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• W2945574747 abstract "Secretory capacity of bovine mammary glands is enabled by a high number of secretory cells and their ability to use a range of metabolites to produce milk components. We isolated RNA from milk fat to measure expression of genes involved in energy-yielding pathways and the unfolded protein response in mammary glands of lactating cows given supplemental energy from protein (PT) and fat (FT) tested in a 2 × 2 factorial arrangement. We hypothesized that PT and FT would affect expression of genes in the branched-chain AA catabolic pathway and tricarboxylic acid (TCA) cycle based on the different energy types (aminogenic versus lipogenic) used to synthesize milk components. We also hypothesized that the response of genes related to endoplasmic reticulum (ER) homeostasis via the unfolded protein response would reflect the increase in milk production stimulated by PT and FT. Fifty-six multiparous Holstein-Friesian dairy cows were fed a basal total mixed ration (34% grass silage, 33% corn silage, 5% grass hay, and 28% concentrate on a dry matter basis) for a 28-d control period. Experimental rations were then fed for 28 d, consisting of (1) low protein, low fat (LP/LF); (2) high protein, low fat (HP/LF); (3) low protein, high fat (LP/HF); or (4) high protein and high fat (HP/HF). To obtain the high-protein (HP) and high-fat (HF) diets, intake of the basal ration was restricted and supplemented isoenergetically (net energy basis) with 2.0 kg/d rumen-protected protein (soybean + rapeseed, 50:50 mixture on dry matter basis) and 0.68 kg/d hydrogenated palm fatty acids on a dry matter basis. RNA from milk fat samples collected on d 27 of each period underwent real-time quantitative PCR. Energy from protein increased expression of BCAT1 (branched-chain amino acid transferase 1) mRNA, but only at the LF level, and tended to decrease expression of mRNA encoding the main subunit of the branched-chain keto-acid dehydrogenase complex. mRNA expression of malic enzyme, a proposed channeling route for AA though the TCA cycle, was decreased by PT, but only at the LF level. Expression of genes associated with de novo fatty acid synthesis was not affected by PT or FT. Energy from fat had no independent effect on genes related to ER homeostasis. At the LF level, PT activated XBP1 (X-box binding protein 1) mRNA. At the HF level, PT increased mRNA expression of the gene encoding GADD34 (growth arrest and DNA damage-inducible 34). These findings support our hypothesis that mammary cells use aminogenic and lipogenic precursors differently for milk component production when dietary intervention alters AA and fatty acid supply. They also suggest that mammary cells respond to increased AA supply through mechanisms of ER homeostasis, dependent on the presence of FT." @default.
• W2945574747 created "2019-05-29" @default.
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• W2945574747 date "2019-07-01" @default.
• W2945574747 modified "2023-10-01" @default.
• W2945574747 title "Expression of genes related to energy metabolism and the unfolded protein response in dairy cow mammary cells is affected differently during dietary supplementation with energy from protein and fat" @default.
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• W2945574747 cites W1672219033 @default.
• W2945574747 cites W1764737285 @default.
• W2945574747 cites W1946555313 @default.
• W2945574747 cites W1973113504 @default.
• W2945574747 cites W1977603207 @default.
• W2945574747 cites W1978755855 @default.
• W2945574747 cites W1990100164 @default.
• W2945574747 cites W1991295161 @default.
• W2945574747 cites W1993688639 @default.
• W2945574747 cites W2005129842 @default.
• W2945574747 cites W2013813851 @default.
• W2945574747 cites W2016698855 @default.
• W2945574747 cites W2031082264 @default.
• W2945574747 cites W2035205542 @default.
• W2945574747 cites W2042246438 @default.
• W2945574747 cites W2049771744 @default.
• W2945574747 cites W2051652691 @default.
• W2945574747 cites W2053500970 @default.
• W2945574747 cites W2053991080 @default.
• W2945574747 cites W2058674722 @default.
• W2945574747 cites W2059043028 @default.
• W2945574747 cites W2062802867 @default.
• W2945574747 cites W2076880493 @default.
• W2945574747 cites W2077784911 @default.
• W2945574747 cites W2081480994 @default.
• W2945574747 cites W2090209417 @default.
• W2945574747 cites W2096007387 @default.
• W2945574747 cites W2098869037 @default.
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• W2945574747 cites W2106416557 @default.
• W2945574747 cites W2107277218 @default.
• W2945574747 cites W2111854688 @default.
• W2945574747 cites W2118507136 @default.
• W2945574747 cites W2119902047 @default.
• W2945574747 cites W2126334171 @default.
• W2945574747 cites W2130062788 @default.
• W2945574747 cites W2133551258 @default.
• W2945574747 cites W2134294998 @default.
• W2945574747 cites W2137289394 @default.
• W2945574747 cites W2141755485 @default.
• W2945574747 cites W2152133873 @default.
• W2945574747 cites W2155337620 @default.
• W2945574747 cites W2156077243 @default.
• W2945574747 cites W2160226323 @default.
• W2945574747 cites W2160857316 @default.
• W2945574747 cites W2167467432 @default.
• W2945574747 cites W2169290856 @default.
• W2945574747 cites W2169685103 @default.
• W2945574747 cites W2170514302 @default.
• W2945574747 cites W2173900734 @default.
• W2945574747 cites W2175084398 @default.
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• W2945574747 doi "https://doi.org/10.3168/jds.2018-15875" @default.
• W2945574747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31103304" @default.
• W2945574747 hasPublicationYear "2019" @default.
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