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- W2945405110 abstract "We intended to find out in which state fibrin exists in plasma at 37°C. in order to generate fibrin, small amounts of thrombin were added to human plasma containing 125I-fibrinogen. Thereafter, thrombin was quenched with hirudin and 131I-fibrinogen added to observe possible 125I-fibrin-131I-fibrinogen complex formation. Gel filtration of this mixture on sepharose columns equilibrated with plasma revealed 2 peaks. At 20°C, but not at 37°C, peak A eluted in front of the fibrinogen peak contained fibrin-fibrinogen complexes. If isolated fractions of peak A eluted at 20°C were rechromatographed at 37°C, the material was mainly eluted in peak B (= fibrinogen peak). If isolated fractions of peak B eluted at 37°C were rechromatographed at 20°C, part of the 125I-radioactivity representing fibrin was eluted in peak A. as purified des-AB fibrin is eluted in polymeric form at 37°C and as purified des-A fibrin is eluted only in monomeric form at 37°C, we conclude from our experiments that fibrin generating in plasma at 37°c exists in monomeric form. Furthermore, the experiments demonstrate that des-A fibrin does not form complexes with fibrinogen or other proteins at 37°C. (Supported by the DFG)." @default.
- W2945405110 created "2019-05-29" @default.
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- W2945405110 date "1979-07-01" @default.
- W2945405110 modified "2023-10-13" @default.
- W2945405110 title "Soluble Fibrin in Plasma At 37°C Exists as Des-A Fibrin in Monomeric Form" @default.
- W2945405110 doi "https://doi.org/10.1055/s-0038-1665827" @default.
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