Matches in SemOpenAlex for { <https://semopenalex.org/work/W2945546586> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2945546586 abstract "Preeclampsia is one of the complications of pregnancy. Often presenting as new‑onset hypertension and proteinuria after 20 weeks of gestation in a previously normotensive patient, it can progress rapidly to serious complications, and is a leading cause of maternal and perinatal mortality. The present study involved the construction of a fusion protein consisting of a single‑chain antibody variable fragment (scFv) and the retinol‑binding protein 4 (RBP4). and investigated the function of this protein. The MaxCodon™ Optimization Program (v13) was used to optimize the amino acid sequence of the scFv‑RBP4 fusion protein and full‑length splice primers were designed by Detai Bio Tech. The scFv‑RBP4 gene was inserted into a proEM expression vector using double digestion, and the accuracy of the final expression vector was confirmed by restriction enzyme digestion and sequencing. The plasmid was transfected into DH5α competent cells and the plasmid was extracted from cells using a transfection reagent. The plasmid and scFv‑RBP4 fusion protein were purified by nickel‑iminodiacetic acid affinity chromatography. Cell proliferation was determined using the Cell Counting Kit‑8 assay and cell invasion was measured using a Transwell invasion assay. The results from the digestion and sequencing showed that the scFv‑RBP4 fusion protein was constructed correctly and that the purity of the target protein was >90%. The scFv‑RBP4 fusion protein was stably expressed in 293T cells. The scFv‑RBP4 fusion protein was extracted from the 293T cells and functional studies were carried out. The scFv‑RBP4 fusion protein significantly increased the invasion, but not the proliferation, of HTR8/SVneo cells." @default.
- W2945546586 created "2019-05-29" @default.
- W2945546586 creator A5002087255 @default.
- W2945546586 creator A5041324566 @default.
- W2945546586 creator A5045309022 @default.
- W2945546586 creator A5049814813 @default.
- W2945546586 creator A5050760975 @default.
- W2945546586 date "2019-05-22" @default.
- W2945546586 modified "2023-09-30" @default.
- W2945546586 title "Construction and function of a fusion protein consisting of a single‑chain variable fragment and RBP4" @default.
- W2945546586 cites W1501843431 @default.
- W2945546586 cites W150822231 @default.
- W2945546586 cites W1971619188 @default.
- W2945546586 cites W197461666 @default.
- W2945546586 cites W1984970610 @default.
- W2945546586 cites W1989284989 @default.
- W2945546586 cites W2004211176 @default.
- W2945546586 cites W2036015212 @default.
- W2945546586 cites W2041934298 @default.
- W2945546586 cites W2050269811 @default.
- W2945546586 cites W2080671719 @default.
- W2945546586 cites W2092136583 @default.
- W2945546586 cites W2099530492 @default.
- W2945546586 cites W2133792826 @default.
- W2945546586 cites W2138852027 @default.
- W2945546586 cites W2142547020 @default.
- W2945546586 cites W2171162078 @default.
- W2945546586 cites W2416356958 @default.
- W2945546586 cites W2935104625 @default.
- W2945546586 cites W4211054086 @default.
- W2945546586 doi "https://doi.org/10.3892/mmr.2019.10270" @default.
- W2945546586 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6579984" @default.
- W2945546586 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31115584" @default.
- W2945546586 hasPublicationYear "2019" @default.
- W2945546586 type Work @default.
- W2945546586 sameAs 2945546586 @default.
- W2945546586 citedByCount "0" @default.
- W2945546586 crossrefType "journal-article" @default.
- W2945546586 hasAuthorship W2945546586A5002087255 @default.
- W2945546586 hasAuthorship W2945546586A5041324566 @default.
- W2945546586 hasAuthorship W2945546586A5045309022 @default.
- W2945546586 hasAuthorship W2945546586A5049814813 @default.
- W2945546586 hasAuthorship W2945546586A5050760975 @default.
- W2945546586 hasBestOaLocation W29455465861 @default.
- W2945546586 hasConcept C104317684 @default.
- W2945546586 hasConcept C123894998 @default.
- W2945546586 hasConcept C153911025 @default.
- W2945546586 hasConcept C185592680 @default.
- W2945546586 hasConcept C22744801 @default.
- W2945546586 hasConcept C33161422 @default.
- W2945546586 hasConcept C40767141 @default.
- W2945546586 hasConcept C54009773 @default.
- W2945546586 hasConcept C55493867 @default.
- W2945546586 hasConcept C86803240 @default.
- W2945546586 hasConceptScore W2945546586C104317684 @default.
- W2945546586 hasConceptScore W2945546586C123894998 @default.
- W2945546586 hasConceptScore W2945546586C153911025 @default.
- W2945546586 hasConceptScore W2945546586C185592680 @default.
- W2945546586 hasConceptScore W2945546586C22744801 @default.
- W2945546586 hasConceptScore W2945546586C33161422 @default.
- W2945546586 hasConceptScore W2945546586C40767141 @default.
- W2945546586 hasConceptScore W2945546586C54009773 @default.
- W2945546586 hasConceptScore W2945546586C55493867 @default.
- W2945546586 hasConceptScore W2945546586C86803240 @default.
- W2945546586 hasLocation W29455465861 @default.
- W2945546586 hasLocation W29455465862 @default.
- W2945546586 hasLocation W29455465863 @default.
- W2945546586 hasLocation W29455465864 @default.
- W2945546586 hasOpenAccess W2945546586 @default.
- W2945546586 hasPrimaryLocation W29455465861 @default.
- W2945546586 hasRelatedWork W2359175313 @default.
- W2945546586 hasRelatedWork W2368163108 @default.
- W2945546586 hasRelatedWork W2372309604 @default.
- W2945546586 hasRelatedWork W2375453033 @default.
- W2945546586 hasRelatedWork W2384710171 @default.
- W2945546586 hasRelatedWork W2387801567 @default.
- W2945546586 hasRelatedWork W2392734797 @default.
- W2945546586 hasRelatedWork W2394346339 @default.
- W2945546586 hasRelatedWork W2394425474 @default.
- W2945546586 hasRelatedWork W3031052118 @default.
- W2945546586 isParatext "false" @default.
- W2945546586 isRetracted "false" @default.
- W2945546586 magId "2945546586" @default.
- W2945546586 workType "article" @default.