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• W2945625029 abstract "Mass spectrometry has played a critical role in the identification and quantitation of lipids present in biological extracts. Various strategies have emerged in order to carry out lipidomic studies. These include both shotgun approaches as well as those engaging liquid chromatographic separation of lipid species prior to mass spectrometric analysis. Nonetheless challenges remain at every level of the lipidomic experiment, including extraction of lipids, identification of specific species, and quantitation of the vast array of lipids present in the sample extract. New strategies have emerged to address some of these issues; however, precise quantitation remains a significant challenge. The use of the ratio of the abundance of the molecular ion species to that of an internal standard enables quite accurate assessment of fold changes within complex lipid species without the need for exact quantitation. Challenges continue to remain in terms of availability of reference standard material as well as relevant internal standards." @default.
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• W2945625029 date "2019-01-01" @default.
• W2945625029 modified "2023-09-27" @default.
• W2945625029 title "Overview of Lipid Mass Spectrometry and Lipidomics" @default.
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• W2945625029 doi "https://doi.org/10.1007/978-1-4939-9236-2_6" @default.
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