FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2945549915> ?p ?o ?g. }

• W2945549915 endingPage "1504" @default.
• W2945549915 startingPage "1492" @default.
• W2945549915 abstract "Abstract Background Intervertebral disc degeneration is a major cause of chronic low back pain, and excessive loading contributes to intervertebral disc degeneration. However, the lack of an effective bipedal in vivo animal model limits research about this condition. Questions/purposes To evaluate the utility of a new type of bipedal standing mouse model for intervertebral disc degeneration, we asked: (1) Are there spinal degeneration changes in bipedal mice as determined by lumbar disc height, histologic features, and immunohistochemistry measures compared with control mice? (2) Are the bipedal mice comparable to aged mice for simulating the spinal degeneration caused by increased stress? Methods Thirty-two 8-week-old male C57BL/6 mice were divided into experimental and control groups. Based on their hydrophobia, mice in the experimental group were placed in a limited water-containing space (5 mm deep) and were thereby induced to actively take a bipedal standing posture. This was conducted twice a day for a total of 6 hours a day, 7 days a week. Control mice were similarly placed in a limited but water-free space. Video surveillance was used to calculate the percentage of time spent in the bipedal stance for the two groups of mice. Compared with the control group, the percentage of time standing on both feet in the experimental group was higher (48% ± 5%, 95% confidence interval [CI], 42%–54% versus 95% ± 1%, 95% CI, 92%–97%; p < 0.001). Eight mice from both groups were then randomly euthanized at either 6 or 10 weeks and lumbar spine specimens (L3-L6) were collected. The lumbar disc height index (DHI%) of the two groups was compared using micro-CT measurements, and the extent of disc degeneration was assessed based on histologic staining (cartilage endplate height, disc degeneration score) and by immunohistochemistry (Col2a1,CollagenX, matrix metalloprotease-13 [MMP-13], osteocalcin [OCN]). In addition, the histopathologic features of spinal degeneration were compared with 12- and 18-month-old mice. A p value < 0.05 indicated a significant difference. Results Lumbar disc degeneration was aggravated after 10 weeks with the DHI% decreasing (5.0% ± 0.4%; 95% CI, 4.6%–5.5% versus 4.6 ± 0.3%; 95% CI, 4.3%–4.9%; p = 0.011). Histologically, the cartilage endplate height of the experimental group was decreased compared with the control group (30 ± 6 μm; 95% CI, 24–37 μm versus 70 ± 7 μm; 95% CI, 63–79 μm; p < 0.001), and the disc degeneration score was increased (5 ± 1; 95% CI, 4–6 versus 1 ± 1; 95% CI, 0–2; p < 0.001). Expression of Col2a1, vimentin, and aggrecan in the experimental group was decreased compared with the control group, whereas the expressions of collagen X (60% ± 2%; 95% CI, 55%–66% versus 19% ± 3%; 95% CI, 17%–24%; p < 0.001), MMP-13 (54% ± 8%; 95% CI, 49%–61% versus 1% ± 1%; 95% CI, 1%–2%; p < 0.001), and OCN (41% ± 3%; 95% CI, 34%–49% versus 5% ± 1%; 95% CI, 2%–7%, p < 0.001) were increased. The spine degeneration caused by this model was primarily manifested in the degeneration of the annulus fibrosus and facet joints compared with aged mice, whereas the degree of degeneration in the nucleus pulposus tissue and cartilage endplates was mild. Conclusions We believe we have established a noninvasive and effective in vivo bipedal mouse model for studying disc degeneration and biologic signal transduction comparable to that seen in intervertebral disc degeneration. Clinical Relevance This in vivo mouse model of intervertebral disc degeneration can simulate the pathogenesis of spinal degeneration caused by increased stress and this can be used to study questions such as disc herniation in young adults." @default.
• W2945549915 created "2019-05-29" @default.
• W2945549915 creator A5006294670 @default.
• W2945549915 creator A5011459551 @default.
• W2945549915 creator A5024213189 @default.
• W2945549915 creator A5028580598 @default.
• W2945549915 creator A5060678843 @default.
• W2945549915 creator A5073501391 @default.
• W2945549915 creator A5077199030 @default.
• W2945549915 creator A5078898813 @default.
• W2945549915 creator A5086664647 @default.
• W2945549915 date "2019-05-15" @default.
• W2945549915 modified "2023-10-14" @default.
• W2945549915 title "Development and Characterization of a Novel Bipedal Standing Mouse Model of Intervertebral Disc and Facet Joint Degeneration" @default.
• W2945549915 cites W1569274576 @default.
• W2945549915 cites W1966229108 @default.
