195212 @default.
- W2945382700 cites W1986464954 @default.
- W2945382700 cites W1997083272 @default.
- W2945382700 cites W1998462174 @default.
- W2945382700 cites W2003035047 @default.
- W2945382700 cites W2007211356 @default.
- W2945382700 cites W2010684003 @default.
- W2945382700 cites W2011874786 @default.
- W2945382700 cites W2013760749 @default.
- W2945382700 cites W2014844825 @default.
- W2945382700 cites W2015094353 @default.
- W2945382700 cites W2019582558 @default.
- W2945382700 cites W2021903494 @default.
- W2945382700 cites W2026993081 @default.
- W2945382700 cites W2027781100 @default.
- W2945382700 cites W2032889348 @default.
- W2945382700 cites W2033810023 @default.
- W2945382700 cites W2036145275 @default.
- W2945382700 cites W2037866214 @default.
- W2945382700 cites W2040903601 @default.
- W2945382700 cites W2043224349 @default.
- W2945382700 cites W2043420141 @default.
- W2945382700 cites W2044135793 @default.
- W2945382700 cites W2045041070 @default.
- W2945382700 cites W2045059538 @default.
- W2945382700 cites W2051114659 @default.
- W2945382700 cites W2052236066 @default.
- W2945382700 cites W2056032800 @default.
- W2945382700 cites W2058961479 @default.
- W2945382700 cites W2062197183 @default.
- W2945382700 cites W2062950240 @default.
- W2945382700 cites W2063290334 @default.
- W2945382700 cites W2076827064 @default.
- W2945382700 cites W2080199971 @default.
- W2945382700 cites W2081263377 @default.
- W2945382700 cites W2083954156 @default.
- W2945382700 cites W2085663529 @default.
- W2945382700 cites W2087578326 @default.
- W2945382700 cites W2092402285 @default.
- W2945382700 cites W2092768321 @default.
- W2945382700 cites W2097393454 @default.
- W2945382700 cites W2098168783 @default.
- W2945382700 cites W2098416899 @default.
- W2945382700 cites W2099182595 @default.
- W2945382700 cites W2108553389 @default.
- W2945382700 cites W2112322160 @default.
- W2945382700 cites W2119774673 @default.
- W2945382700 cites W2119962765 @default.
- W2945382700 cites W2130143727 @default.
- W2945382700 cites W2140607398 @default.
- W2945382700 cites W2142016853 @default.
- W2945382700 cites W2146337514 @default.
- W2945382700 cites W2149144495 @default.
- W2945382700 cites W2153344338 @default.
- W2945382700 cites W2156869294 @default.
- W2945382700 cites W2157637111 @default.
- W2945382700 cites W2158111455 @default.
- W2945382700 cites W2162752530 @default.
- W2945382700 cites W2164648414 @default.
- W2945382700 cites W2165375503 @default.
- W2945382700 cites W2170527087 @default.
- W2945382700 cites W2171705946 @default.
- W2945382700 cites W2230606086 @default.
- W2945382700 cites W2232971833 @default.
- W2945382700 cites W2294362632 @default.
- W2945382700 cites W2329686022 @default.
- W2945382700 cites W2341285447 @default.
- W2945382700 cites W2343601324 @default.
- W2945382700 cites W2413223275 @default.
- W2945382700 cites W2415646121 @default.
- W2945382700 cites W2497694566 @default.