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• W2945404749 abstract "We investigated the effects of activated charcoal (AC), culture medium strength and various levels of thidiazuron (TDZ) on asymbiotic in vitro seed germination and seedlings development of Pecteilis radiata, an endangered terrestrial orchid. Seeds were inoculated in culture bottle containing quarter-, half- and full-strength Murashige and Skoog (1962) medium (MS) with 0, 1, 2, 4 or 8 μM thidiazuron (TDZ), 3% (w/v) sucrose, and 0.8% (w/v) plant agar. The culture medium strength, TDZ levels, and their interactions had a significant (P < .0001) effect on asymbiotic seed germination. Seeds cultured on full-strength MS medium containing 2 μM TDZ had the highest frequency of asymbiotic seed germination (90.2%) as compared with half-strength MS plus 2 μM TDZ (59.3%) and quarter-strength MS plus 2 μM TDZ (46.8%). The protocorms with rhizoids were subcultured on MS medium containing 0, 1, 2, 3, or 4 g L−1 activated charcoal (AC) for conversion of seedlings. The highest frequency of seedling conversion (91.7%) was obtained on MS medium containing 2 g L−1AC. The in vitro raised seedlings were acclimatized in the greenhouse with an 82% survival rate. The methanolic protocorm extract contained higher amounts of phenolics and flavonoids than shoot and tuber extracts. The methanolic protocorm extract of P. radiata also exhibited strong antioxidant activity." @default.
• W2945404749 created "2019-05-29" @default.
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• W2945404749 date "2019-08-01" @default.
• W2945404749 modified "2023-09-26" @default.
• W2945404749 title "Impact of activated charcoal, culture medium strength and thidiazuron on non-symbiotic in vitro seed germination of Pecteilis radiata (Thunb.) Raf." @default.
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• W2945404749 doi "https://doi.org/10.1016/j.sajb.2019.04.015" @default.
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