• W2945549915 cites W1967163791 @default.
• W2945549915 cites W1972411277 @default.
• W2945549915 cites W1972522956 @default.
• W2945549915 cites W1977330311 @default.
• W2945549915 cites W1980151235 @default.
• W2945549915 cites W1982678468 @default.
• W2945549915 cites W1991002331 @default.
• W2945549915 cites W1997828625 @default.
• W2945549915 cites W2003146566 @default.
• W2945549915 cites W2011724488 @default.
• W2945549915 cites W2014256100 @default.
• W2945549915 cites W2015178342 @default.
• W2945549915 cites W2025528234 @default.
• W2945549915 cites W2030506483 @default.
• W2945549915 cites W2037806019 @default.
• W2945549915 cites W2039451700 @default.
• W2945549915 cites W2045200773 @default.
• W2945549915 cites W2052230687 @default.
• W2945549915 cites W2053964636 @default.
• W2945549915 cites W2055272995 @default.
• W2945549915 cites W2075593123 @default.
• W2945549915 cites W2079742829 @default.
• W2945549915 cites W2080394800 @default.
• W2945549915 cites W2088329049 @default.
• W2945549915 cites W2091025894 @default.
• W2945549915 cites W2092013323 @default.
• W2945549915 cites W2097950056 @default.
• W2945549915 cites W2138383018 @default.
• W2945549915 cites W2140476594 @default.
• W2945549915 cites W2167101420 @default.
• W2945549915 cites W2335491049 @default.
• W2945549915 cites W2395513207 @default.
• W2945549915 cites W2405173305 @default.
• W2945549915 cites W2405964370 @default.
• W2945549915 cites W2725477209 @default.
• W2945549915 cites W2883557754 @default.
• W2945549915 doi "https://doi.org/10.1097/corr.0000000000000712" @default.
• W2945549915 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6554109" @default.
• W2945549915 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31135551" @default.
• W2945549915 hasPublicationYear "2019" @default.
• W2945549915 type Work @default.
• W2945549915 sameAs 2945549915 @default.
• W2945549915 citedByCount "31" @default.
• W2945549915 countsByYear W29455499152019 @default.
• W2945549915 countsByYear W29455499152020 @default.
• W2945549915 countsByYear W29455499152021 @default.
• W2945549915 countsByYear W29455499152022 @default.
• W2945549915 countsByYear W29455499152023 @default.
• W2945549915 crossrefType "journal-article" @default.
• W2945549915 hasAuthorship W2945549915A5006294670 @default.
• W2945549915 hasAuthorship W2945549915A5011459551 @default.
• W2945549915 hasAuthorship W2945549915A5024213189 @default.
• W2945549915 hasAuthorship W2945549915A5028580598 @default.
• W2945549915 hasAuthorship W2945549915A5060678843 @default.
• W2945549915 hasAuthorship W2945549915A5073501391 @default.
• W2945549915 hasAuthorship W2945549915A5077199030 @default.
• W2945549915 hasAuthorship W2945549915A5078898813 @default.
• W2945549915 hasAuthorship W2945549915A5086664647 @default.
• W2945549915 hasBestOaLocation W29455499151 @default.
• W2945549915 hasConcept C105702510 @default.
• W2945549915 hasConcept C142724271 @default.
• W2945549915 hasConcept C204787440 @default.
• W2945549915 hasConcept C2775871850 @default.
• W2945549915 hasConcept C2776501849 @default.
• W2945549915 hasConcept C2777755357 @default.
• W2945549915 hasConcept C2779354088 @default.
• W2945549915 hasConcept C2780907711 @default.
• W2945549915 hasConcept C44575665 @default.
• W2945549915 hasConcept C71924100 @default.
• W2945549915 hasConceptScore W2945549915C105702510 @default.
• W2945549915 hasConceptScore W2945549915C142724271 @default.
• W2945549915 hasConceptScore W2945549915C204787440 @default.
• W2945549915 hasConceptScore W2945549915C2775871850 @default.
• W2945549915 hasConceptScore W2945549915C2776501849 @default.
• W2945549915 hasConceptScore W2945549915C2777755357 @default.
• W2945549915 hasConceptScore W2945549915C2779354088 @default.
• W2945549915 hasConceptScore W2945549915C2780907711 @default.
• W2945549915 hasConceptScore W2945549915C44575665 @default.
• W2945549915 hasConceptScore W2945549915C71924100 @default.
• W2945549915 hasIssue "6" @default.
• W2945549915 hasLocation W29455499151 @default.
• W2945549915 hasLocation W29455499152 @default.

• next