FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2945394027> ?p ?o ?g. }

• W2945394027 endingPage "e0217183" @default.
• W2945394027 startingPage "e0217183" @default.
• W2945394027 abstract "Articular cartilage (AC) has poor capacities of regeneration and lesions often lead to osteoarthritis. Current AC reconstruction implies autologous chondrocyte implantation which requires tissue sampling and grafting. An alternative approach would be to use scaffolds containing off-the-shelf allogeneic human articular chondrocytes (HACs). To investigate tolerance of allogeneic HACs by the human immune system, we developed a humanized mouse model implanted with allogeneic cartilage constructs generated in vitro. A prerequisite of the study was to identify a scaffold that would not provoke inflammatory reaction in host. Therefore, we first compared the response of hu-mice to two biomaterials used in regenerative medicine, collagen sponge and agarose hydrogel. Four weeks after implantation in hu-mice, acellular collagen sponges, but not acellular agarose hydrogels, showed positive staining for CD3 (T lymphocytes) and CD68 (macrophages), suggesting that collagen scaffold elicits weak inflammatory reaction. These data led us to deepen our evaluation of the biocompatibility of allogeneic tissue-engineered cartilage by using agarose as scaffold. Agarose hydrogels were combined with allogeneic HACs to reconstruct cartilage in vitro. Particular attention was paid to HLA-A2 compatibility between HACs to be grafted and immune human cells of hu-mice: HLA-A2+ or HLA-A2- HACs agarose hydrogels were cultured in the presence of a chondrogenic cocktail and implanted in HLA-A2+ hu-mice. After four weeks implantation and regardless of the HLA-A2 phenotype, chondrocytes were well-differentiated and produced cartilage matrix in agarose. In addition, no sign of T-cell or macrophage infiltration was seen in the cartilaginous constructs and no significant increase in subpopulations of T lymphocytes and monocytes was detected in peripheral blood and spleen. We show for the first time that humanized mouse represents a useful model to investigate human immune responsiveness to tissue-engineered cartilage and our data together indicate that allogeneic cartilage constructs can be suitable for cartilage engineering." @default.
• W2945394027 created "2019-05-29" @default.
• W2945394027 creator A5007824739 @default.
• W2945394027 creator A5009956396 @default.
• W2945394027 creator A5034160407 @default.
• W2945394027 creator A5052218530 @default.
• W2945394027 creator A5057114540 @default.
• W2945394027 creator A5075889674 @default.
• W2945394027 date "2019-05-20" @default.
• W2945394027 modified "2023-10-13" @default.
• W2945394027 title "Evaluation of the biocompatibility and stability of allogeneic tissue-engineered cartilage in humanized mice" @default.
• W2945394027 cites W1500818418 @default.
• W2945394027 cites W1506104614 @default.
• W2945394027 cites W1536420296 @default.
• W2945394027 cites W1659957480 @default.
• W2945394027 cites W1695503435 @default.
• W2945394027 cites W1954624720 @default.
• W2945394027 cites W1967269017 @default.
• W2945394027 cites W1970910239 @default.
• W2945394027 cites W1972476309 @default.
• W2945394027 cites W1977792312 @default.
• W2945394027 cites W1980114823 @default.
• W2945394027 cites W1980986947 @default.
• W2945394027 cites W1984829970 @default.
• W2945394027 cites W1985603933 @default.
• W2945394027 cites W1987775993 @default.
• W2945394027 cites W1988592246 @default.
• W2945394027 cites W2000548361 @default.
• W2945394027 cites W2002345819 @default.
• W2945394027 cites W2013355638 @default.
• W2945394027 cites W2018169305 @default.
• W2945394027 cites W2024284492 @default.
• W2945394027 cites W2036553623 @default.
• W2945394027 cites W2037707760 @default.
• W2945394027 cites W2044851945 @default.
• W2945394027 cites W2045329100 @default.
• W2945394027 cites W2049279581 @default.
• W2945394027 cites W2050677764 @default.
• W2945394027 cites W2061123096 @default.
• W2945394027 cites W2061356779 @default.
• W2945394027 cites W2065610488 @default.
• W2945394027 cites W2075241858 @default.
• W2945394027 cites W2090489211 @default.
• W2945394027 cites W2091428159 @default.
• W2945394027 cites W2094051996 @default.
• W2945394027 cites W2103546557 @default.
• W2945394027 cites W2128244943 @default.
• W2945394027 cites W2130420151 @default.
• W2945394027 cites W2143953113 @default.
• W2945394027 cites W2145739182 @default.
• W2945394027 cites W2158775673 @default.
• W2945394027 cites W2159465679 @default.
• W2945394027 cites W2167439730 @default.
• W2945394027 cites W2174154237 @default.
• W2945394027 cites W2184277544 @default.
• W2945394027 cites W2187202441 @default.
• W2945394027 cites W2315167384 @default.
• W2945394027 cites W2331733892 @default.
• W2945394027 cites W2334040429 @default.
• W2945394027 cites W250809302 @default.
• W2945394027 cites W2517666626 @default.
• W2945394027 cites W2592704774 @default.
• W2945394027 cites W2598734993 @default.
• W2945394027 cites W2630986599 @default.
• W2945394027 cites W2770197492 @default.
• W2945394027 cites W2903295635 @default.
• W2945394027 cites W2909878391 @default.
• W2945394027 cites W4318599416 @default.
• W2945394027 cites W4362236439 @default.
• W2945394027 doi "https://doi.org/10.1371/journal.pone.0217183" @default.
• W2945394027 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6527235" @default.
• W2945394027 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31107916" @default.
• W2945394027 hasPublicationYear "2019" @default.
• W2945394027 type Work @default.
• W2945394027 sameAs 2945394027 @default.
• W2945394027 citedByCount "14" @default.
• W2945394027 countsByYear W29453940272019 @default.
• W2945394027 countsByYear W29453940272020 @default.
• W2945394027 countsByYear W29453940272021 @default.
• W2945394027 countsByYear W29453940272022 @default.
• W2945394027 countsByYear W29453940272023 @default.
• W2945394027 crossrefType "journal-article" @default.
• W2945394027 hasAuthorship W2945394027A5007824739 @default.
• W2945394027 hasAuthorship W2945394027A5009956396 @default.
• W2945394027 hasAuthorship W2945394027A5034160407 @default.
• W2945394027 hasAuthorship W2945394027A5052218530 @default.
• W2945394027 hasAuthorship W2945394027A5057114540 @default.
• W2945394027 hasAuthorship W2945394027A5075889674 @default.
• W2945394027 hasBestOaLocation W29453940271 @default.
• W2945394027 hasConcept C105702510 @default.
• W2945394027 hasConcept C108586683 @default.
• W2945394027 hasConcept C136229726 @default.
• W2945394027 hasConcept C142724271 @default.
• W2945394027 hasConcept C153911025 @default.
• W2945394027 hasConcept C178790620 @default.
• W2945394027 hasConcept C185592680 @default.
• W2945394027 hasConcept C203014093 @default.
• W2945394027 hasConcept C2777230088 @default.

• next